Abstract
Several major chemicals in the glandular heads of type VI trichomes ofLycopersicon species were identified and quantified by gas chromatography and mass spectrometry. Two normal odd-chained ketones, 2-undecanone (47 ng) and 2-tridecanone (146 ng), and one unknown sesquiterpene (5 ng), comprised approximately 95% of the contents of a gland ofL.hirsutum f.glabratum Mull. In a closely related plant,L.hirsutum Humb. & Bonpl. (LA 361), two unknown insecticidal sesquiterpenes accounted for 6% of the gland contents. Additionally, small amounts of one unknown monoterpene and another unknown sesquiterpene were found in type VI glands of a commercial tomato variety,L.esculentum Mill. Bioassays comparing the gland exudate (by direct contact) and isooctane extracts of glands to neonate larvae ofKeiferia lycopersicella (Walsingham) (Lepidoptera: Gelechiidae) andSpodoptera exigua (Hübner) (Lepidoptera: Noctuidae) indicated that: (1) 2-tridecanone and 2-undecanone were the major insecticidal compounds inL.hirsutum f.glabratum, (2) the two unknown sesquiterpenes inL.hirsutum were acutely toxic to both species, and (3) gland contents in the commercial tomato variety provided only a physical barrier toK.lycopersicella, and were not detrimental toS.exigua. In topical bioassay trials, synthetic mixtures of 2-tridecanone and 2-undecanone (3 ∶1) demonstrated potentiation. Concentrations of these chemicals decreased as trichomes aged. Quantities of insecticidal chemicals and density of type VI trichomes varied with plant age and location within plants.
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References
Abbott, W.S. 1925. A method of computing the effectiveness of an insecticide.J. Econ. Entomol. 18:265–267.
Aina, O.J., Rodriguez, J.G., andKnavel, D.E. 1972. Characterizing resistance toTetranychus urticae in tomato.J. Econ. Entomol. 65:641–645.
Beckman, C.H., Mueller, W.C., andMcHardy, W.E. 1972. The localization of stored phenols in plant hairs.Physiol. Plant Pathol. 2:69–74.
Buckingham, J. (ed.). 1982. Dictionary of Organic Compounds, 5th ed. Chapman and Hall, New York.
Croteau, R.,andLoomis, W.D. 1972. Biosynthesis of mono- and sesquiterpenes in peppermint from mevalonate-2-14C.Phytochemistry 11:1055–1066.
Dean, J.A. (ed.). 1979. Lange's Handbook of Chemistry, 12th ed. McGraw-Hill, New York.
Dimock, M.B., andKennedy, G.G. 1983. The role of glandular trichomes in the resistance ofLycopersicon hirsutum f.glabratum toHeliothis zea.Entomol. Exp. Appl. 33:263–268.
Dimock, M.B., Kennedy, G.G., andWilliams, W.G. 1982. Toxicity studies of analogs of 2-tridecanone, a naturally occurring toxicant from a wild tomato.J. Chem. Ecol. 8:837–842.
Duffey, S.S, andIsman, M.B. 1981. Inhibition of insect larval growth by phenolics in glandular trichomes of tomato leaves.Experientia 37:574–576.
Fery, R.L., andKennedy, G.G. 1983. Inheritance of a factor inLycopersicon hirsutum conditioning resistance to the tobacco hornworm (Manduca sexto).Hortic. Sci. 18:169 (abstract).
Fery, R.L., Kennedy, G.G., andSorenson, C.F. 1984. Genetic analyses of 2-tridecanone concentration and resistance to the tobacco hornworm (Manduca sexto) and the Colorado potato beetle (Leptinotarsa decemlineata) inLycopersicon species.Hortic. Sci. 19:562 (abstract).
Finney, D.J. 1971. Quantal responses to mixtures, pp. 230–282,in D.J. Finney (ed.)Probit Analysis. Cambridge University Press, Cambridge.
Helwig, J.T., andCouncil, K.A. (eds.). 1979. SAS User's Guide. Statistical Analysis Systems Institute, Inc., Cary, North Carolina.
Kelsey, R.G., Reynolds, G.W., andRodriguez, E. 1984. The chemistry of biologically active constituents secreted and stored in plant glandular trichomes. pp. 187–244,in E. Rodriguez, P.L. Healey, and I. Meta (eds.). Biology and Chemistry of Plant Trichomes. Plenum Press, New York.
Kennedy, G.G., andDimock, M.B. 1983. 2-Tridecanone: A natural toxicant in a wild tomato responsible for insect resistance, pp. 123–128,in J. Miyamato and P.C. Kearney (eds.). Pesticide Chemistry: Human Welfare and the Environment, Vol. 2. Pergamon Press, Tokyo.
Kennedy, G.G., andHenderson, W.R. 1978. A laboratory assay for resistance to the tobacco hornworm inLycopersicon andSolanum spp.J. Am. Soc. Hortic. Sci. 103:334–336.
Kennedy, G.G., andSorenson, C.F. 1985. Role of glandular trichomes in the resistance ofLycopersicon hirsutum f.glabratum to Colorado potato beetle (Coleoptera: Chrysomelidae).J. Econ. Entomol. 78:547–551.
Langenheim, J.H., Osmond, C.B., Brooks, A., andFerrar, P.J. 1984. Photosynthetic responses to light in seedlings of selected Amazonian and Australian rainforest tree species.Oecologia (Berlin) 63:215–224.
Lin, S.Y.H., andTrumble, J.T. 1985. Influence of temperature and tomato maturity on development and survival ofKeiferia lycopersicella (Lepidoptere: Gelechiidae).Environ. Entomol. 14:855–858.
Lin, S.Y.H., andTrumble, J.T. 1986. Resistance in wild tomatoes to larvae of a specialist herbivore,Keiferia lycopersicella. Entomol. Exp. Appl. 41:53–60.
Luckwill, L.C. 1943. The genusLycopersicon: An historical, biological, and toxonomic survey of the wild and cultivated tomatoes. Aberdeen University Studies No. 120, Aberdeen University Press, Aberdeen, Scotland.
Matkin, O.A., andChandler, P.A. 1957. The U.C.-type soil mixes, pp. 68–85,in K.F. Baker (ed.). The U.C. System for Producing Healthy Container Grown Plants. California Experiment Station Manual 23, University of California, Division of Agricultural Science. Berkeley.
Patana, R. 1969. Rearing cotton insects in the laboratory. U.S. Department of Agriculture Product Research Report 108:1–6.
Rodriguez, J.R., Knavel, D.E., andAina, O.J. 1972. Studies in the resistance of tomatoes to mites.J. Econ. Entomol. 65:50–53.
Schuster, D.J. 1977. Effects of tomato cultivars on insect damage and chemical control.Fla. Entomol. 60:227–232.
Schwartz, R.F., andSnyder, J.C. 1983. Characterization of resistance to tobacco hornworn inLycopersicon leaflets.Hortic. Sci. 18:170 (abstract).
Silverstein, R.M., Bassler, G.C., andMorrill, T.C. 1974. Spectrometric Identification of Organic Compounds. John Wiley & Sons, New York.
Snyder, J.C., andCarter, C.D. 1984. Leaf trichomes and resistance ofLycopersicon hirsutum andL. esculentum to spider mites (Tetranychus urticae).J. Am. Soc. Hortic. Sci. 109:837–843.
Soost, R.K., Scora, R.W., andSims, J.J. 1968. Contribution to the chromatographic analysis of leaf oils in the genusLycopersicon.J. Am. Soc. Hortic. Sci. 92:568–571.
Turner, J.C., Hemphill, J.K., andMahlberg, P.G. 1980. Trichomes and cannabinoid content of developing leaves and bracts ofCannabis sativa L (Cannabaceae).Am. J. Bot. 67:1397–1406.
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Lin, S.Y.H., Trumble, J.T. & Kumamoto, J. Activity of volatile compounds in glandular trichomes ofLycopersicon species against two insect herbivores. J Chem Ecol 13, 837–850 (1987). https://doi.org/10.1007/BF01020164
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DOI: https://doi.org/10.1007/BF01020164