Zusammenfassung
Koloniebildende Zellen (CFU-C) aus dem Blut und Koloniestimulierte Aktivität aus dem „feeder layer“ peripherer Blutleukozyten (Leukozyten-CSA) wurden in einem halbflüssigen Agarkultur-System bei 69 Normalpersonen untersucht. Gesunde Freiwillige wurden nach Geschlecht und Alter (20–45 Jahre und älter als 60 Jahre) in Gruppen unterteilt. Der Anteil der zirkulierenden CFU-C war bei jungen Frauen (20–45 Jahre) signifikant niedriger als in der Gruppe älterer Männer (älter als 60 Jahre), jedoch waren die Unterschiede zwischen den übrigen Gruppen nicht signifikant. Leukozytäre CSA unterschied sich in den verschiedenen Gruppen nicht signifikant. Bei 5 jungen Männern wurden zirkulierende CFU-C am selben Tag morgens um 8.00 und nachmittags um 16.00 Uhr untersucht: Es ergaben sich keine unterschiedlichen Werte. Bei 18 Personen wurden die Untersuchungen nach längeren Zeitintervallen wiederholt: Die Anzahl der gebildeten Kolonien variierte maximal um das Fünffache. Plasma und segmentkernige Granulozyten, wie sie im angewandten Kultursystem üblich waren, inhibierten das Wachstum der Kolonien nicht. In den meisten Fällen ließen sich in doppelschichtigen Kultursystemen höhere Koloniezahlen erreichen als in einschichtigen, jedoch schienen die „feeder layer“ einiger normaler Personen das Koloniewachstum zu inhibieren.
Summary
Blood colony-forming cells (CFU-C) and colony-stimulating activity obtained from feeder layers of peripheral blood leucocytes (leucocyte CSA) have been studied in 69 normal subjects by means of semisolid agar culture system. Groups of normal volunteers were selected according to sex and age (20 to 45 and older than 60 years) and the results compared. The mean number of circulating CFU-C was significantly lower in young women (20–45 years old) than in males over 60 years of age, but no differences were found among the other age and sex groups. Leucocyte CSA did not significantly differ among these groups. In 5 young males the blood CFU-C did not show significant variations at 8 AM and at 4 PM of the same day. When the study was repeated in 18 subjects at longer time intervals, the number of colonies showed a maximum fivefold variation. The amount of plasma and polymorphonuclear granulocytes present in our culture system did not inhibit the colony growth. In most cases, double layer cultures grow a higher number of colonies than single layer, but feeder layers of some normal subject seem to inhibit the colony growth.
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Supported by a grant from the Italian National Research Council (CNR) CT 76.01323.04/115. 2235-1976/1977
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Ponassi, A., Morra, L., Bonanni, F. et al. Normal range of blood colony-forming cells (CFU-C) in humans: Influence of experimental conditions, age, sex, and diurnal variations. Blut 39, 257–263 (1979). https://doi.org/10.1007/BF01013218
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DOI: https://doi.org/10.1007/BF01013218