Abstract
Olfactory perception of pheromonal enantiomers by male and female Douglas-fir beetles,Dendroctonus pseudotsugae Hopk. (Coleoptera: Scolytidae), was investigated by electrophysiological techniques and behavioral bioassays. Electroantennograms (EAGs) and single-cell responses indicated both sexes to be more responsive to racemic frontalin and the (−)-enantiomer at lower dosages. At higher dosages, little difference was noted in responses to either enantiomer. However, response to the racemic mixture at higher dosages was slightly greater than responses to either enantiomer alone. In laboratory behavioral bioassays, responses to low concentrations of (−)-frontalin and the racemic mixture exceeded response to the (+)-enantiomer alone. At a higher concentration, responses to the racemic mixture or either enantiomer alone did not differ. The results indicate that separate enantiomer-specific acceptors may exist on the same pheromone receptor cell.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Boeckh, J. 1962. Elektrophysiologische Untersuchungen an einzelnen Geruchs-Rezeptoren auf den Antennen des Totegräbers (Necrophorus, Coleoptera).Z. Vergl. Physiol. 46:212–248.
Dickens, J.C. 1979. Electrophysiological investigations of olfaction in bark beetles.Mitt. Schweiz. Entomol. Ges. 52:203–216.
Dickens, J.C., Gutmann, A., Payne, T.L., Ryker, L.C., andRudinsky, J.A. 1983. Antennal olfactory responsiveness of the Douglas-fir beetle,Dendroctonus pseudotsugae Hopkins (Coleoptera: Scolytidae) to pheromones and host odors.J. Chem. Ecol. 9:1383–1395.
Dickens, J.C., andPayne, T.L. 1977. Bark beetle olfaction: Pheromone receptor system inDendroctonus frontalis.J. Insect Physiol. 23:481–489.
Dickens, J.C., Payne, T.L., Ryker, L.C., andRudinsky, J.A. 1984. Single cell responses of the Douglas-fir beetle,Dendroctonus pseudotsugae Hopkins (Coleoptera: Scolytidae), to pheromones and host odors.J. Chem. Ecol. 10:583–600.
Hansen, K. 1983. Reception of bark beetle pheromone in the predaceous clerid beetle,Thanasimus formicarius (Coleoptera: Cleridae).J. Comp. Physiol. 105:371–378.
Jantz, O.K., andJohnsey, R.L. 1964. Determination of sex of the Douglas-fir beetleDendroctonus pseudotsugae Hopkins (Coleoptera: Scolytidae).Can. Entomol. 96:1327–1329.
Jantz, O.K., andRudinsky, J.A. 1965. Laboratory and field methods for assaying olfactory responses of the Douglas-fir beetle,Dendroctonus pseudotsugae Hopkins.Can. Entomol. 97:935–941.
Kafka, W.A., Ohloff, G., Schneider, D., andVareschi, E. 1973. Olfactory discrimination of two enantiomers of 4-methyl-hexanoic acid by the migratory locust and the honeybee.J. Comp. Physiol. 87:277–284.
Kinzer, G.W., Fentman, A.F., Jr., Page, T.F., Jr., Foltz, R.L., Vité, J.P., andPitman, G.B. 1969. Bark beetle attractants: Identification, synthesis and field bioassay of a new compound isolated fromDendroctonus.Nature 221:477–478.
Kinzer, G.W., Fentiman, A.F., Jr., Foltz, R.L., andRudinsky, J.A. 1971. Bark beetle attractants: 3-Methyl-2-cyclohexen-1-one isolated fromDendroctonus pseudotsugae.J. Econ. Entomol. 64:970–971.
Mustaparta, H., Augst, M.E., andLanier, G.N. 1980. Receptor discrimination of enantiomers of the aggregation pheromone ipsdienol, in two species ofIps.J. Chem. Ecol. 8:689–701.
Mori, K. 1975. Synthesis of optically active forms of frontalin, the pheromone ofDendroctonus bark beetles.Tetrahedron 31:1381–1384.
O'Connell, R.J. 1975. Olfactory receptor responses to sex pheromone components in the redbanded leafroller moth.J. Gen. Physiol. 65:179–205.
Ostle, B. 1963. Statistics in Research. Iowa State University Press, Ames, Iowa, xv+585 pp.
Payne, T.L., Richerson, J.V., Dickens, J.C., West, J.R., Mori, K., Berisford, C.W., Hedden, R.L., Vité, J.P., andBlum, M.S. 1982. Southern pine beetle: Olfactory receptor and behavior discrimination of enantiomers of the attractant pheromone frontalin.J. Chem. Ecol. 8:873–881.
Pitman, G.B., andVité, J.P. 1970. Field response ofDendroctonus pseudotsugae (Coleoptera: Scolytidae) to synthetic frontalin.Ann. Entomol. Soc. Am. 63:661–664.
Renwick, J.A.A., andDickens, J.C. 1979. Control of pheromone production in the bark beetle,Ips cembrae.Physiol. Entomol. 4:377–381.
Renwick, J.A.A., Hughes, P.R., andKrull, I.S. 1976. Selective production ofcis- andtrans-verbenol from (−)- and (+)-α-pinene by a bark beetle.Science 191:199–201.
Rudinsky, J.A. 1973. Multiple function of Douglas-fir beetle pheromone 3-methyl-2-cyclohexen-1-one.Environ. Entomol. 2:580–585.
Rudinsky, J.A., andRyker, L.C. 1977. Olfactory and auditory signals mediating behavioral patterns of bark beetles.Colloq. Int. C.N.R.S. 265:196–209.
Rudinsky, J.A., Kinzer, G.W., Fentiman, A.F., Jr., andFoltz, R.L. 1972.Trans-verbenol isolated from Douglas-fir beetle: Laboratory and field bioassays in Oregon.Environ. Entomol. 1:485–488.
Rudinsky, J.A., Morgan, M.E., Libbey, L.M., andPutnam, T.B. 1974. Additional components of the Douglas-fir beetle (Col. Scolytidae) aggregative pheromone and their possible utility in pest control.Z. Angew. Entomol. 76:65–77.
Ryker, L.C., Libbey, L.M., andRudinsky, J.A. 1979. Comparison of volatile compounds and stridulation emitted by the Douglas-fir beetle from Idaho and western Oregon populations.Environ. Entomol. 8:789–798.
Schneider, D. 1957. Elektrophysiologische Untersuchungen von Chemo-und Mechanorezeptoren der Antenne des SeidenspinnersBombyx mori L. Z.Vergl. Physiol. 40:8–41.
Silverstein, R.M. 1979. Enantiomeric composition and bioactivity of chiral semiochemicals in insects, pp. 133–146, in F.J. Ritter (ed.). Chemical Ecology: Odour Communication in Animals. Elsevier/North-Holland Biomedical Press, Amsterdam.
Städler, E. 1974. Host plant stimuli affecting behavior of the eastern spruce budworm.Entomol. Exp. Appl. 17:176–188.
Stewart, T.E., Plummer, E.L., McCandless, L.L., West, J.R., andSilverstein, R.M. 1977. Determination of enantiomer composition of several bicyclic ketal pheromone components.J. Chem Ecol. 3:27–43.
Vité, J.P., Pitman, G.B., Fentiman, A.F., Jr., andKinzer, G.W. 1972. 3-Methyl-2-cyclohexenl-ol isolated fromDendroctonus.Naturwissenschaften 59:469.
Wadhams, L.J., Angst, M.E., andBlight, M.M. 1982. Responses of the olfactory receptors ofScolytus scolytus (F.) (Coleoptera: Scolytidae) to the stereoisomers of 4-methyl-3-heptanol.J. Chem. Ecol. 8:477–492.
Wood, D.L., Browne, L.E., Ewing, B., Lindahl, K., Bedard, W.D., Tilden, P.E., Mori, K., Pitman, G.B., andHughes, P.R. 1976. Western pine beetle: Specificity among enantiomers of male and female components of an attractant pheromone.Science 192:896–898.
Author information
Authors and Affiliations
Additional information
Texas Agricultural Experiment Station paper No. TA-19841. This research was supported in part by Mclntire-Stennis Project 1525, the U.S. Department of Agriculture-sponsored program entitled “The Expanded Southern Pine Beetle Research and Applications Program” through TAES-SEA-CR grant 904-15-4, and NSF grant PCN 79-21708 to Oregon State University. The findings, opinions and recommendations expressed herein are those of the authors and not necessarily those of the U.S. Department of Agriculture. All programs and information of the Texas Agricultural Experiment Station are available without regard to race, ethnic origin, religion, sex, or age.
Mention of a trademark, proprietary product or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that may also be suitable.
Rights and permissions
About this article
Cite this article
Dickens, J.C., Payne, T.L., Ryker, L.C. et al. Multiple acceptors for pheromonal enantiomers on single olfactory cells in the Douglas-fir beetle,Dendroctonus pseudotsugae Hopk. (Coleoptera: Scolytidae). J Chem Ecol 11, 1359–1370 (1985). https://doi.org/10.1007/BF01012137
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01012137