Synopsis
An electronic device is described for the automatic correction of fluorescence emission spectra obtained by digital microspectrofluorometry based on multichannel scaling and single photon detection as described previously. This device consists of: (a) an arithmetic unit for the correction of the spectral values and for curve integration processes; (b) a circuit that operates directly on data in the memory of the multi-channel analyser by subtracting from them a pre-established value corresponding to the back-ground; and (c) an averaging unit for calculating a mean for the spectral value.
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Mazzini, G., Bottiroli, G. & Prenna, G. An electronic device for the automatic correction of fluorescence emission spectra. Histochem J 7, 291–297 (1975). https://doi.org/10.1007/BF01003598
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DOI: https://doi.org/10.1007/BF01003598