Summary
The ‘total protein staining’ of biological specimens with the electrostatically binding Naphthol Yellow S or the covalently binding dinitrofluorobenzene must be interpreted as methods which yield data on the specific amino acid pool of the proteins concerned. Both dyes bind to certain free amino-acid side-chains, giving different dye-protein ratios for various proteins. In the presence of DNA, dinitrofluorobenzene stains all proteins present in cell nuclei, whereas Naphthol Yellow S only stains the majority of the non-histone proteins. When protein staining methods are combined with the Feulgen-Pararosaniline (SO2) procedure for DNA, decreased Feulgen-DNA contents were measured in dinitrofluorobenzene-stained isolated nuclei and lymphocytes.
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Tas, J., James, J. Quantitative cytochemistry of nuclear and cytoplasmic proteins using the Naphthol Yellow S and dinitrofluorobenzene staining methods. Histochem J 13, 711–716 (1981). https://doi.org/10.1007/BF01003283
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DOI: https://doi.org/10.1007/BF01003283