Abstract
Immunological assays for the detection of picogram amounts of gibberellins A1, A3, A4, A7, A9 and A20 are described. Antisera of high affinity (Ka over 1010 l mol-1) were raised in rabbits by immunization against carboxyl-coupled gibberellin-bovine-serum-albumin conjugates and used in combination with tritiated gibberellins or gibberellin derivatives of high specific radioactivity (over 1014 Bq mol-1). The assays allow gibberellin quantitation in as little as 1 mg of vegetative plant tissue.
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Abbreviations
- GA:
-
gibberellin
- GA3 :
-
gibberellic acid
- TLC:
-
thin-layer chromatography
References
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Atzorn, R., Weiler, E.W. The immunoassay of gibberellins. Planta 159, 1–6 (1983). https://doi.org/10.1007/BF00998806
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DOI: https://doi.org/10.1007/BF00998806