Summary
The time course in which radioactivity is found in maternal serum after a single injection of Na2 35SO4 may be drastically changed under the influence of drugs. This must influence the rate of incorporation of35S labelled precursors into the GAG fractions of embryonic tissue. Therefore it is not possible to draw any conclusions from in vivo labelling experiments unless at least the change in the radioactivity of the maternal serum has been taken into consideration.
A method is presented which allows an evaluation of an effect of drugs on GAG also in such cases in which the availability of the isotope from the maternal organism is changed: instead of measuring the rate of incorporation into the total GAG, a subfractionation of GAG according to their anionic behaviour is performed. In a routine assay three fractions of GAG are obtained after fractionation on ECTEOLA-cellulose. The ratio of incorporation of radioactively labelled precursors into the different subfractions is taken as indication of an effect of a drug on GAG metabolism. As an example the effect of vitamin A and Na-salicylate is analysed.
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Bitter, T., Muir, H. M.: A modified uronic acid carbazole reaction. Analyt. Biochem.4, 330 (1962).
Kochhar, D. M., Larsson, K. S., Boström, H.: Embryonic uptake of S35-sulfate: Change in level following treatment with some teratogenic agents. Biol. Neonat. (Basel)12, 41 (1968).
Mathews, M. B.: The macromoleculare organization of connective tissue. In: Chemical Physiology of Mucopolysaccharides. Boston: Little, Brown & Co. 1968.
Nanda, R.: The role of sulfated mucopolysaccharides in cleft palate production. Teratology3, 237 (1970).
Perumal, A. S., Anantha Samy, T. S., Lakshmanan, M. R., Jungalwala, F. B., Rama Rao, P. B.: Biosynthesis of sulfated mucopolysaccharides in relation to vitamins A and K. Biochim. biophys. Acta (Amst.)124, 95 (1966).
Robertson, D. W.: The effect of sodium tolbutamide on the uptake of sodium-35S-sulfate and35S-Methionine in inbred strains of mice. Teratology1, 387 (1968).
Schimmelpfennig, K., Baumann, I., Kaufmann, C.: Studies on glycosaminoglycans (GAG) in mammalian embryonic tissue. I. A micromethod for the fractionation and characterisation of GAG according to their anionic behaviour. Naunyn-Schmiedebergs Arch. Pharmak. (in press 1971).
- - - Studies on glycosaminoglycans in mammalian embryonic tissue. II. Influence of vitamin A and Na-salicylate on embryonic GAG. Naunyn-Schmiedebergs Arch. Pharmak. (in press 1972).
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This work was generously supported by grants from the Deutsche Forschungsgemeinschaft given to the Sonderforschungsbereich 29, “Embryonale Entwicklung und Differenzierung (Embryonal-Pharmakologie)”.
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Schimmelpfennig, K. Problems connected with in vivo labelling of embryonic glycosaminoglycans with Na2 35SO4 in teratological studies. Naunyn-Schmiedebergs Arch. Pharmak. 271, 320–324 (1971). https://doi.org/10.1007/BF00997226
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DOI: https://doi.org/10.1007/BF00997226