Abstract
Two species of termites,Reticulitermes (lucifugus) grassei andR. (l.) banyulensis, show a high degree of aggressivity toward each other. The epicuticular signature, recognized by contact, can be extracted using organic solvents, and the removal of the signature abolished all types of aggressive behavior. The signature can be transferred to lures, where it triggers interspecies aggression. It was found to be mainly present in the apolar fraction of the cuticular extracts, which contained only hydrocarbons, are determined by GC/MS techniques. Chemical recognition contributes towards isolation of the two species belonging to theR. lucifugus complex.
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Bagnères, A.G. 1989. Les Hydrocarbures Cuticulaires des Insectes Sociaux. Détermination et Rôle dans la Reconnaissance Spécifique Coloniale et Individuelle. Thèse de Doctorat de l'Université P. et M. Curie. 250 pp.
Bagnères, A.G., Clément, J.L., Lange, C., andJoulie, C. 1988. Les hydrocarbures cuticulaires desReticulitermes français: Variations spécifiques et coloniales.Actes Coll. Ins. Soc. 4:34–42.
Bagnères, A.G., andMorgan, E.D. 1991. The postpharyngeal glands and the cuticle of Formicidae contain the same characteristic hydrocarbons.Experientia 47:106–111.
Bagnères, A.G., Clément, J.L., Blum, M.S., Severson, R.F., Joulie, C., andLange, C. 1990. Cuticular hydrocarbons and defensive compounds ofReticulitermes flavipes (Kollar) andR. santonensis (Feytaud): Polymorphism and chemotaxonomy.J. Chem. Ecol. 16:3213–3244.
Bagnères, A.G., Errard, C., Mulheim, C., Joulie, C., andLange, C. 1991. Induced mimicry of colony odors in ants.J. Chem. Ecol. 17:1641–1664.
Blomquist, G.J., andJackson, L.L. 1976. Insect waxes, pp. 201–233,in P.E. Kolattukudy (ed.). Chemistry and Biochemistry. Elsevier, Amsterdam.
Blomquist, G.J., Howard, R.W., andMcDaniel, C.A. 1979. Structure of the cuticular hydrocarbons of the termiteZootermopsis angusticollis (Hagen).Insect Biochem. 9:365–370.
Blomquist, G.J., Howard, R.W., McDaniel, C.A., Remaley, S., Dwyer, L.A., andNelson, D.R. 1980. Application of methoxymercuration-demercuration followed by mass spectrometry as a convenient microanalytical technique for double-bond location in insect-derived alkenes.J. Chem. Ecol. 6:257–269.
Blomquist, G.J., Nelson, D.R., andde Renobales, M. 1987. Chemistry, biochemistry, and physiology of insect cuticular lipids.Arch. Ins. Biochem. Physiol. 6:227–265.
Blum, M.S. 1987. Specificity of pheromonal signals: A search for its recognitive bases in terms of a unified chemisociality, pp. 401–405,in J. Peperny (ed.). Chemistry and Biology of Social Insects, Verlag, München.
Bonavita-Cougourdan, A., Clément, J.L., andLange, C. 1987. Nestmate recognition: The role of cuticular hydrocarbons in the antCamponotus vagus.J. Entomol. Sci. 22:1–10.
Bonavita-Cougourdan, A., Clément, J.L., andLange, C. 1989. The role of cuticular hydrocarbons in recognition of larvae by workers of the antCamponotus vagus: Changes in the chemical signature in response to social environment (Hymenoptera: Formicidae).Sociobiology 16:49–74.
Brown, W.V., Watson, J.A.L., Carter, F.L., Lacey, M.J., Barett, R.A., andMcDaniel, C.A. 1990. Preliminary examination of cuticular hydrocarbons of worker termites as chemotaxonomic characters for some australian species ofCoptotermes (Isoptera: Rhinotermitidae).Sociobiology 16:305–328.
Carlson, D.A., andService, M.W. 1980. Identification of mosquitoes ofAnopheles gambiae species complex A and B by analysis of cuticular components.Science 207:1089–1091.
Castner, J.L., andNation, J.L. 1986. Cuticular lipids for species recognition of mole crickets (Orthoptera: Gryllotalpidae): II.Scapteriscus abbreviatus, S. acletus, S. vicinus, S. sp., andNeocurtilla hexadactyla.Arch. Ins. Biochem. Physiol. 3:127–134.
Clément, J.L. 1978. L'agression interspécifique et intraspécifique des espèces françaises du genreReticulitermes (Isoptère).C.R. Acad. Sci. Paris 286:351–354.
Clément, J.L. 1981a. Comportement de reconnaissance individuelle dans le genreReticulitermes (Isoptera).C.R. Acad. Sci. Paris 292:931–933.
Clément, J.L. 1981b. Autoécologie, distance phénotypiques et génétiques entre population des termites du complexeReticulitermes lucifugus.Vie Milieu 31:261–270.
Clément, J.L. 1982. Signaux de contact responsables de l'agression interspécifique des termites du genreReticulitermes (Isoptères).C.R. Acad. Sci. Paris 294:635–638.
Clément, J.L. 1986. Open and closed societies inReticulitermes termites (Isoptera, Rhinotermitidae): Geographic and seasonal variations.Sociobiology 11:311–323.
Clément, J.L., Lange, C., Blum, M.S., Howard, R.W., andLloyd, H. 1985. Chimosystematique du genreReticulitermes (Isoptères) aux U.S.A. et en Europe.Actes Coll. Ins. Soc. 2:123–131.
Clément, J.L., Howard, R.W., Blum, M.S., andLloyd, H. 1986. L'isolement spécifique des termites du genreReticulitermes (Isoptera) du sud-est des Etats-Unis. Mise en évidence grâce à la chimie et au comportement d'une espèce jumelle deR. virginicus=R. malletei sp. nov. et d'une semi-espèce deR. flavipes.C. R. Acad. Sci. Paris 302:67–70.
Clément, J.L., Lemaire, M., Nagnan, P., Escoubas, P., Baonères, A.G., andJoulie, C. 1988. Chemical ecology of European termites of the genusReticulitermes: Allomones, pheromones and kairomones.Sociobiology 14:165–174.
Corbara, B., andErrard, C. 1991. The organisation of artificial heterospecific ant colonies. The case ofManica rubida/Formica selysi (Myrmicinae, Formicinae) association: Mixed colony or parallel colonies.Behav. Proc. 23:75–87.
Espelie, K.E., andHermann, H.R. 1988. Congruent cuticular hydrocarbons: Biochemical convergence of a social wasp, an ant and a host plant.Biochem. Syst. Ecol. 16:505–508.
Espelie, K.E., Wenzel, J.W., andChang, G. 1990. Surface lipids of social waspPolistes metricus say and its nest and nest pedicel and their relation to nestmate recognition.J. Chem. Ecol. 16:2229–2241.
Fielde, A.M. 1903. Artificial mixed nests of ants.Biol. Bull. V(6):320–325.
Fielde, A.M. 1904. Power of recognition among ants.Biol. Bull. VII(5):227–250.
Fielde, A.M. 1905. The progressive odor of ants.Biol. Bull. X(1):1–15.
Franks, N., Blum, M.S., Smith, R.K., andAllies, A.B. 1990. Behavior and chemical disguise of cuckoo antLeptothorax kutteri in relation to its host.J. Chem. Ecol. 16:1431–1444.
Goldberg, J. 1971. Mise en évidence du transport d'eau par le termite de Saintonge.C.R. Acad. Sci. Paris 272:2820–2822.
Grunshawn, J.P., Guermouche, H., Guermouche, S., Jago, N.D., Jullien, R., Knowles, E., andPerez, F. 1990. Chemical taxonomic studies of cuticular hydrocarbons in locusts of theSchistocerca americana complex: Chemical relationships between New World and Old World species.J. Chem. Ecol. 16:2835–2858.
Guille-Escuret, G. 1981. Contribution à la sociologie du genreReticulitermes (Isoptera: Rhino-termitidae) en charente-maritime. Thèse de Doctorat 3ème cycle, U.P.M.C.
Haverty, M.I., andThorne, B.L. 1989. Agonistic behavior correlated with hydrocarbon pheno-types in dampwood termites,Zootermopsis (Isoptera: Termopsidae).J. Ins. Behav. 2:523–543.
Haverty, M.I., Page, M., Nelson, L.J., andBlomquist, G.J. 1988. Cuticular hydrocarbons of dampwood termites,Zootermopsis: Intra- and intercolony variation and potential as taxonomic characters.J. Chem. Ecol. 14:1035–1058.
Haverty, M.I., Nelson, L.J., andPage, M. 1990a. Cuticular hydrocarbons of four populations ofCoptotermes formosanus Shiraki in the United States: Similarities and origins of introductions.J. Chem. Ecol. 16:1635–1647.
Haverty, M.I., Thorne, B.L., andPage, M. 1990b. Surface hydrocarbon components of two species ofNasutitermes from Trinidad.J. Chem. Ecol. 16:2441–2450.
Haverty, M.I., Nelson, L.J., andPage, M. 1991. Preliminary investigations of the cuticular hydrocarbons from North AmericanReticulitermes and tropical and subtropicalCoptotermes (Isoptera: Rhinotermitidae) for chemostaxonomic studies.Sociobiology. 19:51–76.
Haverty, M.I.,Nelson, L.J.,Thorne, B.L.,Collins, M.S.,Darlington, J., andPage, M. Cuticular hydrocarbons for species determination of tropical termites. Submitted.
Henderson, G., Andersen, J.F., Phillips, J.K. andJeanne, R.L. 1990. Internest aggression and identification of possible nestmate discrimination pheromones in polygynous antFormica montana.J. Chem. Ecol. 16:2217–2228.
Hölldobler, B., andCarlin, N.F. 1987. Anonymity and specificity in the chemical communication signals of social insects.J. Comp. Physiol. 161A:567–581.
Hoppes, K.L., Dillwith, J.W., Wright, R.E., andSzumlas, D.E. 1990. Identification of horse flies (Diptera: Tabanidae) by analysis of cuticular hydrocarbons.J. Med. Entomol. 27:481–486.
Howard, R.W., McDaniel, C.A., Nelson, D.R., andBlomquist, G.J. 1980a. Chemical ionization mass spectrometry: Application to insect-derived cuticular alkanes.J. Chem. Ecol., 6:609–623.
Howard, R.W., andBlomquist, G.J. 1982. Chemical ecology and biochemistry of insect hydrocarbons.Annu. Rev. Entomol. 27:149–172.
Howard, R.W., McDaniel, C.A., andBlomquist, G.J. 1978. Cuticular hydrocarbons of the eastern subterranean termite,Reticulitermes flavipes (Kollar) (Isoptera: Rhinotermitidae).J. Chem. Ecol. 4:233–245.
Howard, R.W., McDaniel, C.A., andBlomquist, G.J. 1980b. Chemical mimicry as an integrating mechanism: Cuticular hydrocarbons of a termitophile and its host.Science 210:431–433.
Howard, R.W., McDaniel, C.A., andBlomquist, G.J. 1982a. Chemical mimicry as an integrating mechanism for three termitophiles associated withReticulitermes virginicus.Psyche 89:157–167.
Howard, R.W., McDaniel, C.A., Nelson, D.R., Blomquist, G.J., Gelbaum, andZalkow, L.H. 1982b. Cuticular hydrocarbons ofReticulitermes virginicus (Bank) and their role as potential species- and caste-recognition cues.J. Chem. Ecol. 8:1227–1239.
Howard, R.W., Thorne, B.L., Levings, S.C., andMcDaniel, C.A. 1988. Cuticular hydrocarbons as chemotaxonomic characters forNasutitermes corniger (Motschulsky) andN. ephratae (Holmgren) (Isoptera: Termitidae).Ann. Entomol. Soc. Am. 81:395–399.
Howard, R.W., Akre, R.D., andGarnett, W.B. 1990a. Chemical mimicry in an obligate predator of carpenter ants (Hymenoptera: Formicidae).Ann. Entomol. Soc. Am. 83:607–616.
Howard, R.W., Stanley-Samuelson, D.W., andAkre, R.D. 1990b. Biosynthesis and chemical mimicry of cuticular hydrocarbons from the obligate predator,Microdon albicomatus Novak (Diptera: Syrphidae) and its ant prey,Myrmica incompleta Provencher (Hymenoptera: Formicidae).J. Kansas Entomol. Soc. 63:437–443.
Howse, P.E. 1975. Chemical defences of ants, termites and other insects, some outstanding questions, in pheromones and defensive secretions in social insects. Symposium of the I.U.S.S.I., Dijon, pp. 23–40.
Jaffe, K. 1980. Chemical communication systems in the antAtta cephalotes, in Social Insects on the Tropics, Vol. 2, pp. 165–180.
Killian, A. 1989. Etude Comportementale de l'agression interspécifique desReticulitermes (Isoptères). Mémoire de Diplome d'Etude Approfondie. 51 pp.
Lange, C., Basselier, J.J., Bagnères, A.G., Escoubas, P., Lemaire, M., Lenoir, A., Clement, J.L., Bonavita-Cougourdan, A., Trabalon, M., andCampan, M. 1989. Strategy for the analysis of cuticular hydrocarbons waxes from insects using gas chromatography/mass spectrometry with electron impact and chemical ionization.Biomed. Env. Mass Spec. 18:787–800.
Lockey, K.H. 1988. Lipids of the insect cuticule: Origin, composition and function.Comp. Biochem. Physiol. 89B:595–645.
Lockey, K.H. 1991. Cuticular hydrocarbons of adultOnymacris bicolor (Haag) andOnymacris boschimana (Péringuey) (Coleoptera: Tenebrionidae).Comp. Biochem. Physiol. 98B:151–163.
Lockey, K.H. andMetcalfe, N.B. 1988. Cuticular hydrocarbons of adultHimatismus species and a comparison with 21 other species of adult tenebrionid beetle using multivariate analysis.Comp. Biochem. Physiol. 91B:371–382.
Luytens, I. 1982. Variations intraspécifiques et interspécifiques des hydrocarbures cuticulaires chezDrosophila simulons et des espèces affines.C.R. Acad. Sci. 295:733–736.
Morel, L., Vander Meer, R.K., andLavine, B.K. 1988. Ontogeny of nestmate recognition cues in the red carpenter ant (Camponotus floridanus).Behav. Ecol. Sociobiol. 22:175–183.
Nel, J.J.C. 1968. Aggressive behaviour of the harvester termitesHodotermes mossambicus (Hagen) andTrinervitermes trinervoides (Sjöstedt).Ins. Soc. 15:145–156.
Nelson, D.R., andCarlson, D.A. 1986. Cuticular hydrocarbons of the tsetse fliesGlossina morsitans morsitans, G. austeni andG. pallidipes.Insect Biochem. 16:403–416.
Nelson, D.R., Carlson, D.A., andFatland, C.L. 1988. Cuticular hydrocarbons of tsetse flies II:Glossina fuscipes fuscipes, G. palpalis palpalis, G. p. gambiensis, G. tachinoides, andG. brevipaldis.J. Chem. Ecol. 14:963–987.
Nowbahari, E., Lenoir, A., Clément, J.L., Lange, C., Bagnères, A.G., andJoulie, C. 1990. Individual, geographical and experimental variation of cuticular hydrocarbons of the antCataglyphis cursor (Hymenoptera: Formicidae): Their use in nest and subspecies recognition.Biochem. Syst. Ecol. 18:63–73.
Obin, M.S. 1986. Nestmate recognition cues in laboratory and field colonies ofSolenopsis invicta Buren (Hymenoptera: Formicidae): Effect of environment and role of cuticular hydrocarbons.J. Chem. Ecol. 12:1965–1975.
Page, M., Nelson, L.J., Haverty, M.I., andBlomquist, G.J. 1990. Cuticular hydrocarbons of eight species of North American cone beetles,Conophthorus Hopkins.J. Chem. Ecol. 16:1173–1198.
Plattner, R.D., Spencer, G.F., andKleinan, R. 1976. Double bond location in polyenoic fatty esters through partial oxymercuration.Lipids 11:222–227.
Pomonis, J.G., Hakk, H., andFatland, C.L. 1989. Synthetic methyl- and dimethylalkanes: Kovats Indices, [13C]NMR and mass spectra of some methylpentacosanes and 2,X-dimethyl-heptacosanes.J. Chem. Ecol. 15:2319–2333.
Ross, K.G., Vander Meer, R.K., Fletcher, D.J.C., andVargo, E.J. 1987. Biochemical phenotypic and genetic studies of two introduced fire ants and their hybrid (Hymenoptera: Formicidae).Evolution 41:280–293.
Springhetti, A., andAmorelli, M. 1982. Competitive behavior between two species of Isoptera:Kalotermes flavicolli (Kalotermitidae) andReticulitermes lucifugus (Rhinotermitidae).Sociobiology 7:155–164.
Thorne, B.L. 1982. Termite-termite interactions: Workers as an agonistic caste.Psyche 89:133–150.
Vander Meer, R.K., Lofgren, C.S., andAlvarez, F.M. 1985. Biochemical evidence for hybridization in fire ants.Florida Entomol. 68:501–506.
Wigglesworth, V.B. 1933. The physiology of the cuticule and of ecdysis inRhodnius prolixus (Triatomodae, Hemiptera); with special reference to the function of the oenocytes and of the dermal glands.Quart. J. Microsc. Sci. 76:269–318.
Wigglesworth, V.B. 1990. The Distribution, function and nature of “cuticulin” in the insect cuticule.J. Ins. Physiol. 36:307–313.
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Bagneres, AG., Killian, A., Clement, JL. et al. Interspecific recognition among termites of the genusReticulitermes: Evidence for a role for the cuticular hydrocarbons. J Chem Ecol 17, 2397–2420 (1991). https://doi.org/10.1007/BF00994590
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DOI: https://doi.org/10.1007/BF00994590