Abstract
Florida queen butterflies are highly variable in cardenolide content and, in three populations studied, contained less cardenolide than did a sample of sympatric Florida monarchs. The possibility that queens stored a more potent set of cardenolides from their host plants (and therefore were as well protected as monarchs, even at lower concentrations) is refuted by Chromatographic analysis of wild butterflies, as well as controlled laboratory rearings. It therefore appears that, with respect to cardenolides, monarchs are better defended than are queens. Consequently, cardenolides are unlikely to explain the apparent shift in Florida viceroy mimicry away from resemblance of the monarch, toward mimicry of the queen. Other hypotheses to explain this mimetic phenomenon are suggested. Adult monarchs exhibit significant negative correlations between the concentration of cardenolide stored in their tissues and both body size and weight, whereas queens show no such correlations. The implications of these results for the study of “metabolic costs” of allelochemic storage are discussed. Chromatographic evidence is provided that monarchs do breed in south Florida during the winter months and that the likely host plant employed by the population studied wasAsclepias curassavica. This represents the first practical application of cardenolide “fingerprinting” to identify the larval host plants of wild danaid butterflies.
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References
Boppré, M. 1978. Chemical communication, plant relationships, and mimicry in the evolution of danaid butterflies.Entomol. Exp. appl. 24:264–277.
Brower, J.V.Z. 1958a. Experimental studies of mimicry in some North American butterflies. I. The monarchDanaus plexippus and viceroyLimenitis archippus.Evolution 11:32–47.
Brower, J.V.Z. 1958b. Experimental studies of mimicry in some North American butterflies. III.Danaus gilippus berenice andLimenitis archippus floridemis.Evolution 12:273–285.
Brower, L.P. 1961. Studies on the migration of the monarch butterfly. I. Breeding populations ofDanaus plexippus andD. gilippus berenice in south central Florida.Ecology 42:76–83.
Brower, L.P. 1969. Ecological chemistry.Sci. Am. 220:22–29.
Brower, L.P. 1984. Chemical defence in butterflies.Symp. R. Entomol. Soc. 11:109–134.
Brower, L.P., andBrower, J.V.Z. 1964. Birds, butterflies, and plant poisons: A study in ecological chemistry.Zoologica 49:137–159.
Brower, L.P., andGlazier, S.C. 1975. Localization of heart poisons in the monarch butterfly.Science 188:19–25.
Brower, L.P., andMoffitt, C.M. 1974. Palatability dynamics of cardenolides in the monarch butterfly.Nature 249:280–283.
Brower, L.P., Ryerson, W.N., Coppinger, L.L., andGlazier, S.C. 1968. Ecological chemistry and the palatability spectrum.Science 161:1349–1351.
Brower, L.P., McEvoy, P.B., Williamson, K.L., andFlannery, M.A. 1972. Variation in cardiac glycoside content of monarch butterflies from natural populations in eastern North America.Science 177:426–429.
Brower, L.P., Edmunds, M., andMoffitt, C.M. 1975. Cardenolide content and palatability of a populationof Danaus chrysippus butterflies from West Africa.J. Entomol. (A) 49:183–196.
Brower, L.P., Gibson, D.O., Moffitt, C.M., andPanchen, A.L. 1978. Cardenolide content ofDanaus chrysippus butterflies from three regions of East Africa.Biol. J. Linn. Soc. 10:251–273.
Brower, L.P., Seiber, J.N., Nelson, C.J., Lynch, S.P., andTuskes, P.M. 1982. Plant-determined variation in the Cardenolide content, thin-layer chromatography profiles, and emetic potency of monarch butterflies,Danaus plexippus, reared on the milkweed,Asclepias eriocarpa in California.J. Chem. Ecol. 8:579–633.
Brower, L.P., Seiber, J.N., Nelson, C.J., Lynch, S.P., andHolland, M. 1984a. Plant-determined variation in the cardenolide content, thin-layer chromatography profiles, and emetic potency of monarch butterflies,Danaus plexippus L., reared on milkweek plants in California: 2.Asclepias speciosa.J. Chem. Ecol. 10:601–639.
Brower, L.P., Seiber, J.N., Nelson, C.J., Lynch, S.P., Hoggard, M.P., andCohen, J.A. 1984b. Plant-determined variation in cardenolide content and thin-layer chromatography profiles of monarch butterflies,Danaus plexippus, reared on the milkweed,Asclepias californica in California.J. Chem. Ecol. 10:1823–1857.
Burns, J.M. 1983. Queen of the Carolinas (Lepidoptera: Nymphalidae: Danainae:Danaus gilippus).Proc. Entomol. Soc. Wash. 85:388–396.
Cohen, J.A. 1983. Chemical interactions among milkweed plants (Asclepiadaceae) and lepidopteran herbivores. PhD dissertation. University of Florida, Gainesville.
Cohen, J.A., andBrower, L.P. 1982. Oviposition and larval success of wild monarch butterflies (Lepidoptera: Danaidae) in relation to hostplant size and cardenolide content.J. Kans. Entomol. Soc. 55:343–348.
Cohen, J.A., andBrower, L.P. 1983. Cardenolide sequestration by'the dogbane tiger moth (Cycnia tenera; Arctiidae).J. Chem. Ecol. 9:521–532.
Dixon, C.A., Erickson, J.M., Kellett, D.N., andRothschild, M. 1978. Some adaptations betweenDanaus plexippus and its food plant, with notes onDanaus chrysippus andEuploea core (Insecta: Lepidoptera).J. Zool. London. 185:437–467.
Edgar, J.A. 1975. Danainae (Lep.) and 1,2-dehydropyrrolizidine alkaloid-containing plants—with reference to observations made in the New Hebrides.Phil. Trans. R. Soc. (B) 272:467–476.
Edgar, J.A., Cockrum, P.A., andFrahn, J.L. 1976. Pyrrolizidine alkaloids inDanaus plexippus L. andDanaus chrysippus L.Experientia 32:1535–1537.
Edgar, J.A., Boppré, M., andSchneider, D. 1979. Pyrrolizidine alkaloid storage in African and Australian danaid butterflies.Experientia 35:1447–1448.
Ehrlich, P.R., andRaven, P.H. 1964. Butterflies and plants: A study in coevolution.Evolution 18:586–608.
Erickson, J.M. 1973. The utilization of variousAsclepias species by larvae of the monarch butterfly,Danaus plexippus.Psyche 80:230–244.
Helwig, J.T., andCouncil, K.A. 1979. SAS user's guide. SAS Institute Inc., Gary, North Carolina.
Hinton, H.E. 1981. Biology of Insect Eggs, Vol. 1. Pergamon Press, Oxford.
Klots, A.B. 1951. A Field Guide to the Butterflies. Houghton-Mifflin, New York.
Marty, M.A. 1983. Metabolism and disposition of milkweed (Asclepiadaceae) cardenolides in monarch butterfly larvae,Danaus plexippus, and in a predator,Peromyscus maniculatus. PhD dissertation. University of California, Davis.
Meinwald, J., Meinwald, Y.C., andMazzocchi, P.H. 1969. Sex pheromone of the queen butterfly: Chemistry.Science 164:1174–1175.
Nelson, C.J., Seiber, J.N., andBrower, L.P. 1981. Seasonal and intraplant variation of cardenolide content in the California milkweed,Asclepias eriocarpa, and implications for plant defense.J. Chem. Ecol. 7:981–1010.
Nishio, S. 1980. The fates and adaptive significance of cardenolides sequestered by larvaeof Danaus plexippus (L.) andCycnia inopinatus (Hy. Edwards). PhD dissertation. University of Georgia, Athens.
Pliske, T.E., andEisner, T. 1969. Sex pheromone of the queen butterfly: Biology.Science 164:1170–1172.
Pough, F.H., Brower, L.P., Meck, H.R., andKessell, S.R. 1973. Theoretical investigations of automimicry: Multiple trial learning and the palatability spectrum.Proc. Natl. Acad. Sci. U. S.A. 70:2261–2265.
Reichstein, T., von Euw, J., Parsons, J.A., andRothschild, M. 1968. Heart poisons in the monarch butterfly.Science 161:861–866.
Remington, C.L. 1968. Suture zones of hybrid interaction between recently joined biotas, pp. 321–428,in T. Dobzhansky, M.K. Hecht, and W.C. Steere (eds.). Evolutionary Biology, Vol. 2. Appleton-Century-Crofts, New York.
Roeske, C.N., Seiber, J.N., Brower, L.P., andMoffitt, C.M. 1976. Milkweed cardenolides and their comparative processing by monarch butterflies (Danaus plexippus L.).Rec. Adv. Phytochem. 10:93–167.
Rothschild, M., andMarsh, N. 1978. Some peculiar aspects of Danaid/plant relationships.Entomol. Exp. Appl. 24:437–450.
Rothschild, M., von Euw, J., Reichstein, T., Smith, D.A.S., andPierre, J. 1975. Cardenolide storage inDanaus chrysippus (L.) with additional notes onD. plexippus (L.).Proc. R. Soc. London. (B) 190:1–31.
Seiber, J.N., Tuskes, P.M., Brower, L.P., andNelson, C.J. 1980. Pharmacodynamics of some individual milkweed cardenolides fed to larvae of the monarch butterfly (Danaus plexippus L.).J. Chem. Ecol. 6:321–339.
Tietz, H.M. 1972. An index to the described life histories, early stages, and hosts of the Macrolepidoptera of the continental United States and Canada. A.C. Allyn Museum of Entomology, Sarasota, Fla.
Urquhart, F.A. 1960. The Monarch Butterfly. University of Toronto Press, Toronto.
Urquhart, F.A., andUrquhart, N.R. 1976. A study of the peninsular Florida populations of the monarch butterfly (Danaus p. plexippus; Danaidae).J. Lepidop. Soc. 30:73–87.
Urquhart, F.A., andUrquhart, N.R. 1977. The overwintering site of the eastern population of the monarch butterfly (Danaus plexippus; Danaidae) in southern Mexico.J. Lepidop. Soc. 30:153–158.
Walford, P., andBrower, L.P. 1985. Lipids in the migration cycle of the monarch butterfly,Danaus plexippus. In preparation.
Woodson, R.E. 1954. The North American species ofAsclepias L.Ann. Missouri Bot. Gard. 41:1–261.
Young, A.M. 1982. An evolutionary-ecological model of the evolution of migratory behavior in the monarch butterfly, and its absence in the queen butterfly.Acta Biotheor. 31:219–237.
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Cohen, J.A. Differences and similarities in cardenolide contents of queen and monarch butterflies in florida and their ecological and evolutionary implications. J Chem Ecol 11, 85–103 (1985). https://doi.org/10.1007/BF00987608
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DOI: https://doi.org/10.1007/BF00987608