Abstract
The recent development of molecular marker technology is revolutionising the study of plant populations, providing opportunities to address questions requiring a precise knowledge of pedigrees. We applied Inter-Simple Sequence Repeat (ISSR) PCR to severalBrassica oleracea accessions and toBrassica napus. Four microsatellite-primers were screened and, among the 136 reproducible fragments recorded, 25 (18.4%) fragments were common for allBrassica, 27 (19.9%) were unique and 84 (61.7%) were phylogenetically informative. Each individual test sample exhibited a unique molecular genotype. ISSR markers provided a rapid approach to analyse genetic diversity and reflected the known genetic relationships among selected entries. ISSR markers appeared of great value in gene bank management and the establishment of genetic similarity, and can be applied to allogamous (autumn and winter cauliflower) crops.
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Leroy, X.J., Leon, K. & Branchard, M. Characterisation ofBrassica oleracea L. by microsatellite primers. Pl Syst Evol 225, 235–240 (2000). https://doi.org/10.1007/BF00985470
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DOI: https://doi.org/10.1007/BF00985470