Abstract
The concentrations of taurine in blood and brain regions of the toadBufo boreas have been measured. Most of these values are considerably lower than those found in mammals. Using an antibody prepared against conjugated taurine, the distribution of taurine in three brain regions of the toad has been visualized. The possible osmoregulatory functions of taurine have been investigated by making toads hyper- or hypo-osmotic in vivo. Induction of hypoosmolality is accompanied by a massive taurine tide in blood plasma, but has no immediate effects upon the taurine concentrations in the brain areas studied. However, histochemical visualization indicates a marked redistribution of taurine between cellular components and extracellular space of brain tissues. This may indicate that taurine has an osmoregulatory function in brain tissue under hypo-osmotic conditions. Hyperosmolality results in no elevation of the taurine concentration in blood plasma of toads, but rather in a very gradual decline of total plasma taurine content over a prolonged time period. Histochemical studies reveal little change in frontal cortex after 1 hour but deeper staining of many neurons in optic lobe accompanied by greater staining in the extracellular fluid. By 3 hours there is a depletion of taurine from all compartments of cerebral cortex tissues. No evidence of any prolonged direct osmoregulatory role for taurine is indicated under hyperosmotic conditions. A possible indirect osmoregulatory function of taurine is discussed.
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Gordon, M. S. 1985. Intracellular osmoregulation in skeletal muscle during salinity adaptation in two species of toads. Biol. Bull. 12:218–229.
Baxter, C. F., and Baldwin, R. A. 1978. A functional role for amino acids in the adaptation of tissues from the nervous system to alternations in environmental osmolality. Pages 599–625,in Fonnum, F. (ed.), Amino Acids as Chemical Transmitters, Plenum Publishing Corp., NY.
Baxter, C. F., Wasterlain, C. G., Hallden, K. L., and Pruess, S. F. 1986. Effect of altered blood plasma osmolalities on regional brain amino acid concentrations and focal seizure susceptibility in the rat. J. Neurochem. 47:617–624.
Chan, P. H., and Fishman, R. A. 1979. Elevation of rat brain amino acids, ammonia and idiogenic osmoles induced by hyperosmolality. Brain Res. 161:293–301.
Puka, M., Sundell, K., Lazarewicz, J. W., and Lehmann, A. 1991. Species differences in cerebral taurine concentrations correlate with brain water content. Brain Res. 548:267–272.
Baxter, C. F., Baldwin, R. A., and Oh, C. C. 1990. Acclimation to hyper and hypoosmotic changes: The anomalous behavior of taurine in the nervous system and in the blood of vertebrates. Pages 329–336,in Pasantes-Morales, H., Martin, D. L., Shain, W., and Del Rio, R. M. (eds.), Taurine, Functional Neurochemistry, Physiology and Cardiology, Wiley-Liss, John Wiley and Sons, Inc., NY.
Thurston, J. H., Hauhart, R. E., and Dirgo, J. A. 1980. Taurine: A role in osmotic regulation of mammalian brain and possible clinical significance. Life Sci. 26:1561–1568.
Law, R. O. 1989. Effects of pregnancy on contents of water, taurine and total amino nitrogen in rat cerebral cortex. J. Neurochem. 53:300–302.
Trachtman, H., Barbour, R., Sturman, J. A., and Finberg, L. 1988. Taurine and osmoregulation: Taurine is a cerebral osmoprotective molecule in chronic hypernatremic dehydration. Ped. Res. 23:35–39.
Pasantes-Morales, H., and Schousboe, A. 1988. Volume regulation in astrocytes: A role for taurine as an osmoeffector. J. Neurosci. Res. 20:505–509.
Sanchez-Olea, R., Moran, J., and Pasantes-Morales, H. 1992. Changes in taurine transport evoked by hyperosmolarity in cultured astrocytes. J. Neurosci. Res. 32:86–92.
Wade, J. V., Olson, J. P., Samson, F. E., Nelson, S. R., and Pazdernik, T. L. 1988. A possible role for taurine in osmoregulation within the brain. J. Neurochem. 51:740–745.
Huxtable, R. J. 1989. Taurine in the central nervous system and the mammalian actions of taurine. Progr. Neurobiol. 32:471–533.
Okamoto, K., Kimura, H., and Sakai, Y. 1983. Evidence for taurine as an inhibitory neurotransmitter in cerebellar stellate interneurons: selective antagonism by TAG (6-aminomethyl-3-methyl-4H,1,2,4-benzothiadiazine-1,1-dioxide). Brain Res. 265:163–168.
Sturman, J. A. 1986. Nutritional taurine and central nervous system development.In Mental Retardation: Research, Education, and Technology Transfer, Ann. NY Acad. Sci. 477:196–213.
Palackal, T., Moretz, R. C., Wisniewski, H. M., and Sturman, J. A. 1988. Ultrastructural abnormalities in the visual cortex of kittens from taurine-deficient mothers. Brain Dysfunc. 1:71–89.
van Gelder, N. M. 1983. A central mechanism of action for taurine: Osmoregulation, bivalent cations and excitation threshold. Neurochem. Res. 8:687–699.
van Gelder, N. M. 1989. Brain taurine content as a function of cerebral metabolic rate: Osmotic regulation of glucose derived water production. Neurochem. Res. 14:495–497.
Storm-Mathisen, J., Leknes, A. K., Bore, A. T., Vaaland, J. L., Edminson, P., Haug, F. S., and Ottersen, O. P. 1983. First visualization of glutamate and GABA in neurones by immunocytochemistry. Nature 301:517–520.
Ottersen, O. P., and Storm-Mathisen, J. 1984. Glutamate- and GABA-containing neurons in the mouse and rat brain, as demonstrated with a new immunocytochemical technique. J. Comp. Neurol. 229:374–392.
Ottersen, O. P., and Storm-Mathisen, J. 1987. Localization of amino acid neurotransmitters by immunocytochemistry. TINS 6:250–255.
Campistron, G., Geffard, M., and Buijs, R. M. 1986. Immunological approach to the detection of taurine and immunocytochemical results. J. Neurochem. 46:862–868.
Magnusson, K. R., Madl, J. E., Clements, J. R., Wu, J.-Y., Larson, A. A., and Beitz, A. J. 1988. Colocalization of taurine-and cysteine sulfinic acid decarboxylase-like immunoreactivity in the cerebellum of the rat with monoclonal antibodies against taurine. J. Neurosci. 8:4551–4564.
Clements, J. R., Magnusson, K. R., and Beitz, A. J. 1990. Ultrastructural description of glutatmate-, aspartate-, taurine-, and glycine-like immunoreactive terminals from five rat brain regions. J. Elec. Microscopy Tech. 15:49–66.
Rose, B. B., Baxter, C. F., Dole, J. W., Tachiki, K. H., and Baldwin, R. A. 1986. Abnormal feeding behavior of Western toads (Bufo boreas) kept in a hyperosmotic environment. 1. A quantitative behavioral analysis as related to cerebral amino-acid cencentrations. Pharmacol. Biochem. Behav. 24:1315–1321.
Tachiki, K. H., and Baxter, C. F. 1979. Taurine levels in brain tissues: A need for re-evaluation. J. Neurochem. 33:1125–1129.
Braswell, E. 1971. Resolution of overlapping Gaussian curves by means of a simple digital computer program. Analyt. Biochem. 44:58–65.
Frank, H. A., and Carr, M. H. 1955. A simplified dye method for estimating plasma volume. J. Lab. Clin. Med. 45:977–980.
Baxter, C. F. 1968. Intrinsic amino acid levels and the blood-brain barrier. Pages 429–446,in Lajtha, A., and Ford, D. H. (eds.), Brain Barrier Systems, Elsevier Publishing Co., Amsterdam.
Westcor, 1987. Westcor Inc., Service Manual M2448-2 Logan, Utah 84321.
Lake, N., and Verdone-Smith, C. 1989. Immunocytochemical localization of taurine in the mammalian retina. Current Eye Res. 8:163–173.
Lu, P., Schuller-Levis, G., and Sturman, J. A. 1991. Distribution of taurine-like immunoreactivity in cerebellum of kittens from taurine-supplemented and taurine-deficient mothers. Int. J. Devl. Neurosci. 9:621–629.
Sturman, J. A., and Gaull, G. E. 1975. Taurine in the brain and liver of developing human and monkey. J. Neurochem. 25:831–835.
Sturman, J. A., and Hayes, K. C. 1980. The biology of taurine in nutrition and development. Pages 231–299,in Draper, H. H. (ed.), Advances Nutritional Research, Plenum Publishing Corp., NY.
Palkovits, M., Elekes, I., Lang, T., and Patthy, A. 1986. Taurine levels in discrete brain nuclei of rats. J. Neurochem. 47:1333–1335.
Hayes, K. C., and Sturman, J. A. 1981. Taurine in metabolism. Ann. Rev. Nutr. 1:401–425.
Baxter, C. F., Baldwin, R. A., and Oh, C. C. 1989. Does taurine have an osmoregulatory function in tissues of the nervous system? J. Neurochem. 52:S102.
Florkin, M., and Schoffeniels, E. 1965. Euryhalinity and the concept of physiological radiation. Pages 6–40,in Munday, K. A. (ed.), Studies in Comparative Biochemistry, Pergamon Press, NY.
Gilles, R., and Gerard, J. F. 1974. Amino acid metabolism during osmotic stress in isolated axons ofCallinectes sapidus. Life Sci. 14:1221–1229.
Simpson, J. W., Allen, K., and Awapara, J. 1959. Free amino acids in some aquatic invertebrates. Biol. Bull. 117:371–381.
Huxtable, R., and Barbeau A., eds. 1976. Taurine. 398 Pages, Raven Press, NY.
Pasantes-Morales, H., Martin, D. L., Shain, W., and Martin del Rio, R., eds. 498 pages, Taurine: Functional Neurochemistry, Physiology and Cardiology, Wiley-Liss Inc., NY 1990.
Lombardini, J. B., Schaffer, S. W., and Azuma, J., eds. 456 pages, Taurine: Nutritional Value and Mechanisms of Action, Advances in Experimental Medicine and Biology, Plenum Press, NY 1992.
Lehmann, A., Carlstrom, C., Nagelhus, E. A., and Ottersen, O. P. 1991. Elevation of taurine in hippocampal extracellular fluid and cerebrospinal fluid of acutely hypoosmotic rats: Contribution by influx from blood? J. Neurochem. 56:690–697.
Kulakowski, E. C., Maturo, J., Schaffer, S. W. 1985. The low affinity taurine-binding protein may be related to the insulin receptor. Pages 127–136,in Oja, S. S., Ahtee, L., Kontro, P., and Passonen, M. K. (eds.), Taurine: Biological Action and Clinical Perspectives, Alan P. Liss, NY.
Baxter, C. F. 1986. The effect of altered osmolalities upon putative neurotransmitter amino acids in the central nervous system: Biochemical mechanisms with some physiological and behavioral correlates. Bull. Tokyo Metropol. Inst. for Neurosci. 15: Suppl. 2:83–99.
Shank, R. P., and Baxter, C. F. 1973. Metabolism of glucose, amino acids, and some related metabolites in the brain of the toads (Bufo boreas) adapted to fresh water or hyperosmotic environments. J. Neurochem. 21:301–313.
Schousboe, A., Sanchez Olea, R., Moran, J., and Pasantes-Morales, H. 1991. Hyposmolarity-induced taurine release in cerebellar granule cells is associated with diffusion and not with high-affinity transport. J. Neurosci. Res. 30:662–665.
Kimelberg, H. K., Goderie, S. K., Higman, S., Pang, S., and Waniewski, R. A. 1990. Swelling-induced release of glutamate, aspartate and taurine from astrocyte cultures. J. Neurosci. 10:1583–1591.
Walz, W., and Allen, A. F. 1987. Evaluation of the osmoregulatory function of taurine in brain cells. Exp. Brain Res. 68:290–298.
Holopainen, I., Malminen, O., and Kontro, P. 1987. Sodium-dependent high affinity uptake of taurine in cultured cerebellar granule cells and astrocytes. J. Neurochem. Res. 18:479–483.
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Special issue dedicated to Dr. Claude Baxter.
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Baxter, C.F., Baldwin, R.A., Lu, P. et al. Taurine in toad brain and blood under different conditions of osmolality: An immunohistochemical study. Neurochem Res 18, 425–435 (1993). https://doi.org/10.1007/BF00967246
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DOI: https://doi.org/10.1007/BF00967246