Abstract
Developmental changes in monoamine oxidase (MAO) activity in the mouse brain were investigated with the substrates β-phenylethylamine (PEA), tryptamine, and 5-hydroxytryptamine (5-HT). In the newborn brain, MAO activity towards PEA was found to be much lower than the adult and to be inhibited by clorgyline in a double-sigmoidal fashion. The inhibition curve shifted to a single-sigmoidal pattern with age. MAO activity towards 5-HT as substrate was inhibited by 90% and in a single-sigmoidal manner by clorgyline throughout the postnatal life. Lineweaver-Burk plots with PEA as substrate presented two linear lines (apparentK m: 28.6 and 4.1 μM) for the newborn and one line (apparentK m: 11.4 μM) for the adult, respectively. The plot with highK m value for the newborn brain disappeared in a clorgyline-treated preparation. These findings suggest that age-dependent alterations in the ratio of MAO-A/MAO-B activity affect the substrate specificity of the enzyme.
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Koide, Y., Kobayashi, K. Developmental changes in the activity and substrate specificities of mouse brain monoamine oxidase. Neurochem Res 9, 595–606 (1984). https://doi.org/10.1007/BF00964506
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DOI: https://doi.org/10.1007/BF00964506