Abstract
Brain ischemia was produced in gerbils (Meriones unguiculatus) by the bilateral ligation of the carotid arteries with reported procedures. Changes in the energy status of brain demonstrated that carotid ligation was effective. At different time intervals from ligation, groups of ferbils were given either saline or S-Adenosyl-l-methionine (SAMe) by the intraventricular (i.v.) route (1.6 mg/Kg body wt. twice, at each 10 min interval), or by the intraperitoneal (i.p.) administration (200 mg/Kg body wt.) or subcutaneously (s.c.) with 40 mg/Kg body wt, daily, for two weeks. Control animals, with and without SAMe, together with the ischemic groups, were decapitated directly into liquid nitrogen, 10 min after ligation. Brain neutral and polar lipid, together with free fatty acids, which were all labeled in vivo by the intraventricular injection of [1-14C]arachidonic acid 2 hr prior to ligation, were extracted, purified and separated by conventional procedures. SAMe when injected i.v. or i.p. noticeably corrected the changes in polar lipid by reversing the decrease of brain phosphatidylcholine and choline plasmalogen, as well as of their labeling, which was due to ischemia. Concurrently with this action, SAMe treatment (i.v. and i.p.) also provided to some extent to re-establish the normal level of labeling of ethanolamine lipids. When SAMe was given s.c., no effect was present. SAMe had no effect on the increase of free fatty acid and diglyceride due to ischemia. The prevention by SAMe of the changes of choline lipids suggests that a stimulation of the methyltransferase reaction may occur in the ischemic brain, due to increased substrate (SAMe) availability. This effect may be important for cell survival, since membrane phospholipid derangements alter the properties of the membrane.
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Mozzi, R., Siepi, D., Aandreoli, V., andPorcellati, G. 1982. Conversion of phosphatidylethanolamine to phosphatidylcholine by the methylation pathway in brain, and relation with the membrane structure. Pages. 129–138,in Usdin, E., Borchardt, T. T., andCreveling, C. R. (eds.), Biochemistry of S-Adenosylmethionine and Related Compounds, McMillan Press, New York.
Baldessarini, R. J., andKopin, I. J. 1966. S-Adenosylmethionine in brain and other tissues. J. Neurochem. 13:769–777.
Schatz, R. A., Stramentinoli, G., andSellinger, O. Z. 1981. Decreased cerebral catabolism of [3H] Histamine in Vivo after S-Adenosylmethionine administration. J. Pharmacol. Exptl. Therap. 216:118–124.
Stramentinoli, G., Catto, E., andAlgeri, S. 1977. The increase in S-Adenosylmethionine (SAMe) concentration in rat brain after its systemic administration. Comm. Psychopharmacol. 1:89–97.
Mozzi, R., andPorcellati, G. 1979. Conversion of phosphatidylethanolamine to phosphatidylcholine in rat brain by the methylation pathway. FEBS Letters 100:363–366.
Blusztajn, J. K., Zeisel, S. H., andWurtman, R. J. 1979. Synthesis of lecithin (phosphatidylcholine) from phosphatidylethanolamine in bovine brain. Brain Res. 179:319–327.
Crews, F. T., Hirata, F., andAxelrod, J. 1980. Identification and properties of methyltransferases that synthesize phosphatidylcholine in rat brain synaptosomes. J. Neurochem. 35:1491–1498.
Mozzi, R., Siepi, D., Andreoli, V., andPorcellati, G. 1981. The synthesis of choline plasmalogen by the methylation pathway in rat brain. FEBS Letters 131:115–118.
Mozzi, R., Goracci, R., Siepi, D., Francescangeli, E., Andreoli, V., Horrocks, L. A., andPorcellati, G. 1982. Phospholipid synthesis by interconversion reactions in brain tissue. Pages 1–12,in Horrocks, L. A., Ansell, G. B., andPorcellati, G. (eds.), Phospholipids in the Nervous System. Vol. I, Raven Press, New York.
Elioranta, T. O. 1977. Tissue distribution of S-Adenosyl-methionine and S-Adenosylhomocysteine in the rat. Effect of age, sex and methionine administration on the metabolism of S-Adenosyl-methionine, S-Adenosyl-homocysteine and polyamines. Biochem. J. 166:521–529.
Mozzi, R., Andreoli, V., andPorcellati, G. 1980. Involvement of S-adenosylmethionine in brain phospholipid metabolism. Pages 41–54,in Cavallini, D., Gaull, G. E., andZappia, V. (eds.), Natural Sulfur Compounds: Novel Biochemical and Structural Aspects, Plenum Press, New York.
Calderini, G., Aldinio, C., Crews, F. T., Gaiti, A., Scapagnini, U., Algeri, S., Ponzio, F., andToffano, G. 1981. Aging and information processing. Pages 54–56,in Corsini, G. U., andGessa, G. L. (eds.), Apomorphine and Other Dopaminomimetics. Vol. 2, Raven Press, New York.
Trovarelli, G., De Medio, G. E., Dorman, R. V., Piccinin, G. L., Horrocks, L. A., andPorcellati, G. 1981. Effect of cytidine-diphosphate choline (CDP-choline) on ischemia-induced alterations of brain lipid in gerbil. Neurochem. Res. 6:821–833.
Siesjö, B. K. 1978. Brain Energy Metabolism, pages 458–566. John Wiley and Sons, New York.
De Medio, G. E., Goracci, G., Horrocks, L. A., Lazarewicz, J. W., Mazzari, S., Porcellati, G., Strosznajder, J., andTrovarelli, G. 1980. The effect of transient ischemia on fatty acid and lipid metabolism in the gerbil brain. Ital. J. Biochem. 29:412–432.
Porcellati, G., De Medio, G. E., Fini, C., Floridi, A., Goracci, G., Horrocks, L. A., Lazarewicz, J. W., Palmerini, C. A., Strosznajder, J., andTrovarelli, G. 1978. Phospholipid and its metabolism in ischemia. Pages 285–302,in Neuhoff, V. (ed.), Proceedings of the European Society for Neurochemistry, Vol. I, Verlag Chemie, Weinheim.
Bazan, N. G., Jr., Bazan, H. E. P., Kennedy, W. G., andJoel, C. D. 1971. Regional distribution and rate of production of free fatty acids in rat brain. J. Neurochem. 18:1387–1393.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.
Ernster, L., Zetterström, R., andLindberg, O. 1950. Phosphorus determination by the phosphomolybdate reagent. Acta Chem. Scand. 4:942–950.
Floridi, A., Fini, C., Palmerini, C. A., Mozzi, R., andPorcellati, G. 1979. High performance liquid chromatographic analysis of S-Adenosylmethionine and S-Adenosylhomocysteine in rat liver. J. Liquid Chromat. 2:1003–1015.
Kobayashi, M., Lust, W. D., andPassonneau, J. V. 1977. Concentration of energy metabolites and cyclic nucleotides during and after bilateral ischemia in the gerbil cerebral cortex. J. Neurochem. 29:53–59.
Marion, J., andWolfe, L. S. 1979. Origin of the arachidonic acid released post-mortem in rat forebrain. Biochim. Biophys. Acta 574:25–32.
Goracci, G., Francescangeli, E., Horrocks, L. A., andPorcellati, G. 1981. The reverse reaction of choline phosphotransferase in rat brain microsomes: a new pathway for degradation of phosphatidylcholine. Biochim. Biophys. Acta 664:373–379.
Mozzi, R., andPorcellati, G. 1982. Unpublished observations.
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This work was carried out by a research grant from the Consiglio Nazionale delle Ricerche (Rome, Italy) through the contribute CT82.02138.04/115.
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Trovarelli, G., De Medio, G.E., Porcellati, S. et al. The effect of S-adenosyl-l-methionine on ischemia-induced disturbances of brain phospholipid in the gerbil. Neurochem Res 8, 1597–1609 (1983). https://doi.org/10.1007/BF00964161
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DOI: https://doi.org/10.1007/BF00964161