Abstract
The distribution pattern and subcellular localisation of neuropeptide F (NPF) immunoreactivity (IR) in the tetrathyridium stage ofMesocestoides corti were investigated by whole-mount immunocytochemistry in conjunction with confocal scanning laser microscopy (CSLM) and by immunoelectron microscopy using immunogold labeling. Using an antiserum directed to the C-terminal decapeptide amide (residues 30–39) of synthetic NPF (Moniezia expansa), CSLM revealed NPF-IR throughout the central and peripheral nervous systems of parental and dividing tetrathyridia. Ultrastructurally, gold labeling of NPF-IR was confined to the contents of the smaller of the two sizes of electron-dense neuronal vesicle identified.
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Hrckova, G., Halton, D.W., Maule, A.G. et al. Neuropeptide F-immunoreactivity in the tetrathyridium ofMesocestoides corti (Cestoda: Cyclophyllidea). Parasitol Res 79, 690–695 (1993). https://doi.org/10.1007/BF00932512
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DOI: https://doi.org/10.1007/BF00932512