Summary
An immunohistochemical assay based on monoclonal antiestrophilin antibodies has been used to localize estrogen receptor (ER) in frozen sections of normal human endometrial, myometrial and cervical tissues from menstruating, hormonally treated, pregnant and postmenopausal women. Specific staining was confined to the cellular nuclei. In proliferative phase endometrium, postmenopausal emdometrium, and endometrium from patients treated with hormone ERs were easily detected in most glandular and stromal cells. After ovulation and in early pregnancy a quick and distinct decrease of ER expression was noted. This was especially the case with the more superficial layers of endometrium (endometrium functionals), the majority of whose cells had either weak localization of ER or none at all. In the endometrium basalis, however, the reduction of ER localization turned out to be more moderate. More then half of the epithelial and stromal cells displayed nuclear staining, partly strong. The myometrium of the corpus uteri showed a similar ER localization and dependence on hormonal stage when compared with the endometrium functionalis. The endocervical mucosa displayed a high degree of ER expression in the proliferative phase in postmenopausal women and in women who had been treated with hormones. Unlike the endometrium and myometrium, the endocervical glands underwent minimal changes in nuclear ER content during the menstrual cycle. Although the endocervical stroma showed cyclic alterations in ER levels, their reduction after ovulation was less marked than in the corresponding endometria. In cervical squamous epithelium ER localization was predominantly confined to the basal layers. In the course of cellular maturation, specific nuclear staining vanished. In the proliferative phase, after the menopause and in early pregnancy, the basal, parabasal and intermediate cells were specifically stained. In the postovulatory phase. However, nuclear staining was confined to the basal and parabasal cells. Hormonally treated squamous epithelia almost completely lacked nuclear ER localization.
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References
Bayard F, Damilano S, Robel P, Baulieu EE (1978). Cytoplasmic and nuclear estradiol and progesterone receptors in human endometrium. J Clin Endocrinol Metab 46:635–648
Beck Th, Pollow K, Heubner A, (1986) Monoklonale Antikörper zum immunhistochemischen Nachweis des Östrogenrezeptorstatus am Gewebeschnitt primärer Mammakarzinome. Geburtshilfe Frauenheilkd 46:490–494
Bergquist A, Ekman R, Ljungberg C (1985) Binding of estrogen and progesterone to human endometrium in the different phases of the menstrual cycle. A histochemical study. Am J Clin Pathol 83:444–449
Bur ME, Greene GL, Press MF (1987) Estrogen receptor localization in formalin-fixed, paraffin-embedded endometrium and endometriotic tissues. Int J Gynecol Pathol 6:140–151
Eiletz J, Genz T, Pollow K, Schmidt-Gollwitzer M (1980) Sex steroid levels in serum, myometrium, and fibromyomata in correlation with cytoplasmic receptors and 17 β-HSD activity in different age-groups and phases of the menstrual cycle. Arch Gynecol 229:13–28
Evans LH, Martin JD, Hähnel R (1974) Estrogen receptor concentration in normal and pathological human uterine tissues. J Clin Endocrinol Metab 38:23–32
Fagg B, Martin L (1979) Oestrogen content of the uterine tissues of mice and their relationship to epithelial cell: proliferation after subcutaneous and intraluminal administration of hormones. J Endocrinol 83:295–302
Fleming H, Namit C, Gurpide E (1980) Estrogen receptors in epithelial and stromal cells of human endometrium in culture. J Steroid Biochem 12:169
Gorski J, Toft D, Shyamala G, Smith D, Notides A (1968) Hormone receptors: studies on the interaction of estrogen with the uterus. Recent Prog Horm Res 24:45–80
Gould SF, Shannon JM, Cunha GR (1983) The autoradiographic demonstration of estrogen binding in normal human cervix and vagina during the menstrual cycle, pregnancy and the menopause. Am J Anat 168:229–238
Greene GL, Nolan C, Engler JP, Jensen EV (1980) Monoclonal antibodies to human estrogen receptor. Proc Natl Acad Sci USA 77:5115–5119
Greene GL, Jensen EV (1982) Monoclonal antibodies as probes for estrogen receptor detection and characterization. J Steroid Biochem 16:353–359
Greene GL, Sobel NB, King WJ, Jensen EV (1984) Immunochemical studies of estrogen receptors. J Steroid Biochem 20:51–56
Holt JA, Schumacher GFB, Jacobson HI, Swartz DP (1979) Estrogen and progestin binding and changes in secretions by human cervical tissue during the ovarian cycle Pertil Steril 32:170–176
Jensen EV, Suzuki T, Kawashuma T, Stumpf WE, Jungblut PW, De Sombre ER (1968) A two-step mechanism for the interaction of estradiol with rat uterus. Proc Natl Acad Sci USA 59:632–635
Jonat W, Stegner HE, Maass H (1985) Immunohistochemische Bestimmung von Östrogenrezeptoren im Mammakarzinomgewebe mittels monoclonaler Antikörper—erste klinische Ergebnisse. Geburtshilfe Frauenheilkd 45:473–476
King RJB, Townsend PT, Siddle N, Whitehead MI, Taylor RW (1982) Regulation of estrogen and progesterone receptor levels in epithelium and stroma from pre-and postmenopausal endometria. J Steroid Biochem 16:21–29
King WJ, Greene GL (1984) Monoclonal antibodies localize oestrogen receptor in the nuclei of target cells. Nature 307:745–747
Lantta M, Kärkkäinen J, Lehtovirta P (1983) Progesterone and estradiol receptors in the cytosol of the human uterine artery. Am J Obstet Gynecol 147:627–633
Levy C, Robel P, Gautray JP, De Brux J, Verma U, Descomps B, Baulieu EE (1980) Estradiol and progesterone receptors in human endometrium: normal and abnormal menstrual cycles and early pregnancy. Am J Obstet Gynecol 136:646–651
Martin PM, Rolland PH, Gammerre M, Serment H, Toga M (1979) Estradiol and progesterone receptors in normal and neoplastic endometrium: correlations between receptors. Histopathological examinations and clinical responses under progestin therapy. Int J Cancer 23:321
McClellan MC, West NB, Tacha DE, Greene GL, Brenner RM (1984) Immunocytochemical localization of estrogen receptors in the macaque reproductive tract with monoclonal antiestrophilins. Endocrinology 114:2002–2014
Natrajan PK, Muldoon TG, Greenblatt RB, Mahesh VB (1981) Estradiol and progesterone receptor in estrogen-primed endometrium. Am J Obster Gynecol 140:387–392
Noyes RW, Hertig AT, Rock J (1950) Dating the endometrial biopsy. Fertil Steril 1:3–10
Ochiai K (1980) Cyclic variation and distribution in the concentration of cytosol estrogen and progesterone receptors in the normal human ulterus and myoma. Acta Obstet Gynaecol Jpn 32:945–952
Padykula GA, Coles LG, Longscope C, King NW jr, McCraken JA (1983) Identification of epithelial progenitor cells in the cyclic primate endometrium. Biol Reprod [Suppl] 28:129
Piert M (1984) Östrogen- und Progesteronreceptoren in der Cervix uteri. Inaugural Dissertation Universität Köln
Press MF, Greene GL (1984) Methods in laboratory investigation. An immunocytochemical method for demonstrating estrogen receptor in human uterus using monoclonal antibodies to human estrophilin. Lab Invest 50:480–486
Press MF, Nousek-Goebl N, King WJ, Herbst AL, Greene GL (1984) Immunohistochemical assessment of estrogen receptor distribution in the human endometrium throughout the menstrual cycle. Lab Invest 51:495–503
Punnonen R, Pettersson K, Vanharanta R (1984) Androgen estrogen and progestin cytosol receptor concentrations in the human endometrium. Effects of intrauterine device. Gynecol Obstet Invest 17:73–77
Sanborn BM, Kuo HS, Held B (1978) Estrogen and progestogen binding site concentrations in human endometrium and cervix throughout the menstrual cycle and in tissue from women taking oral contraceptives. J Steroid Biochem 9:951–955
Sheridan PJ, Buchanan JM, Anselmo VC, Martin PM (1979) Equilibrium: the intracellular distribution of steroid receptors. Nature 282:579–582
Sternberger L, Hardy P, Cuculis J, Meyer H (1970) The unlabeled antibody enzyme method of immunhistochemistry. J Histochem 48:315–333
Yajima A, Wakisaka T, Wada Y, Suzuki M (1985) Tissue and intracellular distribution of estradiol receptors in the uterine endometrium. Gynecol Obstet Invest 19:97–102
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Dedicated to Professor V. Becker, Erlangen, on the occasion of his 65th anniversary
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Scharl, A., Vierbuchen, M., Graupner, J. et al. Immunohistochemical study of distribution of estrogen receptors in corpus and cervix uteri. Arch Gynecol Obstet 241, 221–233 (1988). https://doi.org/10.1007/BF00931353
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DOI: https://doi.org/10.1007/BF00931353