Abstract
Various lipids were extracted from tachyzoites and from purfied pellicles ofToxoplasma gondii. Extracts from both sources were found to have a low cholesterol/phospholipid ratio. The major phospholipid in these fractions was phosphatidylcholine associated with a low amount of sphingomyelin. Oleic acid represented one-third of whole-cell fatty acids and 44% of pellicular fatty acid content. The lipid composition of the pellicle ofT. gondii is consistent with the previously reported high fluidity of this membrane.
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References
Bottcher CJF, Van Gent CM, Pries C (1961) A rapid and sensitive sub-microphorus determination. Anal Clin Acta 24:203–204
Chaudhuri G, Banerjee AB (1987) Lipids and fatty acids ofLeishmania donovani donovani promastigotes. Indian J Med Res 85:140–148
Dawidowicz EA (1987) Dynamics of membrane lipid metabolism and turnover. Annu Rev Biochem 56:43–61
Folch J, Lees M, Sloanstanley GH (1961) In: Glick D (ed) Methods of biochemical analysis, vol. 2. Interscience Publishers, New York, pp 106–116
Foussard F, Gallois Y, Tronchin G, Robert R, Mauras G (1990) Isolation of the pellicle ofToxoplasma gondii (Protozoa, Coccidia). Characterization by electron microscopy and protein composition. Parasitol Res (in press)
Gallois Y, Foussard F, Girault A, Hodbert J, Tricaud A, Motta C (1988) Membrane fluidity ofToxoplasma gonii: a fluorescence polarization study. Biol Cell 62:11–15
Liebermann C (1885) Velder das oxychimoterpey. Ber 18:1803–1805
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Morrision WR, Smith LM (1964) Preparation of fatty acid methyl esters and dimethyl acetyls from lipids with boron fluoridemethanol. J Lipid Res 5:600–608
Philippe M, Schrevel J (1982) The three cortical membranes of the gregarines (parasitic protozoa). Characterization of the membrane proteins. Biochem J 201:455–464
Rouser G, Kritchevsky G, Yamamoto A (1967) Lipid chromatographic analysis. Dekker, New York
Shinitsky M, Inbar M (1976) Microviscosity parameters and protein mobility in biological membranes. Biochim Biophys Acta 533:133–149
Spector AA, Yoreck MA (1985) Membrane lipid composition and cellular function. J Lipid Res 26:1015–1035
Touchstone JC, Levin SS, Dolbins MF, Carfer PJ (1981) Differentiation of saturated and monounsaturated phospholipids on thin layer chromatograms. High Resolution Chromotogr Chromatogr Commun 4:423–424
Yechiel E, Barenholz Y (1985) Relationships between membrane lipid composition and biological properties of rat myocytes. J Biol Chem 260:9123–9131
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Foussard, F., Gallois, Y., Girault, A. et al. Lipids and fatty acids of tachyzoites and purified pellicles ofToxoplasma gondii . Parasitol Res 77, 475–477 (1991). https://doi.org/10.1007/BF00928412
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DOI: https://doi.org/10.1007/BF00928412