Abstract
Collagens present in the connective tissues of the extracellular matrix of fibrosarcoma were isolated and characterized. The fibrosarcoma was induced in rats by the administration of 3-methylcholanthrene. The results obtained were compared with normal muscle. An excess amount of type V collagen was found to be produced by the fibrosarcoma tissue compared to the normal muscle. Type V collagen from fibrosarcoma was characterized on the basis of solubility behavior in sodium chloride solutions, electrophoretic mobility on SDS-polyacrylamide gels, elution pattern of phosphocellulose chromatography and amino acid composition.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Krieg T, Hein A, Hatamochi A, Aumailley M: Molecular and clinical aspects of connective tissue. 18: 105–123, 1988
Liotta LA: Tumor Extracellular matrix. Lab Invest 47: 112–113, 1982
Miller EJ: (1984) Chemistry of the collagens and their distribution. In: KA Piez, AH Reddi (eds) Extracellular Matrix Biochemistry. Elsevier Science Publishing Co., New York
Prockop DJ: Mutations in collagen genes as a cause of connective tissue diseases. New Engl J Med 326: 540–546, 1992
Stenn KS, Madri JA, Roll FJ: Migrating epidermis produces AB2 collagen and requires continuous collagen synthesis for moment. Nature 277: 229–232, 1979
Squir CA, Kammayr GA: The role of connective tissue in the maintenance of epithelial differentiation in the adult. Cell Tissue Res 230: 615–630, 1983
Hayakawa T, Hashimoto Y, Myokei T, Aoyama H, Izawa Y: Changes in type of collagen during the development of human post-burn hypertrophic scars. Clin Chim Acta 93: 119–125, 1979
Murata K, Motayama T, Kotak C: Collagen types in various layers of the human aorta and their changes with the atherosclerotic process. Atherosclerosis 60: 251–262, 1986
Ehrlich HP, White BS: The identification of αA and αB collagen chains in hypertrophic scar. Exp Mol Pathol 34: 1–8, 1981
Miller EJ, Rhodes RK: Preparation and characterization of different types of collagens. Methods in Enzymology 82: 33–45, 1982
Sage H, Bornstein P: Characterization of a normal collagen chain in human placenta and its relation to AB collagen. Biochemistry 18: 3815–3822, 1979
Hay ED: Extracellular matrix. J Cell Biol 91: 205s-223s, 1981
Liotta LA, Rao CN, Barsky SH: Tumor invasion and the extracellular matrix. Lab Invest 49: 636–649, 1982
Liotta LA: Tumor invasion and metastases. Role of the extracellular matrix. Rhodes memorial award lecture. Cancer Res 46: 1–7, 1986
Jones PA, DeClerk YA: Extracellular matrix destruction by invasive tumor cells. Cancer Metastasis Rev 1: 289–317, 1982
Liotta LA, Thorgeirsson UP, Garbasia SH: Role of collagenases in tumor cell invasion. Cancer Metastasis Rev 1: 277–288, 1982
Krieg T, Timpl R, Kurkinen M, Veheri A: Type III procollagen is the major collagenous component produced by a continuous rhabdomyosarcoma cell line. FEBS Lett 104: 405–409, 1979
Stern R, Wilczek J, Thrope WP, Rosenberg SA, Cannon G: Procollagen as markers for the cell of origin of human bone tumors. Cancer Res 40: 325–328, 1980
Alitalo K, Keski-Oja J, Veheri A: Extracellular Matrix proteins characterize human tumor cell lines. Int J Cancer 27: 755–761, 1981
Barksy SH, Rao CN, Groteindorst GR, Liotta LA: Increased content of type V collagen in desmoplsia of human beast carcinoma. Am J Pathol 108: 276–283, 1982
Iozzo RV, Bolender RP, Wight TN: Proteoglycan changes in the intercellular matrix of human colon carcinoma. Lab Invest 47: 124–138, 1982
Bano M, Zwiebel JA, Salomon DS, Kidwell WR: Detection and partial characterization of collagen synthesis stimulating activities in rat mammary adenosarcomas. J Biol Chem 258: 2729–2735, 1983
Ooshima A: Collagen aB chains: Increased proportion in human athresclerosis. Science 213: 666–668, 1981
Narayanan AS, Clagott LA, Page RC: Effect of inflammation on the distribution of collagen types I, III, IV and V and type I trimer and fibronectin in human gingivae. J Dent Res 64: 1111–1116, 1985
Peltonen J, Kahari L, Uitto J, Jimenez SA: Increased expression of type VI collagen genes in systemic sclerosis. Arthritis Rheum 33: 1829–1835, 1990
Paulus W, Sage EH, Liszka U, Iruela-Arispe, Jellinger K: Increased levels of type VIII collagen in human brain tumours compared to normal brain tissue and non-neoplastic ceribral disorders. Br J Cancer 63: 367–371, 1991
Asokan R, Chandrakasan G, Puvanakrishnan R, Dhar SC: Separation and evaluation of changing pattern of glycosaminoglycans in 3-methylcholanthrene induced fibrosarcoma. Neoplasma 36: 273–279, 1989
Neuman RE, Logan MA: The determination of collagen and elastin in tissues. J Biol Chem 186: 549–556, 1950
Laemmli UK: Cleavage and structural proteins during assembly of the head of bacteriophage T4 Nature 277: 680–685, 1970
Black C, Douglas DM, Tanzer ML: Separation of cynogen bromide peptides of collagen by means of high performance liquid chromatography. J Chromatography 190: 393–400, 1980
Bornstein P, Sage H: Structurally distinct collagen types. Ann Rev Biochem 49: 957–1003, 1980
Heinrikson RL, Meredith SC: Amino acid analysis by reverse phase high performance liquid chromatography: Precolunn derivitization with phenylisothiocyanate. Anal Biochem 136: 65–74, 1984
Narayanan AS, Page RC, Meyers DF: Characterization of collagen of diseased human gingiva. Biochemistry 19: 5037–5042, 1980
Rhodes RK, Miller EJ: Physicochemical characterization and molecular organization of the collagen A and B chains. Biochemistry 17: 3442–3448, 1978
Uitto J, Perejda J. Connective tissue disease. Molecular pathology of the extracellular matrix. Part III: 249–542, 1987
Narayanan AS, Page RC: Synthesis of type V collagen by fibroblasts derived from normal, inflamed and hyperplastic human connective tissues. Collagen Rel Res 5: 297–304, 1985
Yamamoto M, Sumiyoshi H, Nakagami K, Tahara E: Distribution of collagen types I, III and V in fibrotic and neoplastic human liver. Acta Pathol (Jap) 34: 77–86, 1984
Marian B, Danner MW: Skin tumor promotion is associated with increased type V collagen content in dermis. Carcinogenesis 8: 151–154, 1987
Leushner JR, Most MD: Glycoproteins on the surface of smooth muscle cells involved in their interaction with type V collagen. Can J Biochem Cell Biol 63: 1176–1182, 1985
Mumby SM, Raugi GJ, Bornstein P: Light microscopic immunolocalization of thrombospondin in human tissues. J Cell Biol 92: 343–349, 1982
Kerenyi T, Voss B, Rauterberg J, Fromme HG, Jellinek H, Hauss WH: Connective tissue proteins on the injured endothelium of the rat aorta. Exp Mol Pathol 43: 151–161, 1985
Mayne R, Vail MS, Miller EJ: Characterization of the collagen chains synthesized by cultured smooth muscle cells derived from rhesus monkey thoracic aorta. Biochemistry 17: 446–452, 1978
Madri JA, Furthmayr H: Isolation and tissue localization of type AB2 collagen from lung parenchyma. Am J Pathol 94: 323–330, 1979
Martinez-Hernanez A, Gay S, Miller EJ: Ultrastructural localization of type V collagen in rat kidney. J Cell Biol 92: 343–349, 1982
Grotendorst GR, Seppa HE, Kleinman HK, Martin GR: Attachment of smooth muscle cells to collagen and their migration toward platelet derived growth factor. Proc Natl Acad Sci USA 78: 3669–3672, 1981
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Asokan, R., Reddy, G.K. & Dhar, S.C. Neoplastic association of enhanced type V collagen production in rat fibrosarcoma. Mol Cell Biochem 120, 25–32 (1993). https://doi.org/10.1007/BF00925981
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00925981