Abstract
The effects of age and hypertension on the antioxidant defence systems and the lipid peroxidation in rat isolated hepatocytes were studied. Four different age groups (1,3,6 and 12 months) were considered in spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats. Age-associated changes were observed on vitamin E status, glutathione (GSH) level, MDA formation and glutathione peroxidase (GSH-Px) activity in both strains. Maximal levels or activities of these parameters were found at 3 and 6 months, except for MDA which was low at 3 months. Then, a fall was observed at 12-month-old compared to 6-month values. In addition, GSH-Px activity was significantly lower in SHR than in WKY rats, except at the age of one month. The decrease of this enzyme activity could induce an increased cellular generation of radical species and lipid peroxidation, which might be link to hypertension.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Harman D: Aging: a theory based on free radical and radiation chemistry. J Gerontol 11: 298–300, 1956
Harman D: Free radical theory of aging: origin of life, evolution and aging. Age 3: 100–102, 1980
Player TJ, Mills DJ, Horton AA: Age-dependent changes in rat liver microsomal and mitochondrial NADPH-dependent lipid peroxidation. Biochem Biophys Res Commun 78: 1397–1402, 1977
Uysal M, Seckin S, Kocak-Toker N, Oz H: Increased hepatic lipid peroxidation in aged mice. Mech Ageing Dev 48: 85–89, 1989
Kanazawa K, Ashida H: Target enzymes on hepatic dysfunction caused by dietary products of lipid peroxidation. Arch Biochem Biophys 288: 71–78, 1991
Slater TF, Cheeseman KH, Davies MJ, Proudfoot K, Xin W: Free radical mechanisms in relation to tissue injury. Proc Nutr Soc 46: 1–12, 1987
Dargel R: Lipid peroxidation — a common pathogenetic mechanism? Exp Toxic Pathol 44: 169–181, 1992
Kannel WB, Tavia G: Evaluation of cardiovascular risk in the elderly: The Framingham study. Bull NY Acad Med 54: 573–591, 1978
Reed G, Anderson RJ: Epidemiology and risk of hypertension in the elderly. Clin Ther 5: 51–57, 1982
Pourrias B, Bailly Y: Mesure de la pression sanguine artérielle par voie non sanglante chez l'animal de laboratoire non anesthésié. Bull ATAL 512: 15–28, 1975
Seglen PO: Preparation of rat liver cells. In: Enzymatic Requirements for Tissues Dispersion. Exp Cell Res 82: 391–398, 1973
Skrede S, Narce M, Bergseth S, Bremer J: The effects of alkylthioacetic acids (3-thia fatty acids) on fatty acid metabolism in isolated hepatocytes. Biochim Biophys Acta 1005: 296–302, 1989
Paglia DE, Valentine WN: Studies on the quantitative and qualitative characterization of crythrocyte glutathione peroxidase. J Lab Clin Med 70: 158–169, 1967
Chaudière J, Gérard D: Dosage de l'activité glutathion peroxydase. In: L Douste-Blazy, F. Mendy (eds) Biologie des Lipides chez l'Homme. Editions Médicales Internationales, Paris, 1988, pp 275–279
neuschwander-Tetri BA, Roll FJ: Glutathione measurement by high-performance liquid chromatography separation and flurometric detection of the glutathione-orthophthalaldehyde adduct. Anal Biochem 179: 236–241, 1989
Calzada C, Véricel E, Lagarde M: Decrease in platelet reduced glutathione increases lipoxygenase activity and decreases vitamin E. Lipids 26: 696–699, 1991
Véricel E, Croset M, Sedivy P, Courpron Ph, Dechavanne M, Lagarde M: Platelets and aging. I-aggregation, arachidonate metabolism and antioxidant status. Thromb Res 49: 331–342, 1988
Therasse J, Lemonnier F: Determination of plasma lipoperoxides by high-performance liquid chromatography. J Chromatogr 413: 237–241, 1987
Harman D: Free radical theory of aging: the ‘free radical’ diseases. Age 7: 111–131, 1984
Rathbun WB, Bovis MG, Holleschau AM: Glutathione peroxidase, glutathione reductase and glutathione-S-transferase activities in the rhesus monkey lens as a function of age. Curr Eye Res 5: 195–199, 1986
Cand F, Verdetti J: Superoxide dismutase, glutathione peroxidase, catalase, and lipid peroxidation in the major organs of the aging rats. Free Radic Biol Med 7: 59–63, 1989
Rikans LE, Moore DR, Snowden CD: Sex-dependent differences in the effects of aging on antioxidant defense mechanisms of rat liver. Biochim Biophys Acta 1074: 195–200, 1991
Ji LL, Dillon D, Wu F: Alteration of antioxidant enzymes with aging in rat skeletal muscle and liver. Am J Physiol 258: R918-R923, 1990
Ito H, Torii M, Suzuki T: A comparative study on defense systems for lipid peroxidation by free radicals in spontaneously hypertensive and normotensive rat myocardium. Comp Biochem Physiol 1: 37–40, 1992
Farooqui MYH, Day WW, Zamorano DM: Glutathione and lipid peroxidation in the aging rat. Comp Biochem Physiol 88: 177–180, 1987
Hazelton GA, Lang CA: Glutathione content of tissues in the aging mouse. Biochem J 188: 25–30, 1980
Uhlig S, Wendel A: The physiological consequences of glutathione variations. Life Sci 51: 1083–1094, 1992
Chow CK: Vitamin E and oxidative stress. Free Radic Biol Med 11: 215–232, 1991
Vatassery GT, Johnson GJ, Krezowski AM: Changes in vitamin E concentrations in human plasma and platelets with age. J Amer Coll Nutr 4: 369–375, 1983
Hoekstra WG: Biochemical and function of selenium and its relation to vitamin E. Fed Proc 34: 2083–2089, 1975
Barja de Quiroga G, Pérez-Campo R, Lopez-Torres M: Anti-oxidant defences and peroxidation in liver and brain of aged rats. Biochem J 272: 247–250, 1990
Avissar N, Whitin JC, Allen PZ, Wagner DD, Liegey P, Cohen HJ: Plasma selenium-dependent glutathione peroxidase. J Biol Chem 264: 15850–15855, 1989
Gryglewski RJ, Palmer RMJ, Moncada S: Superoxide anion is involved in the breakdown of endothelium-derived vascular relaxing factor. Nature 320: 454–456, 1986
Moneada S, Palmer RMJ, Higgs EA: Platelet-vessel wall interactions: eicosanoids and EDRF. In: A Leaf, PC Weber (eds) Atheroselerosis Reviews. Raven Press Ltd., New York, 1990; 21: 71–77
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Véricel, E., Narce, M., Ulmann, L. et al. Age-related changes in antioxidant defence mechanisms and peroxidation in isolated hepatocytes from spontaneously hypertensive and normotensive rats. Mol Cell Biochem 132, 25–29 (1994). https://doi.org/10.1007/BF00925671
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00925671