Abstract
The expression of secretory component (SC) by human glandular endometrial cells cultured invitro was significantly increased by estradiol in the medium. Interferon-gamma and interleukin-4 stimulated the expression of SC only in the presence of estrogen. Tumor necrosis factor-alpha plus estrogen also caused a significant increase in the number of cells expressing SC. HLA class II antigen DR was detected on few glandular epithelial cells of human endometrium cultured in control medium, whereas interferon-gamma and interleukin-4, but not tumor necrosis factor-alpha, caused significant increases in the expression of DR. Estrogen in the culture medium did not significantly affect DR expression. The human endometrial adenocarcinoma cell lines, HEC and RL-95, expressed SC in approximately 50 and 20% of the cells. Also, approximately 20% of the RL-95 cells stained for DR antigen. Interferon-gamma did not influence the degree of expression of either surface marker of the two cell lines. Cells of both lines bound polymeric IgA and IgM but showed little to no binding of monomeric IgA, IgG, or an IgM previously shown not to bind SC.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Mestecky J, Kutteh WH, Ladjeva I, Peterman J: The common mucosal immune system in the human genital tract and mammary gland.In Reproductive Immunology 1989, L Mettler, WD Billington (eds). Amsterdam, Elsevier Science, 1990, pp 251–258
Kutteh WH, Edwards RP, Menge AC, Mestecky J: IgA Immunity in female reproductive tract secretions.In Proceedings of the Symposium on Local Immunity in Reproductive Tract Tissues, GP Talwar (ed). Cambridge, Cambridge University Press, 1992, pp 229–243
Mestecky J, McGhee JR: Immunoglobulin A (IgA): molecular and cellular interactions involved in IgA biosynthesis and immune response. Adv Immunol 40:154, 1987
Archibald DW, Witt DJ, Craven DE, Vogt MW, Hirsch MS, Essex M: Antibodies to human immunodeficiency virus in cervical secretions from women at risk for AIDS. J Infect Dis 156:240–241, 1987
Merriman H, Woods S, Winter C, Fahnlander A, Corey L: Secretory IgA antibody in cervicovaginal secretions from women with genital infection due to herpes simplex virus. J Infect Dis 149:505–510, 1984
O'Reilly RJ, Lee L, Welch BG: Secretory IgA antibody responses to Neisseria gonorrhoeae in the genital secretions of infected females. J Infect Dis 133:113–125, 1976
Richmond SJ, Milne D, Hilton AL, Caul EO: Antibodies to Chlamydia trachomatis in cervicovaginal secretions: Relation to serum antibodies and current chlamydial infection. Sex Trans Dis 7:11–15, 1980
Terho P, Meurman O: Chlamydial serum IgG, IgA and local IgA antibodies in patients with genital-tract infections measured by solid-phase radioimmunoassay. J Med Microbiol 14:77–87, 1981
Haas GG: Immunologic infertility. Obstet Gynecol Clin No Am 14:1069–1085, 1987
Brandtzaeg P: The role of J chain and secretory component in receptor-mediated glandular and hepatic transport of immunoglobulins in man. Scand J Immunol 139:3961–3969, 1985
Arends J, Groniowski M, de Koning Gans H, Bosman F: Immunohistochemical study of the distribution of secretory component and IgA in the normal and diseased uterine mucosa. Int J Gynecol Pathol 2:171–181, 1983
Kelly J, Fox H: The local immunological defence system of the human endometrium. J Reprod Immunol 1:39–45, 1979
Kutteh W, Hatch K, Blackwell R, Mestecky J: Secretory immune system of the female reproductive tract. I. Immunoglobulin and secretory component containing cells. Obstet Gynecol 71:56–60, 1988
Suzuki M, Ogawa M, Tamada T, Nagura H, Watanabe K: Immunohistochemical localization of secretory component and IgA in the human endometrium in relation to menstrual cycle. Acta Histochem Cytochem 17:223–229, 1984
Lee Y-S, Raju G: Expression of IgA and secretory component in the normal and in adenocarcinomas of Fallopian tube, endometrium and endocervix. Histopathology 13:67–78, 1988
Tabibzadeh S, Gerber M, Satyaswaroop P: Induction of HLA-DR antigen expression in human endometrial epithelial cells in vitro by recombinant γ-interferon. Am J Pathol 125:90–96, 1986
Kvale D, Lovhaug D, Soolid L, Brandtzaeg P: Tumor necrosis factor-α upregulates expression of secretory component, the epithelial receptor for polymeric Ig. J Immunol 140:3086–3089, 1988
Kvale D, Brandtzaeg P, Lovhaug D: Up-regulation of the expression of secretory component and HLA molecules in a human colonic cell line by tumor necrosis factor-α and gamma interferon. Scand J Immunol 28:351–357, 1988
Phillips J, Everson M, Moldoveanu Z, Lue C, Mestecky J: Synergistic effect of IL-4 and IFN-γ on the expression of polymeric Ig receptor (secretory component) and IgA binding by human epithelial cells. J Immunol 145:1740–1744, 1990
Sollid L, Kvale D, Brandtzaeg P, Markussen G, Thorsby E: Interferon enhances expression of secretory component, the epithelial receptor for polymeric immunoglobulins. J Immunol 138:4303–4306, 1987
Satyaswareep P, Bressler R, de la Pena M, Gurpide E: Isolation and culture of human endometrium in vitro. J Clin Endocrinol Metab 48:639–645, 1979
Osteen K, Hill G, Hargrove J, Gorstein F: Development of a method to isolate and culture highly purified populations of stromal and epithelial cells from human endometrial biopsy specimens. Fertil Steril 52:965–972, 1989
Mestecky J, Zikan J, Butler W, Kulhavy R: Studies on human secretory immunoglobulin A. III. J chain. Immunocytochemistry 9:883–991, 1972
Mestecky J, Kilian M: Immunoglobulin A (IgA). Methods Enzymol 116:37–75, 1985
Wira C, Bodwell J, Prabhala R: In vivo response of secretory component in the rat uterus to antigen, IFN-γ and estradiol. J Immunol 146:1893–1899, 1991
Valnes K, Brandtzaeg P, Elgjo K, Stave R: Specific and nonspecific humoral defense factors in the epithelium of normal and inflammed gastric mucosa: Immunohistochemical localization of immunoglobulins, secretory component, lysozyme and lactoferrin. Gastroenterology 86:402–412, 1984
Mazanec M, Nedrud J, Liang X, Lamm M: Transport of serum IgA into murine respiratory secretions and its implications for immunization strategies. J Immunol 142:4275–4279, 1989
Renegar K, Small P: Passive transfer of local immunity to influenza virus infection by IgA antibody. J Immunol 146:1972–1978, 1991
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Menge, A.C., Mestecky, J. Surface expression of secretory component and HLA class II DR antigen on glandular epithelial cells from human endometrium and two endometrial adenocarcinoma cell lines. J Clin Immunol 13, 259–264 (1993). https://doi.org/10.1007/BF00919384
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00919384