Abstract
Bacillus strain QC1-2, isolated from a chromium-polluted zone, was selected by its high ability to both tolerate and reduce hexavalent chromium [Cr(VI)] to less-toxic trivalent chromium [Cr(III)]. Cell suspensions of strain QC1-2 rapidly reduced Cr(VI), in both aerobic and anaerobic conditions, to Cr(III) which remained in the supernatant. Cr(VI) reduction was dependent on the addition of glucose but sulfate, an inhibitor of chromate transport, had no effect. Studies with permeabilized cells and cell extracts showed that the Cr(VI) reductase of strain QC1-2 is a soluble NADH-dependent enzyme.
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American Public Health Association (1971) Chromium, pp 115–159. In: Standard Methods for the Examination of Water and Wastewater. Amer. Public Health Assoc., New York
Basabe JR, Lee CA & Weiss RL (1979) Enzyme assays using permeabilized cells ofNeurospora. Anal. Biochem. 92: 356–360
Bopp LH & Ehrlich HL (1988) Chromate resistance and reduction inPseudomonas fluorescens LB300. Arch. Microbiol. 150: 426–431
Cervantes C (1991) Bacterial interactions with chromate. Antonie van Leeuwenhoek 59: 229–233
Cervantes C & Ohtake H (1988) Plasmid-determined chromate resistance inPseudomonas aeruginosa. FEMS Microbiol. Lett. 56: 173–176
Cervantes C & Silver S (1992) Bacterial chromate resistance and chromate reduction. Plasmid 27: 65–71
Das S & Chandra AL (1990) Chromate reduction inStreptomyces. Experientia 46: 731–733
Gvozdyak PL, Mogilavich NF, Rylskii AF & Grishchenko NI (1986) Reduction of hexavalent chromium by collection strains of bacteria. Mikrobiologiya 55: 962–965
Holt JG (Ed.) (1977) The shorter Bergey's Manual of Determinative Microbiology. 8th Ed. Williams & Wilkins, Baltimore
Horitsu H, Futo S, Miyazawa Y, Ogai S & Kawai K (1987) Enzymatic reduction of hexavalent chromium by hexavalent chromium tolerantPseudomonas ambigua G-1. Agric. Biol. Chem. 51: 2417–2420
Ishibashi Y, Cervantes C & Silver S (1990) Chromium reduction inPseudomonas putida. Appl. Environ. Microbiol. 56: 2268–2270
Lowry OH, Rosebrough NJ, Farr AL & Randall RJ (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275
Ohtake H, Cervantes C & Silver S (1987) Decreased chromate uptake inPseudomonas fluorescens carrying a chromate resistance plasmid. J. Bacteriol. 169: 3853–3856
Suzuki T, Miyata N, Horitsu H, Kawai K, Takamizawa K, Tai Y & Okazaki M (1992) NAD(P)H-dependent chromium (VI) reductase ofPseudomonas ambigua G-1: a Cr(V) intermediate is formed during the reduction of Cr(VI) to Cr(III). J. Bacteriol. 174: 5340–5345
Wang PC, Mori T, Komori K, Sasatsu M, Toda K & Ohtake H (1989) Isolation and characterization of anEnterobacter cloacae strain that reduces hexavalent chromium under anaerobic conditions. Appl. Environ. Microbiol. 55: 1665–1669
Wang PC, Mori T, Toda K & Ohtake H (1990) Membrane-associated chromate reductase activity fromEnterobacter cloacae. J. Bacteriol. 172: 1670–1672
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Campos, J., Martinez-Pacheco, M. & Cervantes, C. Hexavalent-chromium reduction by a chromate-resistantBacillus sp. strain. Antonie van Leeuwenhoek 68, 203–208 (1995). https://doi.org/10.1007/BF00871816
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DOI: https://doi.org/10.1007/BF00871816