Summary
The self-sterility ofHalocynthia roretzi from Mutsu Bay, Japan, was examined. This sterility is strict and not a single egg can be fertilized in self-sterile animals. Less than 2% of the animals were self-fertile (with 100% cross-fertility). All heterologous sperm can fertilize all eggs, although there are pairs of individuals in which the coelomocytes recognize each other as self. Eggs deprived of follicle cells cannot be fertilized by either autologous or heterologous spermatozoa. Detached autologous or heterologous follicle cells can reattach to the chorion in calcium-enriched sea water and the reconstituted eggs recover their ability to be fertilized. A “mosaic egg” can therefore be obtained, which consists of oocyte, test cells and chorion originating from one individual and follicle cells from another. The “mosaic egg” was used to determine the site of recognition of self and non-self. The results indicate that the recognition resides in the chorion and/or test cells, probably the chorion. The relationship between somatic alloreactivity, previously found in coelomocytes ofH. roretzi, and gamete reactivity is discussed.
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References
Fuke TM (1980) “Contact reaction” between xenogeneic or allogeneic coelomic cells of solitary ascidians. Biol Bull 158:304–305
Fuke TM, Numakunai T (1982) Allogeneic cellular reactions between intra-specific types of a solitary ascidian,Halocynthia roretzi. Dev Com Immunol 6:253–261
Hoshi H, Numakunai T, Sawada H (1981) Evidence for participation of sperm proteinases in fertilization of solitary ascidian,Halocynthia roretzi: effects of protease inhibitors. Dev Biol 86:117–121
Ishikawa M, Numakunai T, Kubo M (1978) Fertilization in ascidians: Interaction between spermatozoa and eggs. In: Abstract. 11th Annual Meeting of Japanese Society for Developmental Biology 74 [in Japanese]
Jaffery WR (1980) The follicular envelopes of ascidian eggs: a site of messenger RNA and protein synthesis during early embryogenesis. J Exp Zool 212:279–289
Lambert CC, Lambert G (1978) Tunicate eggs utilized ammonium ions for fertilization. Science 200:64–65
Miller RL (1975) Chemotaxis of spermatozoa ofCiona intestinalis. Nature [London] 254:244–245
Morgan TH (1923) Removal of the block to self fertilization in the ascidian Ciona. Proc Nat Acad Sci USA 9:170–171
Morgan TH (1942) Do spermatozoa penetrate the membrane of self-inseminated eggs of Ciona and Styela? Biol Bull 82:455–460
Numakunai T, Hoshino Z (1980) Periodic spawning of three types of the ascidian,Halocynthia roretzi (Drashe), under continuous light conditions. J Exp Zool 212:381–387
Numakunai T, Hoshino Z, Hori R (1981) Biology of the ascidian,Halocynthia roretzi (Drashe) in Mutsu Bay. III Distribution and external characteristics of three types. Ann Zool Jap 54:230–239
Oka H, Watanabe H (1967) On the colony specificity: with particular reference to fusibility of compound ascidians. Kagaku 37:307–313 [in Japanese]
Oka H (1970) Colony specificity in compound ascidians: The genetic control of fusibility. In: H. Yukawa (ed). Profiles of Japan and Japanese scientists. Kodansha, Tokyo, pp 196–206
Pinto MR, De Santis R, D'Allessio G, Rosati F (1981) Studies on fertilization in the ascidians: fucosyl sites on vitelline coat ofCiona intestinalis. Exp Cell Res 132:289–295
Rosati F, De Santis R (1978a) Studies on fertilization in ascidians. I. Self-sterility and specific recognition between gametes ofCiona intestinalis. Exp Cell Res 112:111–119
Rosati F, De Santis R (1978b) Studies on fertilization of the ascidian II. Lectin binding to the gametes ofCiona intestinalis. Exp Cell Res 116:419–427
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Fuke, M.T. Self and non-self recognition between gametes of the ascidian,Halocynthia roretzi . Wilhelm Roux' Archiv 192, 347–352 (1983). https://doi.org/10.1007/BF00848815
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DOI: https://doi.org/10.1007/BF00848815