Abstract
Electron microscopy (EM) in combination with image analysis is a powerful technique to study protein structure at low- and high resolution. Since electron micrographs of biological objects are very noisy, substantial improvement of image quality can be obtained by averaging individual projections. Crystallographic and noncrystallographic averaging methods are available and have been applied to study projections of the large protein complexes embedded in photosynthetic membranes from cyanobacteria and higher plants. Results of EM on monomeric and trimeric Photosystem I complexes, on monomeric and dimeric Photosystem II complexes, and on the monomeric cytochromeb6/f complex are discussed.
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Boekema, E.J., Boonstra, A.F., Dekker, J.P. et al. Electron microscopic structural analysis of Photosystem I, Photosystem II, and the cytochromeb6/f complex from green plants and cyanobacteria. J Bioenerg Biomembr 26, 17–29 (1994). https://doi.org/10.1007/BF00763217
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DOI: https://doi.org/10.1007/BF00763217