Abstract
Two general approaches have been used to define genetically the genes that encode components of the cellular protein export machinery. One of these strategies identifies mutations that confer a conditional-lethal, pleiotropic export defect (sec,secretion). The other identifies dominant suppressors of signal sequence mutations (prl,proteinlocalization). Subsequent characterization reveals that in at least three cases,prlA/secY,prlD/secA, andprlG/secE, both types of mutations are found within the same structural gene. This convergence is satisfying and provides compelling evidence for direct involvement of these gene products in the export process.
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Bieker, K.L., Phillips, G.J. & Silhavy, T.J. Thesec andprl genes ofEscherichia coli . J Bioenerg Biomembr 22, 291–310 (1990). https://doi.org/10.1007/BF00763169
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DOI: https://doi.org/10.1007/BF00763169