Zusammenfassung
An der Niere der Albinomaus wurde nach Vorfixierung mit Osmiumtetroxyd eine modifizierte Gomori-Reaktion auf Phosphatasen durchgeführt. Als Fällungsmittel der bei dem Test freigesetzten Orthophosphorsäure wurde ein Bleisalz verwendet. Damit konnte im elektronenmikroskopischen Bild am Enzymort ein feinkörniger Niederschlag mit hohem Kontrast erzielt werden. Als Sitz der beiph 7,6–7,7 wirksamen Phosphatasen konnten die Membran des Bürstensaums und die basalen Einstülpungen der Zellmembran der Tubulusepithelzellen erkannt werden. Enzymatisch aktiv wurden ferner die Zellmembranen der Kapillarendothelien des Tubulus und des Glomerulus sowie die Deckzellfortsätze (Microvilli) des Glomerulus befunden.
Das Problem der intrazellulären Lokalisation von Phosphatasen wird diskutiert.
Summary
A modified Gomori-reaction on phosphatases of the albino-mouse kidney was performed. The method of prefixation with osmiumtetroxyde was used. The orthophosphoric acid, liberated in the test and cristallized by lead salt, yielded a fine granulated sediment with a high contrast at the location of enzyme in the electronmicroscopic picture. The phosphatases, active at aph of 7,6–7,7, could be located in the membranes of the brushborder and in the basal invaginations of the membranes of the tubuli cells. Furthermore an enzymatic activity was observed in the membranes of the capillary endothelium cells in the tubulus as well as in the glomerulus and in the microvilli of the glomerulus.
Problems resulting from the test for the phosphatase were discussed.
Article PDF
Avoid common mistakes on your manuscript.
Literatur
Andersson-Cedergreen, E.: Ultrastructure of motor end plate and sarcoplasmic components of mouse skeletal muscle fiber. J. ultrastruct. Res. Suppl.1 (1959).
Barrnett, R. J.: Histochemical demonstration of dehydrogenase systems requiring pyridine nucleotide co-enzymes, with the electron microscop. Anat. Rec.127, 395 (1957).
Histochemistry and electron microscopy. 4. Internat. Kongr. Elektr.mikrosk., Berlin, 1958.
The demonstration with the electron microscope of the endproducts of histochemical reactions in relation to the fine structure of cells. Exp. Cell Res., Suppl.7, 65–89 (1959).
Barrnett, R. J., andG. E. Palade: Histochemical demonstration of the sites of activity of dehydrogenase systems with the electron microscope. J. biophys. biochem. Cytol.3, 577–588 (1957).
Application of histochemistry to electron microscopy. J. Histochem. Cytochem.6, 1–12 (1958).
Enzymatic activity in the M-band. J. biophys. biochem. Cytol.6, 163–169 (1959).
Essner, E., A. B. Novikoff andB. Masek: Adenosinetriphosphatase and 5-nucleotidase activities in the plasma membrane of liver cells as revealed by electron microscopy. J. biophys. biochem. Cytol.4, 711–716 (1958).
Gersh, J.: Selective and cytochemical staining of frozen-dried preparations for study with the electron microscope. 4. Internat. Kongr. Elektr.mikrosk., Berlin, 1958.
Gomori, G., andE. P. Benditt: Precipitation of calcium phosphate in the histochemical method for phosphatase. J. Histochem. Cytochem.1, 114–122 (1953).
Johansen, G., andK. Linderström-Lang: Precipitation of phosphate in the Gomori-test. Acta chem. scand.5, 965–967 (1951).
Liberation, diffusion and precipitation of phosphate in the Gomori-test. Acta med. scand.142, 601–613 (1952).
Liberation, diffusion and precipitation of phosphate in the Gomori-test. J. Histochem. Cytochem.1, 442–446 (1953).
Kaplan, S. E., andA. B. Novikoff: The localisation of adenosine triphosphatase activity in rat kidney. J. Histochem. Cytochem.7, 295 (1959).
Lison, L.: La recherche histochimique des phosphates. Etude critique. Bull. Histol. Techn. micr.25, 23–41 (1948).
Mölbert, E., F. Duspiva u.O. v. Deimling: Die Darstellung der Phosphatasen im elektronenmikroskopischen Bild. J. biophys. biochem. Cytol. (im Druck).
Novikoff, A. B.: The validity of histochemical phosphatase methods on the intracellular level. Science113, 320–325 (1951).
Pearse, A. G. E.: Histochemistry. London: Churchill 1954.
Rhodin, J.: Electron microscopy of the kidney. Amer. J. Med.24, 661–675 (1958).
Robertson, J. S.: Ordering in lamellar systems. 4. Internat. Kongr. Elektr.mikrosk., Berlin, 1958.
Ruska, H., D. H. Moore andJ. Weinstock: The base of the proximal convoluted tubule cells of rat kidney. J. biophys. biochem. Cytol.3, 249–254 (1957).
Sjöstrand, F. S., andJ. Rhodin: The ultrastructure of the proximal convoluted tubules of the mouse kidney as revealed by high resolution electron microscopy. Exp. Cell. Res.4, 426–456 (1953).
Spater, H. W., A. B. Novikoff andB. Musek: Adenosine triphosphatase activity in the cell membranes of kidney tubule cells. J. biophys. biochem. Cytol.4, 765 (1958).
Unakar, N. J., andS. M. Sirsat: Distribution of succinic dehydrogenase in the human spermatozoa as revealed in the electron microscope. 4. Internat. Kongr. Elektr.mikrosk., Berlin, 1958.
Watson, M. L.: Staining of tissue section for electron microscopy with heavy metals. J. biophys. biochem. Cytol.4, 475–478 (1958).
Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium. J. biophys. biochem. Cytol.4, 727–730 (1958).
Author information
Authors and Affiliations
Additional information
Mit 17 Textabbildungen
Mit Unterstützung des Bundes und der Deutschen Forschungsgemeinschaft. Über die Ergebnisse wurde auf der 9. Tagung der Deutschen Gesellschaft für Elektronenmikroskopie vom 18.–21. 10, 59 in Freiburg i. Br. berichtet.
Rights and permissions
About this article
Cite this article
Mölbert, E., Duspiva, F. & v. Deimling, O. Die histochemische Lokalisation der Phosphatase in der Tubulusepithelzelle der Mäuseniere im elektronenmikroskopischen Bild. Histochemie 2, 5–22 (1960). https://doi.org/10.1007/BF00736487
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00736487