Summary
The localization of S-protein/Vitronectin as deposits in arterial lesions and as a component of extracellular matrices was investigated by indirect immunofluorescence in ten atherosclerotic samples of carotid endarteriectomy compaired with ten arteriosclerotic temporal biopsies. Anti-C5b-9 neoantigens, anti-C3, anti-C3d, anti-H, anti-IgG and anti-IgM antibodies were applied on serial sections. In the atherosclerotic plaque, S-protein deposits were observed as irregular granules and spots in the fibrous cap and the internal part of the media at the vicinity of the plaque; they inconstantly colocalized with C5b-9 neoantigens. When present the SC5b-9 complexes were generally associated with cell remnants in the sclerotic matrix. In the temporal artery biopsies, S-protein was bound exclusively to the internal elastic lamnina in association with C3d, but was absent from the intimal fibrous thickening. S-protein was not detected as a diffuse component of the extra-cellular matrix of either musculo-elastic or muscular medias, but was clearly demonstrated in smaller arteries; this result suggests a differential distribution of S-protein along the aterial tree.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bhakdi S, Tranum-Jensen J (1982) Terminal membrane C5b-9 complex of human complement: a transition from an amphophilic to a hydrophilic state through binding of the S-protein from serum. J Cell Biol 94:755–759
Bhakdi S, Tranum-Jansen J (1983) Membrane damage by complement. Biochimica et Biophysica Acta 737:343–372
Dahlbäck K, Löfberg H, Dahlbäck B (1986) Localization of Vitronectin (S-protein of Complement) in normal human skin. Acta Derm Venereol (Stockh) 66:461–467
Falk RJ, Podack E, Dalmasso AP, Jennette JC (1987) Localization of S-protein and its relationship to the membrane attack complex in renal tissue. Am J Pathol 127:182–190
Gebb C, Hayman EG, Engvall E, Ruoslahti E (1986) Interaction of Vitronectin with collagen. J Biol Chem 261:16698–16703
Hansson GK, Holm J, Kral JG (1984) Accumulation of IgG and complement factor C3 in human arterial endothelium and atherosclerotic lesions. Acta Path Microbiol Immunol Scand, Sect A 92:429–435
Hayman EG, Piersbacher MD, Öhrgren Y, Ruootslahti E (1983) Serum spreding factor (vitronectin) is present at the cell surface and in tissues. Proc Natl Sci (USA) 80:4003–4007
Hinglais N, Kazatchkine MD, Bhakdi S, Appay MD, Mandet C, Grossete J, Bariety J (1986) Immunohistochemical study of the C5b-9 complex of complement in human kidneys. Kidney International 30:399–410
Niculescu F, Rus HG, Vlaicu R (1987) Immunohistochemical localization of C5b-9, S-protein, C3d and apolipoprotein B in human arterial tissues with atherosclerosis. Atherosclerosis 65:1–11
Rush HG, Nicukescu F, Constantinescu E, Cristea A, Vlaicu R (1986) Immunoelectron-microscopic localization of the terminal C5b-9 complement complex in human atherosclerotic fibrous plaque. Atherosclerosis 61:35–42
Simonton SC, Basara ML, Barnes DW, Furcht LT (1985) Distribution and immunolocalization of serum-spreading factor in human tissue. Lab Invest 52:63A
Tomasini BR, Mosher DF (1986) On the identity of Vitronectin and S-protein: immunological crossreactivity and functional studies. Blood 68:737–742
Vlaicu R, Niculescu F, Rus HG, Cristea A (1985) Immunohistochemical localization of the terminal C5b-9 complement complex in human aortic fibrous plaque. Atherosclerosis 57:163–177
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Guettier, C., Hinglais, N., Bruneval, P. et al. Immunohistochemical localization of S protein/vitronectin in human atherosclerotic versus arteriosclerotic arteries. Vichows Archiv A Pathol Anat 414, 309–313 (1989). https://doi.org/10.1007/BF00734084
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00734084