Abstract
The sialidase of swine influenza A viruses of N1 and N2 subtypes, isolated from 1930 to 1992, was studied for substrate specificity with ganglio-series, lacto-series type II and GM3 gangliosides containing Neu5Acα2-3Gal, Neu5Gcα2-3Gal and Neu5Acα2-6Gal linkages. All viral sialidases tested showed that the activity for hydrolysing substrates with Neu5Acα2-3Gal was higher than the activities with Neu5Gcα2-3Gal and Neu5Acα2-6Gal linkages. When GM1b, GM3 and sialylparagloboside were used as substrates, the earliest strain (A/Wisconsin/15/30 H1N1, isolated in 1930) showed the activity ratio of Neu5Acα2-6Gal to Neu5Acα2-3Gal to be 0.13:0.2, and the ratio Neu5Gcα2-3Gal/Neu5Acα2-3Gal to be 0.19:0.37, while those strains isolated from 1978 to 1992 exhibited ratios of 0.29:0.58 for Neu5Acα2-6Gal/Neu5Acα2-3Gal and 0.51:0.76 for Neu5Gcα2-3Gal/Neu5Acα2-3Gal. The above results indicate that the substrate specificities of sialidases from swine influenza A viruses towards sialyl linkages and the molecular species of sialic acid are related to the year of isolation, i.e. strains isolated after 1978 exhibited higher activity towards Neu5Acα2-6Gal and Neu5Gcα2-3Gal linkages when compared with strains isolated in an earlier year, 1930.
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Abbreviations
- Neu5Ac:
-
5-N-acetylneuraminic acid
- Neu5Gc:
-
5-N-glycolyneuraminic acid
- Gal:
-
d-galactose
- Glc:
-
d-glucose
- Cer:
-
Ceramide
- II3(Neu5Ac)Lac:
-
Neu5Acα2-3Galβ1-4Glc
- GM3(Neu5Acα2-3Gal):
-
Neu5Acα2-3Galβ1-4Glcβ1-Cer
- GM3(Neu5Gcα2-3Gal):
-
Neu5Gcα2-3Galβ1-4Glcβ1-Cer
- GM1b(Neu5Acα2-3Gal):
-
Neu5Acα2-3Galβ1-3GalNacβ1-4Galβ1-4Glcβ1-Cer
- GMlb(Neu5Gcα2-3Gal):
-
Neu5Gcα2-3Galβ1-3GalNAcβ1-4Galβ1-4Glcβ1-Cer
- IV3(Neu5Ac)nLc4Cer:
-
Neu5Acα2-3Galβ1-3GlcNAcβ1-4Galβ1-4Glcβ1-Cer
- IV3(Neu5Gc)nLc4Cer:
-
Neu5Gcα2-3Galβ1-3GlcNAcβ1-4Galβ1-4Glcβ1-Cer
- IV6(Neu5Ac)nLc4Cer:
-
Neu5Acα2-6Galβ1-3GlcNAcβ1-4Galβ1-4Glcβ1-Cer
- TDC:
-
taurodeoxycholate.
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Xu, G., Suzuki, T., Maejima, Y. et al. Sialidase of swine influenza A viruses: variation of the recognition specificities for sialyl linkages and for the molecular species of sialic acid with the year of isolation. Glycoconjugate J 12, 156–161 (1995). https://doi.org/10.1007/BF00731360
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DOI: https://doi.org/10.1007/BF00731360