Abstract
Electrophoretic banding pattens of total soluble cell proteins, DNA restriction fragments and chromosomal DNA were used to characterise ten strains ofSaccharomyces cerevisiae used for commercial production of wine. These fingerprinting procedures provided unique profiles for all the different yeast strains and can therefore be used to identify and control industrial strains. Furthermore, the protein profiles, restriction fragments banding patterns and electrophoretic karyotyping by contour clamped homogeneous electric field electrophoresis (CHEF), were valuable to differentiate hybrid and parental strains in yeast breeding programmes. Hybrid strains, with desirable oenological properties, were obtained by mass spore-cell mating between a heterothallic killer yeast and two homothallic sensitive strains and all were shown to have unique DNA fingerprints and electrophoretic karyotypes.
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Aigle M, Erbs D & Moll M (1983) Determination of brewing yeast ploidy by DNA measurement. J. Inst. Brew. 89: 72–74
Augustyn OPH & Kock JLF (1989) Differentiation of yeast species, and strains within a species, by cellular fatty acid analysis. 1. Application of an adopted technique to differentiate between strains ofSaccharomyces cerevisiae. J. Microbiol. Meth. 10: 9–23
Braus G, Furter R, Prantl F, Niederberger P & Hütter R (1985) Arrangement of genesTRP1 andTRP3 ofSaccharomyces cerevisiae strains. Arch. Microbiol. 142: 383–388
Carle GF & Oslon MV (1985) An electrophoretic karyotype for yeast. Proc. Natl. Acad. Sci. USA 82: 3756–3760
Casey GP, Xiao W & Rank GH (1988) Application of pulsed field chromosome electrophoresis in the study of chromosome XIII and the electrophoretic karyotype of industrial strains ofSaccharomyces yeasts. J. Inst. Brew. 94: 239–243
Casey GP & Pringle AT (1990) The relationship between yeast karyotype and fermentation performance. Eur. Brew. Conv. Proc. Cong. 23rd, Copenhagen (pp 1–9)
Decock JP & Iserentant D (1985) Ty hybridisation patterns of yeast and their use for strain identification. Eur. Brew. Conv. Proc. Cong. 20th, Helsinki (pp 195–202)
DeJonge P, Jonge FCM de, Meijers R, Steensma HY & Scheffers WA (1986) Orthogonal-field-altenation gel electrophoresis banding patterns of DNA from yeasts. Yeast 2: 193–204
Gupta NJ & Jones KW (1987) A DNA sequence which shows genomic variation ina, alpha andHO strains ofSaccharomyces cerevisiae. Curr. Genet. 12: 161–166
Hansen M, Röcken W & Emeis C-C (1990) Construction of yeast strains for the production of low-carbohydrate beer. J. Inst. Brew. 96: 125–129
Johnston JR, Contopoulou CR & Mortimer RK (1989) Karyotyping of yeast strains of several genera by field inversion gel electrophoresis. Yeast 4: 191–198
Johnston JR & Mortimer RK (1986) Electrophoretic karyotyping of laboratory and commercial strains ofSaccharomyces and other yeasts. Int. J. Syst. Bacteriol. 36: 569–572
Keiding AK (1985) Genetic and molecular characterization of a distiller's yeast. Carlsberg Res. Commun. 50: 95–125
Kunkee RE & Amerine MA (1970) Yeast in wine-making. In: Harrison JS & Rose AH (Eds). The Yeast, Vol III (pp 5–57) Academic Press, New York
Laaser G, Möller E, Janke K-D, Bahnweg G, Prillinger H & Prell HH (1989) Ribosomal DNA restriction fragment analysis as a taxonomic tool in separating physiologically similar basidiomycetous yeasts. System. Appl. Microbiol. 11: 170–175
Lee SY & Knudsen FB (1985) Differentiation of brewery yeast strains by restriction endonuclease analysis of their mitochondrial DNA. J. Inst. Brew. 91: 169–173
Martens FB, Berg R van den & Harteveld PA (1985) DNA fingerprinting of yeast strains. Eur. Brew. Conv. Proc. Cong. 20th, Helsinki (pp 211–217)
Meaden P (1990) DNA fingerprinting of brewers' yeast: current perspectives. J. Inst. Brew. 96: 195–200
Panchal CJ, Bast L, Dowhanick T & Stewart GG (1987) A rapid, simple and reliable method of differentiating brewing yeast strains based on DNA restriction patterns. J. Inst. Brew. 93: 325–327
Pedersen MB (1983a) DNA sequence polymorphisms in the genusSaccharomyces. I. Comparison of theHIS4 and ribosomal RNA genes in lager strains, ale strains and various species. Carlsberg Res. Commun. 48: 485–503
(1983b) Fingerprinting of DNA in the identification of yeast. Eur. Brew. Conv. Proc. Cong. 19th, London (pp 457–463)
(1985a) Brewing yeast identification by DNA fingerprinting. Eur. Brew. Conv. Proc. Cong. 20th, Helsinki (pp 202–210)
— (1985b) DNA sequence polymorphisms in the genusSaccharomyces. II. Analysis of the genusRDN1, HIS4, LEU2 andTy transposable elements in Carlsberg, Tuborg and 22 Bavarian brewing strains. Carlsberg Res. Commun. 50: 263–272
— (1986a) DNA sequence polymorphisms in the genusSaccharomyces. III. Restriction endonuclease fragment patterns of chromosomal regions in brewing and other yeast strains. Carlsberg Res. Commun. 51: 163–183
— (1986b) DNA sequence polymorphisms in the genusSaccharomyces. IV. Homologous chromosomes III ofSaccharomyces bayanus, S. carlsbergensis andS. uvarum. Carlsberg Res. Commun. 51: 185–202
Petering J, Langridge P & Henschke P (1988) Fingerprinting wine yeast. Aust. N.Z. Wine Ind. J. 3: 48–52
(1990) Identification of yeast strains by molecular biology techniques. International Association for Modern Winery Technology and Management e.V. Oenol. Symp. 9th, Cascais
Plummer DT (1971) An Introduction to Practical Biochemistry. McGraw-Hill Book Co. Ltd., London
Pretorius IS & Marmur J (1988) Localization of yeast glucoamylase genes by PFGE and OFAGE. Curr. Genet. 14: 9–13
Pretorius IS & Westhuizen TJ van der (1991) Impact of yeast genetics and recombinant DNA technology on the wine industry. S. Afr. J. Enol. Vitic. 12: 3–31
Sakai A, Shimizu Y & Hishinuma F (1990) Integration of heterologous genes into the chromosome ofSaccharomyces cerevisiae using a delta sequence of yeast retrotransposon Ty. Appl. Microbiol. Biotechnol. 33: 302–306
Salmon J-M, Pinon R & Gancedo C (1989) Isolation and characterization of mutants ofSaccharomyces cerevisiae able to sporulate in the presence of glucose. J. Gen. Microbiol. 135: 203–209
Schwartz DC & Cantor CR (1984) Separation of chromosomalsized DNAs pulsed field gradient gel electrophoresis. Cell 37: 67–75
Seehaus T, Rodicio R, Heinisch J, Aguilera A, Schmitt HD & Zimmerman FK (1985) Specific gene probes as tools in yeast taxonomy. Curr. Genet. 10: 103–110
Sherman F, Fink GR & Hicks JB (1986) Laboratory Course Manual for Methods in Yeast Genetics: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sor F & Fukuhara H (1989) Analysis of chromosomal DNA patterns of the genusKluyveromyces. Yeast 5: 1–10
Takata Y, Watari J, Nishikawa N & Kamada K (1990) Electrophoretic banding patterns of chromosomal DNA from yeasts. Am. Soc. Brew. Chem. 47: 109–113
Thornton RJ (1983) New yeast strains from old-the application of genetics to wine yeasts. Food Technol. Australia 35: 46–50
Tredoux HG, Kock JLF, Lategan PM & Muller HB (1987) A rapid identification technique to differentiate betweenSaccharomyces cerevisiae strains and other yeast species in the wine industry. Am. J. Enol. Vitic. 38: 161–164
Van der Westhuizen TJ & Pretorius IS (1989) Genetic characterization and breeding of wine yeasts. S. Afr. Soc. Enol. Vitic. Cong. 13th, Cape Town
(1990) Genetic characterization and breeding of wine yeasts. S. Afr. Soc. Microbiol. Cong. 6th, Stellenbosch (pp 15.15)
VanVuuren HJJ & Meer LJ van der (1987) Fingerprinting of yeasts by protein electrophoresis. Am. J. Enol. Vitic. 38: 49–53
— (1988) Characterization of brewing yeast strains by numerical analysis of total soluble cell protein patterns. S. Afr. J. Enol. Vitic. 94: 245–248
Van Wyk CJ & Pretorius IS (1990) A comparative study of new yeast hybrids. S. Afr. Soc. Enol. Vitic. Cong. 14th, Cape Town
Vezinhet F, Blondin B & Hallet J-N (1990) Chromosomal DNA patterns and mitochondrial DNA polymorphisms as tools for identification of enological strains ofSaccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 32: 568–571
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van der Westhuizen, T.J., Pretorius, I.S. The value of electrophoretic fingerprinting and karyotyping in wine yeast breeding programmes. Antonie van Leeuwenhoek 61, 249–257 (1992). https://doi.org/10.1007/BF00713932
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DOI: https://doi.org/10.1007/BF00713932