Summary
In order to investigate the significance of the histological change in glomerular epithelial cells in minimal change nephrotic syndrome in man (MCNS) and to help in clarifing the mechanism of action of a steroid in this disease, methylprednisolone was administered to rats with puromycin aminonucleoside nephrosis (PAN). This is an experimental nephrosis having a close resemblance morphologically and physiologically, to human MCNS. Morphological changes in the glomerulus were observed ultrastructurally. The administration of the steroid to PAN rats showed remarkable changes including, rapid disappearance of proteinuria in PAN rats in a manner similer to that seen in human MCNS, and significantly faster recovery of changes in glomerular epithelial cells when compared with spontaneous recovery. From the present study, it is clear that the steroid is effective in rapidly restoring the normal shape of glomerular epithelial cells in PAN rats. The filtration barrier in the glomerular capillary wall (GCW) is also thought to have recovered and proteinuria is cured. Based on these considerations, it may be suggested that proteinuria in human MCNS is caused by changes in glomerular epithelial cells, and that the clinical treatment of proteinuria in MCNS is effective when glomerular epithelial cells have functionally recovered.
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References
Andrews PM (1977) A Scanning and transmission electron microscopic comparison of puromycin aminonucleoside-induced nephrosis to hyperalbuminemia-induced proteinuria with emphasis on kidney podocyte pedicle loss. Lab Invest 36:183–197
Andrews PM (1978) Glomerular epithelial alterations resulting from sialic acid surface coat removal. Kid Inter 15:376–385
Bennet CM, Glassock RJ, Chang RLS, Deen WM, Robertson CR, Brenner B (1976) Permselectivity of the glomerular capillary wall. J Clin Invest 57:1287–1294
Bhuyan UN, Welbourn CRB, Evans DJ, Peters TJ (1980) Biochemical studies of the isolated rat glomerulus and the effects of puromycin aminonucleoside aministration. Br J Exp Pathol 61:69–75
Blau EB, Haas JE (1973) Glomerular sialic acid and proteinuria in human renal disease. Lab Invest 28:477–481
Bretton R, Rouchon M, Bariety J (1979) Podocytes in aminonucleoside glomerulonephritis investigated ultrastructurally with Concanavalin A. J Histochem Cytochem 27:1588–1595
Bridges CR, Myers BD, Brenner BM, Deen WM (1982) Glomerular charge alterations in human minimal change nephropathy. Kid Inter 22:677–684
Carrie BJ, Sayler WR, Meyers BD (1981) Minimal change nephropathy. An electrochemical disorder of the glomerular membrane. Am J Med 70:262–268
Caulfield JP, Reid JJ, Farquhar MG (1976) Alterations of the glomerular epithelium in acute aminonucleoside nephrosis. Lab Invest 34:43–53
Chang RLS, Deen WM, Robertson CR, Brenner BM (1975) Permselectivity of the glomerular capillary wall. III. restricted transport of polyanions. Kid Intern 8:212–218
Cole BR, Brocklebank JT, Kienstra RA, Kissane JM, Robson AM (1976) ‘Pulse’methylprednisolone therapy in the treatment of severe glomerulonephritis. J Pediatr 88:307–314
Deen WM, Savat B, Jamieson JM (1980) Theoretical model for glomerular filtration of charged solutes. Am J Physiol 238:F126-F139
Frenk S, Antonowicz I, Craig JM, Metcoff J (1955) Experimental nephrotic syndrome induced in rats by aminonucleoside. Renal lesions and body electrolyte composition. Proc Soc Exp Biol Med 89:424–427
Haynes WDG (1981) The normal human renal glomerulus. Virchows Arch A Cell Pathol 35:133–158
Hoyer JR, Ratte J, Potter AH, Michael AF (1972) Transfer of aminonucleoside nephrosis by renal transplantation. J Clin Invest 51:2777–2780
Kanwar YS, Farquhar MG (1979) Presence of heparan sulfate in the glomerular basement membrane. Proc Natl Acad Sci USA 76:1303–1307
Kanwar YS, Linker A, Farquhar MG (1980a) Increased permeability of glomerular basement membrane to ferritin after removal of glycosaminoglycans (heparan sulfate) by enzyme digestion. J Cell Biol 86:688–693
Kanwar YS, Farquhar MG (1980b) Detachment of endothelium and epithelium from the glomerular basement membrane produced by kidney perfusion with neuraminidase. Lab Invest 42:375–384
Latta H, Johnston WH, Stanley TM (1975) Sialoglycoproteins and filtration barriers in the glomerular capillary wall. J Ultrastr Res 51:354–376
Michael AF, Blau E, Vernier RL (1970) Glomerular polyanion. Alteration in aminonucleoside nephrosis. Lab Invest 23:649–657
Misra RP, Berman LB (1972) Studies on glomerular basement membrane. III. Effects of steroid on membrane chemistry and its protein permeability. Lab Invest 26:666–670
Olson JL, Rennke HG, Venkatachalam MA (1981) Alterations in the charge and size selectivity barrier of the glomerular filter in aminonucleoside nephrosis in rats. Lab Invest 44:271–279
Robson AM, Giangiacomo J, Kienstra RA, Naqvi ST, Ingelfinger JR (1974) Normal glomerular permeability and its modification by minimal change nephrotic syndrome. J Clin Invest 54:1190–1199
Rosenweig LJ, Kanwar YS (1982) Removal of sulfated (heparan sulfate) or nonsulfated (hyaluronic acid) glycosaminoglycans results in increased permeability of the glomerular basement membrane to1311I-bovine serum albumin. Lab Invest 47:177–184
Seiler MW, Rennke HG, Venkatachalam MA, Cotran RS (1977) Pathogenesis of polycation-induced alterations (fusion) of glomerular epithelium. Lab Invest 36:48–61
Studzinski GP, Albanese EA (1980) Nascent RNA chain termination and ultrastructural changes in nucleoli isolated from SV40 transformed fibroblasts treated with aminonucleoside of puromycin. Lab Invest 43:427–433
Webel ML, Donadio JV, Woods JE, Maher FT (1972) Effects of a large dose of methylprednisolone on renal function. J Lab Clin Med 80:765–771
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Fujiwara, Y. An ultrastructural study of the effect of the steroid in puromycin aminonucleoside nephrosis rats. Vichows Archiv A Pathol Anat 405, 11–24 (1984). https://doi.org/10.1007/BF00694922
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DOI: https://doi.org/10.1007/BF00694922