Summary
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1.
The relationship between rate of chloride uptake and external chloride concentration was investigated in Rainbow trout,Salmo gairdneri, and Perch,Perca fluviatilis. The relationship between nitrite uptake and external nitrite and the inhibition of chloride uptake by nitrite was also investigated. Nitrite tolerance tests were performed on a variety of freshwater animals, including Carp,Cyprinus carpio, Tench,Tinca tinca, Pike,Esox lucius, Eel,Anguilla anguilla, and tadpoles,Rana temporaria.
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2.
The chloride uptake mechanism is saturable, with maximum uptake rates of 368 μMh−1kg−1 and 429 μMh−1kg−1 for the trout and perch, respectively. The half saturation value (K m, the affinity constant) is 159 μMl−1 for trout and 333 μMl−1 for perch.
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3.
Net nitrite transport was determined in trout, net movement being into the fish against a concentration gradient, with a maximum uptake rate of 281 μMh−1kg−1; theK m is 198 μMl−1. This suggests that nitrite enters the fish via an active uptake process.
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4.
The data suggest that nitrite is a simple competitive inhibitor of active chloride uptake in both trout and perch. Trout are less tolerant of nitrite than perch (24-h LC50 values are 0.7 mMl−1 for trout and 1.2 mM l−1 for perch) and also have a greater affinity for nitrite.
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5.
The spectrum of nitrite sensitivity seen in freshwater animals is discussed in relation to chloride uptake kinetics. These data support the hypothesis that nitrite uptake is an active process and furthermore uptake is linked quantitatively with chloride uptake, suggesting that chloride and nitrite enter the fish via the same route.
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References
Ainsworth S (1977) Steady state enzyme kinetics. Macmillan Press Ltd., London
Araki T, Ito M, Oscarsson O (1961) Anion permeability of the synaptic and non-synaptic motorneurone membrane. J Physiol 159:410–435
Arillo A, Gaino E, Margiocco C, Mensi P, Schenone G (1984) Biochemical and ultrastructural effects of nitrite on rainbow trout: Liver hypoxia at the root of the acute toxicity mechanism. Environ Res 34:135–154
Bath RN, Eddy FB (1978) Measurement of transbranchial potentials in fish. J Physiol 285:4–5P
Bath RN, Eddy FB (1980) Transport of nitrite across fish gills. J Exp Zool 214:119–121
Bath RN (1980) Some physiological effects of seawater and nitrite on the Rainbow trout (Salmo gairdneri, Richardson). PhD thesis, University of Dundee, Dundee, Scotland
Bielawski J (1964) Chloride transport and water uptake into isolated gills of crayfish. Comp Biochem Physiol 13:423–432
Collins MT, Gratzek JB, Shotts EB, Dane DL, Campbell LM, Senn DR (1975) Nitrification in an aquatic recirculating system. J Fish Res Bd Can 32:2025–2031
Colt J, Tchobanoglous G (1976) Evaluation of the short term toxicity of nitrogenous compounds to channel catfish (Ictalurus punctatus). Aquaculture 8:209–224
Crawford RE, Allen GH (1977) Seawater inhibition of nitrite toxicity to chinook salmon. Trans Am Fish Soc 106:105–109
de Renzis G, Maetz J (1973) Studies on the mechanism of chloride absorption by the goldfish gill: relation with acid-base regulation. J Exp Biol 59:339–358
Dixon M, Webb EC (1964) Enzymes, 2nd edn. Longman, London
Eddy FB, Bath RN (1979) Effects of lanthanum on sodium and chloride fluxes in the Goldfish,Carassius auratus. J Comp Physiol 129:145–149
Eddy FB, Kunzlik PA, Bath RN (1983) Uptake and loss of nitrite from the blood of rainbow trout,Salmo gairdneri Richardson, and Atlantic salmon,Salmo salar L. in freshwater and dilute seawater. J Fish Biol 23:105–116
EIFAC (European Inland Fish Advisory Commission) (1984) Water quality criteria for European freshwater fish. Report on nitrite and freshwater fish. EIFAC Tech Pap 46:19p. FAO, Rome
Forman D, Al-Dabbagh S, Doll R (1985) Nitrates, nitrites and gastric cancer in Great Britain. Nature 313:620–625
Gaino E, Arillo A, Mensi P (1984) Involvement of the gill chloride cells of trout under acute nitrite intoxication. Comp Biochem Physiol 77A:611–617
Heisler N (1984) Acid-base regulation in fish. In: Hoar WS, Randall DJ (eds) Fish physiology, vol XA Ch. 6. Academic Press, New York, pp 315–401
Hoffmann EK (1982) Anion exchange and anion-cation cotransport systems in mammalian cells. Philos Trans R Soc Lond B 299:519–535
Kerstetter TH, Kirschner LB (1972) Active chloride transport by the gills of rainbow trout (Salmo gairdneri) J Exp Biol 56:263–272
Kirsch R (1972) The kinetics of peripheral exchanges of water and electrolytes in the silver eel (Anguilla anguilla L.) in freshwater and sea water. J Exp Biol 57:489–512
Krous SR, Blazer VS, Meade TL (1982) Effect of acclimation time on nitrite movement across the gill epithelia of rainbow trout. The role of “chloride cells”. Prog Fishcult 44:126–130
Margiocco C, Arillo A, Mensi P, Schenone G (1983) Nitrite bioaccumulation inSalmo gairdneri Rich., and haematological consequences. Aquat Toxicol 3:261–270
Maetz J (1956) Les echanges du sodium chez le poissonCarassius auratus L. Action d'un inhibiteur de l'anhydrase carbonique. J Physiol (Paris) 48:1085–1099
Palachek RM, Tomasso JR (1984) Toxicity of nitrite to channel catfish (Ictalurus punctatus), tilapia (Tilapia aurea), and largemouth bass (Micropterus salmoides): Evidence for a nitrite exclusion mechanism. Can J Fish Aquat Sci 41:1739–1744
Perrone SJ, Meade TL (1977) Protective effect of chloride on nitrite toxicity to coho salmon (Oncorhynchus kisutch). J Fish Res Bd Can 34:486–492
Perry SF, Payan P, Girard JP (1984a) Adrenergic control of branchial chloride transport in the isolated perfused head of the freshwater trout (Salmo gairdneri). J Comp Physiol B 154:269–274
Perry SF, Davie PS, Daxboeck C, Ellis AG, Smith DG (1984b) Perfusion methods for the study of gill physiology. In: Hoar WS, Randall DJ (eds) Fish physiology, vol XB, Ch 10. Academic Press, New York, pp 325–388
Potts WTW (1984) Transepithelial potentials in fish gills. In: Hoar WS, Randall DJ (eds) Fish physiology, vol XB, Ch 4. Academic Press, New York, pp 105–130
Russo RC, Thurston RV, Emerson K (1981) Acute toxicity of nitrite to rainbow trout (Salmo gairdneri): effects of pH, nitrite species and anion species. Can J Fish Aquat Sci 38:387–393
Russo RC, Thurston RV (1977) The acute toxicity of nitrite to fishes. In: Tubb RA (ed) Recent advances in fish toxicology. Proc Symp Corvallis, Oregon, US EPA 6Ao/3-77-085
Shechter H, Gruener N, Shuval HI (1972) A micromethod for the determination of nitrite in blood. Anal Chim Acta 60:93–99
Tomasso JR, Simco BA, Davis KB (1979) Chloride inhibition of nitrite induced methaemoglobinemia to channel catfish (Ictalurus punctatus). J Fish Res Bd Can 36:1141–1144
Wedemeger G, Yasutake WT (1978) Prevention and treatment of nitrite toxicity in juvenile steelhead trout (Salmo gairdneri). J Fish Res Bd Can 35:822–827
White RJ (1983) Nitrate in British waters. Aqua 2:51–57
Wolff A, Wasserman AE (1972) Nitrates, nitrites, and nitrosoamines. Science 177:15–19
Wood CM, Wheatly MG, Hōbe HJ (1984) The mechanisms of acid-base and ionoregulation in the freshwater rainbow trout during environmental hyperoxia and subsequent normoxia. III. Branchial exchanges. Resp Physiol 55:175–192
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Williams, E.M., Eddy, F.B. Chloride uptake in freshwater teleosts and its relationship to nitrite uptake and toxicity. J Comp Physiol B 156, 867–872 (1986). https://doi.org/10.1007/BF00694263
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DOI: https://doi.org/10.1007/BF00694263