Abstract
Of 21 recently isolated strains of methanococci, one was found to harbor a small, cryptic, low copy number plasmid. Reproducible recovery was achieved by alkaline lysis of cells pretreated with proteinase K in an osmotically stabilizing buffer. The plasmid was found to contain a singleAval site. No homology was detected between the plasmid and DNA from any of the other new strains or from five known species of methanococci.
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Balch WE, Fox GE, Magrum LJ, Woese CR, Wolfe RS (1979) Methanogens: reevaluation of a unique biological group. Microbiol Rev 43:260–296
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Hintermann G, Fischer H-M, Crameri R, Hutter R (1981) Simple procedure for distinguishing CCC, OC, and L forms of plasmid DNA by agarose gel electrophoresis. Plasmid 5:371–373
Klein A, Schnorr M (1984) Genome complexity of methanogenic bacterial. J Bacteriol 158:628–631
Kleinschmidt AK (1968) Monolayer techniques in electron microscopy of nucleic acids. Methods Enzymol 12:361–377
Langer PR, Waldrop AA, Ward DC (1981) Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78:6633–6637
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Meile L, Keiner A, Leisinger T (1983) A plasmid in the archaebacteriumMethanobacterium thermoautotrophicum. Mol Gen Genet 191:480–484
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Thomm M, Altenbuchner J, Stetter KO (1983) Evidence for a plasmid in a methanogenic bacterium. J Bacteriol 153:1060–1062
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Wood, A.G., Whitman, W.B. & Konisky, J. A newly-isolated marine methanogen harbors a small cryptic plasmid. Arch. Microbiol. 142, 259–261 (1985). https://doi.org/10.1007/BF00693400
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DOI: https://doi.org/10.1007/BF00693400