Summary
Using the brains of 30 patients with mental and neurologic disorders, we studied the intracytoplasmic acidophilic granules in neurons of the substantia nigra and locus ceruleus by light and electron microscopy. The granules were present in all 30 brains, including those with no recognizable pathologic change, there was no correlation between their appearance and the age, sex, disease of, or the medication received by, the patients. In four electron-microscopically examined brains, we noted many small, round electron-dense bodies in the perikarya and neuronal processes of the substantia nigra and locus ceruleus. The bodies were packed tightly within a double membrane; in shape, size, and distribution in the neuronal cytoplasm, they corresponded to acidophilic granules. Some mitochondrial matrices contained one or more similar, but smaller inclusion bodies; larger bodies pushed aside the mitochondrial cristae. We conclude that the acidophilic granules represent highly developed forms of mitochondrial inclusions.
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Beaver DL, Moses HL, Ganote CE (1965) Electron microscopy of the trigeminal ganglion. II. Autopsy study of human ganglia. Arch Pathol 79:557–570
Chang SC (1972) Hematoxylin-eosin staining of plastic-embedded tissue sections. Arch Pathol 93:344–351
Earle KM (1968) Studies on Parkinson's disease including X-ray fluorescent spectroscopy of formalin fixed brain tissue. J Neuropathol Exp Neurol 27:1–14
Foley JM, Baxter D (1958) On the nature of pigment granules in the cells of the locus coeruleus and substantia nigra. J Neuropathol Exp Neurol 17:586–598
Forno LS, Norville RL (1975) Ultrastructural studies of the human locus ceruleus (in middle-aged and older persons with and without parkinsonism). In: Proceedings of the VIIth International Congress Neuropathology. Excerpta Medica, Amsterdam Akademia Imiado, Budapest, pp 459–462
Forno LS, Norville RL (1976) Ultrastructure of Lewy bodies in the stellate ganglion. Acta Neuropathol (Berl) 34:183–197
Hirosawa K (1968) Electron microscopic studies on pigment granules in the substantia nigra and locus coeruleus of the Japanese monkey (Macaca fuscata yakui). Z Zellforsch 88:187–203
Issidorides MR, Mytillineou C, Whetsell WO Jr, Yahr MD (1978) Protein-rich cytoplasmic bodies of substantia nigra and locus ceruleus. A comparative study in Parkinsonian and normal brain. Arch Neurol 35:633–637
Lillie RD, Yamada H (1960) Histochemical studies on the neuromelanins. Okajima Folia Anat Jpn 36:155–163
Lipkin L (1959) Cytoplasmic inclusions in gagnlion cells associated with parkinsonian states. A neurocellular change studied in 53 cases and 206 controls. Am J Pathol 35:1117–1133
Marinesco MG (1902) Sur la présence des corpuscules acidophiles paranucléolaires dans les céllules du locus niger et du locus coeruleus. C R Acad Sci 135:1000–1002
Moses HL, Beaver DL, Ganote CE (1965) Electron microscopy of the trigeminal ganglion. I. Comparative ultrastructure. Arch Pathol 79:541–556
Ohsuga N, Shionoya S, Kamiya K, Hoshino M (1966) An electron microscopic study of the human sympathetic ganglion cell. I. Inclusion bodies. J Electron Microse (Tokyo) 15:26–27
Pick J, DeLemos C, Gerdin C (1964) The fine structure of sympathetic neurons in man. J Comp Neurol 122:19–67
Pick J (1967) Pigment, abnormal mitochondria and laminar bodies in human sympathetic neurons. An electron microscopic study. Z Zellforsch 82:118–135
Schochet SS Jr, Wyatt RB, McCormick WF (1970) Intracytoplasmic acidophilic granules in the substantia nigra. A light and electron microscopic study. Arch Neurol 22:550–555
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Sekiya, S., Tanaka, M., Hayashi, S. et al. Light- and electron-microscopic studies of intracytoplasmic acidophilic granules in the human locus ceruleus and substantia nigra. Acta Neuropathol 56, 78–80 (1982). https://doi.org/10.1007/BF00691186
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DOI: https://doi.org/10.1007/BF00691186