Summary
Nine dogs with canine distemper encephalitis (CDE) were examined with immunological techniques including demonstration of antibodies against canine distemper virus (CDV) in the serum and against myelin basic protein (MBP) in serum and in CSF. Mitogen stimulation tests of lymphocytes were also done. The brains were examined pathologically and immunoglobulin and C3 were demonstrated in lesions by means of immunohistological techniques.
Six dogs with acute CDE had none or low antibody levels against CDV or MBP, and there was no immunoglobulin in demyelinating lesions. Some of these dogs had depressed lymphocyte mitogen responses. Two dogs with chronic CDE showed recovery of lymphocyte mitogen responses. One of these had a significant antibody response against CDV and MBP in the serum. Both dogs with chronic CDE had very high antibody titers against MBP in the CSF and demyelinating lesions contained immunoglobulin. These results suggest that acute demyelination in CDE is probably due to some direct viral activity and that the progression of demyelination in chronic CDE is associated with a local immune response.
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References
Appel MJG, Gillespie JH (1972) Canine distemper virus. Virology monographs. Springer, Wien New York
Appel MJG, Robson DS (1973) A microneutralization test for canine distemper virus. Am J Vet Res 34:1459–1463
Brosnan CF, Stoner GL, Bloom BR, Wisniewski HM (1977) Studies on demyelination by activated lymphocytes in the rabbit eye. II. Antibody-dependent cell-mediated demyelination. J Immunol 118:2103–2110
Fankhauser R (1962) The cerebrospinal fluid. In: Innes JRM, Saunders LZ (eds) Comparative neuropathology. Academic Press. New York London
Golds EE, Braun P (1978): Cross-linking studies on the conformation and dimerisation of myelin basic protein in solution. J Biol Chem 253:8171–8177
Grundke-Iqbal I, Lassmann H, Wisniewski HM (1980) Chronic relapsing experimental allergic encephalomyelitis. Arch Neurol 37:651–656
Higgins RJ, Krakowka S, Koestner A (1982a) Acute canine distemper virus induced encephalomyelitis in gnotobiotic dogs—A sequential ultrastructural study. (Submitted) Acta neuropathol (Berl)
Higgins RJ, Krakowka S, Metzler AK, Koestner A (1982b) Primary demyelination in CDV-induced encephalomyelitis in gnotobiotic dogs. (Submitted) Acta Neuropathol (Berl)
Ho CK, Babiuk LA (1979) Immune mechanisms against canine distemper. I. Identification of K cell against canine distemper virus-infected target cells in vitro. Immunology 37:231–239
Itoyama Y, Sternberger NH, Kies MW, Cohen SR, Richardson EP Jr, Webster H de F (1980a) Immunocytochemical, method to identify myelin basic protein in oligodendroglia and myelin sheaths of the human nervous system. Ann Neurol 7:157–166
Itoyama Y, Sternberger NH, Webster H de F, Quarles RH, Cohen SR, Richardson EP, Jr (1980b) Immunocytochemical observations on the distribution of myelin-associated glycoprotein and myelin basic protein in multiple selerosis lesions. Ann Neurol 7:167–177
Imagawa DT (1968) Relationship among measles, canine distemper, and Rinderpest viruses. Prog Med Virol 10:160–193
Koestner A, McCullough B, Krakowka GS, Long JF, Olsen RG (1974) Canine distemper, a virus-induced demyelinating encephalomyelitis. In: Zeman W, Lenette EH (eds) Slow virus diseases. Williams and Wilkins, Baltimore, pp 86–101
Koestner A (1975) Animal model of human disease. Subacute sclerosing panencephalitis, multiple sclerosis. Am J Pathol 78:361–364
Krakowka S, McCullough B, Koestner A, Olsen RG (1973) Myelinspecific autoantibodies associated with central nervous system demyelination in canine distemper, virus infection. Infect Immun 8:819–827
Krakowka S, Higgins RJ, Koestner A (1980) Canine distemper virus: Review of structural and functional modulations in lymphoid tissues. Am J Vet Res 41:284–292
Krakowka S, Wallace AL, Koestner A (1981) Shared antigenic determinants between brain and thymus-derived lymphocytes in dogs. Implications regarding the significance of antimyelin antibodies in demyelination. Acta Neuropathol (Berl) 54:75–82
Kristensen B, Kristensen F, Vandevelde M, Higgins RJ, de Weck AL (1982a): Canine lymphocyte cultures in vitro: Evaluation of peripheral blood lymphocyte response to mitogens (Submitted) Vet Immunol Immunopathol
Kristensen F, Kristensen B, Vandevelde M, Higgins RJ, de Weck AL (1982b): Analysis of the in vitro activation and proliferation process in lymphocytes deriving from canine thymus and mesenteric lymph node. (Submitted) Vet Immunol Immunopathol
Kristensson K, Thormar H, Wisniwski HM (1979) Myelin lesions in the rabbit eye model as a bystander effect of herpes simplex and visna virus sensitization. Acta Neuropathol (Berl) 48:215–217
Lassmann H, Wisniewski HM (1978) Chronic relapsing EAE. Time course of neurological symptoms and pathology. Acta Neuropathol (Berl) 43:35–42
Martin JR, Nathanson N (1979) Animal models of virus-induced demyelination. In: Zimmerman HM (ed) Progress in neuropathology, vol 4. Raven Press, New York, pp 27–50
McCullough B, Krakowska S, Koestner A (1974) Experimental canine distemper virus-induced lymphoid depletion. Am J Pathol 74:155–165
Moulton JE (1956) Fluorescent antibody studies of demyelination in canine distemper. Proc Soc Exp Biol 91:460–464
Raine CS (1976) On the development of CNS lesions in natural canine distemper encephalomyelitis. J Neurol Sci 30:13–28
Shek WR, Schultz RD, Appel MJ (1980): Natural and immune cytolysis of canine distemper virus-infected target cells. Infect Immun 28:724–734
Steck AJ, Tschannen R, Schaefer R (1981) Induction of antimyelin and antioligodendrocyte antibodies by vaccinia virus: experimental study in the mouse. J Neuroimmun 1:117–124
Sternberger LA (1979) Immunocytochemistry, 2nd edn. Wiley & Sons, New York Chichester Brisbane Toronto, pp 122–129
Summers BA, Greisen HA, Appel MJG (1979) Early events in canine distemper demyelinating encephalomyelitis. Acta Neuropathol (Berl) 46:1–10
Traugott U, Raine CS (1979) Acute experimental allergic encephalomyelitis. Myelin basic protein-reactive T cells in the circulation and in meningeal infiltrates. J Neurol Sci 42:331–336
Vandevelde M, Kristensen B (1977) Observations on the distribution of canine distemper virus in the central nervous system of dogs with demyelinating encephalitis. Acta Neuropathol (Berl) 40:233–236
Vandevelde M, Spano JS (1977) Cerebrospinal fluid cytology in canine neurologic disease. Am J Vet Res 38:1827–1832
Vandevelde M, Fankhauser R, Kristensen F, Kristensen B (1981) Immunoglobulins in demyelinating lesions in canine distemper encephalitis. Acta Neuropathol (Berl) 54:31–41
Wisniewski HM, Brosnan CF, Bloom BR (1980) Bystander and antibody-dependent cell-mediated demyelination. In: Davison AN, Cuzner ML (eds) The suppression of experimental allergic encephalomyelitis and multiple sclerosis. Academic Press, London New York Toronto Sydney San Francisco, pp 45–57
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Supported by the Schweizer Nationalfonds grands nos. 3.805.79 (to M. V.) and 3.957-0.80 (to A. J. S.) and the Schweizerische Multiple Sklerose Gesellschaft
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Vandevelde, M., Kristensen, F., Kristensen, B. et al. Immunological and pathological findings in demyelinating encephalitis associated with canine distemper virus infection. Acta Neuropathol 56, 1–8 (1982). https://doi.org/10.1007/BF00691174
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DOI: https://doi.org/10.1007/BF00691174