Summary
Heat death and resistance adaptation of freshwater crayfish are thought to be properties of its muscle membranes. The inactivation at high temperatures of a membrane-bound enzyme, the Ca++-stimulated ATPase of crayfish abdominal muscle sarcoplasmic reticulum, and the effect of thermal acclimation of crayfish upon the inactivation kinetics have been investigated. In the absence of KCl, the Ca++-stimulated ATPase is irreversibly inactivated with pseudo-first order kinetics at temperatures that cause heat death in the whole animal. 0.1–10.0 mM KCl resulted in slower inactivation, while 100 mM KCl activated the enzyme to 120–180% of its original activity. Enzyme activation by KCl and heat involved a shift in the enzyme concentration/activity curve. Thermal acclimation of crayfish had no significant effect upon the kinetics or Arrhenius activation energy for enzyme inactivation (100.6±10.5 and 92.3±14.6 kcal/mole for preparations from 4°C and 25°C acclimated crayfish).
Ca++-stimulated ATPase isolated from heat dead crayfish exhibited normal in vitro activity due presumably to the high intracellular K+ concentration. Nevertheless, the close correspondence between heat death temperatures and inactivation temperatures for several membrane-bound enzymes of muscle is thought to reflect some perturbation of muscle structure that occurs during heat death.
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Abbreviations
- ATP:
-
Ademosine 5′-Triphosphate
- EGTA:
-
Ethyleneglycol-bis [β-amino-ethyl ether]
- N:
-
N′-tetraacetic acid
- Hepes:
-
N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acid
- FSR:
-
Fragmented sarcoplasmic reticulum
- Tris:
-
Tris (hydroxymethyl)aminomethane
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Cossins, A.R., Bowler, K. Resistance adaptation of the freshwater crayfish and thermal inactivation of membrane-bound enzymes. J Comp Physiol B 111, 15–24 (1976). https://doi.org/10.1007/BF00691107
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DOI: https://doi.org/10.1007/BF00691107