Summary
Serotonin (5-HT)-like immunoreactive nerve fibers were investigated in gut tissue obtained from seven Japanese children with Hirschsprung's disease. In the control untreated tissues, 5-HT-like immunoreactive fibers were observed neither in the normoganglionic nor in the aganglionic regions. After pargyline treatment, 5-HT-positive neuropils were consistently detected in association with the myenteric plexus in the normoganglionic segment, while in the aganglionic segment immunoreactive fibers could not be demonstrated through the entire layer of the bowel tissue. The occurence of 5-HT-like immunoreactive neuropils by pargyline treatment strongly suggests that the infant bowel is innervated with serotoninergic elements. After treatment with 5-hydroxy-l-tryptophan (5-HTP), the immunoreactive neuropils were localized in the myenteric and submucous plexuses of the normoganglionic segment. On the other hand, another type of 5-HT-positive fibers was characteristically demonstrated in aganglionic segments following the drug treatment; moderate numbers of 5-HT-like immunoreactive fibers appeared in the intermuscular zone and within the circular and longitudinal muscle layers. Several 5-HT-positive fibers were present in the hypertrophic nerve bundles seen in a diseased bowel. A possible origin of this type of 5-HT-positive nerve fibers was discussed.
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Costa M, Furness JB, Cuello AC, Verhofstad AAJ, Steinbusch HWJ, Elde RP (1982) Neurons with 5-hydroxytryptamine-like immunoreactivity in the enteric nervous system: Their visualization and reactions to drug treatment. Neuroscience 7:351–363
Dreyfus CF, Sherman DL, Gershon MD (1977) Uptake of serotonin by intrinsic neurons of the myenteric plexus grown in organotypic tissue culture. Brain Res 128:109–123
Frigo GM, Del Tacca M, Lecchini S, Crema A (1973) Some observations on the intrinsic nervous mechanism in Hirschsprung's disease. Gut 14:35–40
Furness JB, Costa M (1978) Distribution of intrinsic nerve cell bodies and axons which take up aromatic amines and their precursors in the small intestine of the guinea pig. Cell Tissue Res 188:527–543
Gershon MD, Dreyfus CF, Pickel VM, Joh TH, Reis DJ (1977) Serotonergic neurons in the peripheral nervous system: Identification in gut by immunohistochemical localization of tryptophan hydroxylase. Proc Natl Acad Sci USA 74: 3086–3089
Gonella J (1981) The physiological role of peripheral serotoninergic neurons. A review. J Physiol (Paris) 77:515–519
Griffith SG, Burnstock G (1983) Serotoninergic neurons in human fetal intestine: An immunohistochemical study. Gastroenterology 85:929–937
Grota LJ, Brown GM (1974) Antibodies to indolealkylamines: Serotonin and melatonin. Can J Biochem 52:196–202
Hsu S-M, Raine L, Fanger H (1981) Use of avidin-biotinperoxidase complex (ABC) in immunoperoxidase techniques. J Histochem Cytochem 29:577–580
Ikeda K (1967) New techniques in the surgical treatment of Hirschsprung's disease. Surgery 61:503–508
Kurian SS, Ferri G-L, De Mey J, Polak JM (1983) Immunocytochemistry of serotonin-containing nerves in the human gut. Histochemistry 78:523–529
Nado O, Hiratsuka T, Komatsu K (1984) The occurrence of serotonin-containing cells in the esophageal epithelium of the bullfrogRana catesbiana: A fluorescence histochemical and immunohistochemical study. Histochemistry 81:115–118
Richardson J (1975) Pharmacological studies of Hirschsprung's disease on a murine model. J Pediatr Surg 10:875–884
Robinson RG, Gershon MD (1971) Synthesis and uptake of 5-hydroxytryptamine by the myenteric plexus of the guineapig ileum: A histochemical study. J Pharmacol Exp Ther 178:311–324
Rogawski MA, Goodrich JT, Gershon MD, Touloukian RJ (1978) Hirschsprung's disease: Absence of serotonergic neurons in the aganglionic colon. J Pediatr Surg 13:608–615
Snipes RL, Thoenen H, Tranzer JP (1968) Fine structural localization of exogenous 5-HT in vesicles of adrenergic nerve terminals. Experientia (Basel) 24:1026–1027
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Toyohara, T., Nada, O., Nagasaki, A. et al. An immunohistochemical study of serotoninergic nerves in the colon and rectum of children with Hirschsprung's disease. Acta Neuropathol 68, 306–310 (1985). https://doi.org/10.1007/BF00690833
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DOI: https://doi.org/10.1007/BF00690833