Abstract
A technique was developed for synchronization ofHyphomicrobium sp. strain B-522. Bacteria were grown in continuous culture with methanol (0.1%; v/v) growth limiting. Vitamin B12 (2.5 μg/l) was necessary to obtain steady state growth. The critical dilution rate wasD c =0.112; maximum cell output occurred atD=0.105 (Dx=30 mg l-1 h-1). Continuous cultures ofHyphomicrobium B-522 atD=0.110 were used to obtain cells for synchronization experiments. Synchronization was achieved by trapping young hyphal and budding cells in a glass wool column, while the initial swarmer cells were allowed to pass through. By semicontinuously rinsing the system, newly produced swarmers could be sampled in the effluent. The mean length of these synchronous swarmer cells was 1.25 μm (s=±0.13 μm; range 0,6 μm) as compared to 1.40 μm (s=±0.21 μm; range 1.2 μm) for swarmer cells of the continuous culture. Division of synchronous swarmer populations was completed after 7 h; the synchronization index was 0.76.
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References
Dwerk ED, Kobrin LH, Grossman N, Ron RZ (1980) Synchronization of cell division in microorganisms by percoll gradients. J Bacteriol 144:17–21
Herbert D, Elsworth R, Telling RC (1956) The continuous culture of bacteria; a theoretical and experimental study. J Gen Microbiol 14:601–622
Hirsch P (1974a) Budding and/or appendaged bacteria. In: RE Buchanan, NE Gibbons (eds) Bergey's manual of determinative bacteriology, 8th ed. The Williams and Wilkins Co, Baltimore, pp 148–151
Hirsch P (1974b) Budding bacteria. Ann Rev Microbiol 28:391–444
Hirsch P, Conti SF (1964a) Biology of budding bacteria I. Enrichment, isolation and morphology ofHyphomicrobium spp. Arch Mikrobiol 48:339–357
Hirsch P, Conti SF (1964b) Biology of budding bacteria II. Growth and nutrition ofHyphomicrobium spp. Arch. Mikrobiol 48:358–367
Hirsch P, Jones HE (1968) DNA location, hyphal growth and life cycle of a budding bacterium,Hyphomicrobium spec., strain B-522. Bacteriol Proc G 149
Matzen N, Hirsch P (1982) Improved growth conditions forHyphomicrobium. sp. B-522 and two additional strains. Arch Microbiol 131:32–35
Moore RL, Hirsch P (1973) First generation synchrony of isolatedHyphomicrobium swarnmer populations. J Bacteriol 116:418–423
Scherbaum OH (1964) Comparison of synchronous and synchronized cell division. Exp Cell Res 33:89–98
Tempest DW (1970) The cultivation of microorganisms 1. Theory of the chemostat. In: JR Norris, DW Ribbons (eds) Methods in microbiology, Vol 2. Academic Press, London New York, pp 259–276
Wali TM, Hudson GR, Danald DA, Weiner RM (1980) Timing of swarmer cell cycle morphogenesis and macromolecular synthesis byHyphomicrobium neptunium in synchronous culture. J Bacteriol 144:406–412
Whittenbury R, Dow CS (1977) Morphogenesis and differentiation inRhodomicrobium vannielii and other budding and prosthecate bacteria. Bacteriol Rev 41:754–808
Wilkinson TG, Hamer G (1972) Some growth characteristics of aHyphomicrobium sp in batch culture. J Appl Bacteriol 35:577–588
Wolosker HBM, de Almeida DF (1979) Quantitative assessment of the synchronization of cell populations. J Gen Microbiol 110:225–227
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Matzen, N., Hirsch, P. Continuous culture and synchronization ofHyphomicrobium sp. B-522. Arch. Microbiol. 132, 96–99 (1982). https://doi.org/10.1007/BF00690826
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DOI: https://doi.org/10.1007/BF00690826