Summary
The distribution of DNA is estimated from flow cytometric histograms in surgical specimens of ten ependymomas of different location and varying anaplasia. In three cerebral tumours grade I–II, including one ependymoma of the 4th ventricle, only limited elevation of the 4 C maxima was a prominent feature, corresponding to the microscopical frequency of typical mitoses. Four grade-III ependymomas showed aneuploid or polyploid histograms with stem lines. One frontal tumour was classified as “transitional” because of more numerous mitoses and abnormally elevated S and G2+M phases, which increased in tissue culture. A correlation between the degree of anaplasia with the DNA pattern was difficult to pursue in two spinal ependymomas obviously lacking microscopical mitoses: Both — one a so-called tanycytic variant of grade I–II, and the other probably a metastasis from a cerebellar tumour — had a clear polyploid DNA histogram with a strikingly increased proliferation index, similar to the more malignant tumours of grade III. Also flow DNA measurements probably allow the decoding of heterogenous mixtures of tumour cells which are not always benign in ependymomas of lower grades of anaplasia microscopically.
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References
Ahyai A, Zimmermann A, Spaar FW 1983. Flow fluorescence-cytometry of deoxyribonucleic acid in meningiomas; studies on surgically removed tumor specimens compared with their cells in primary tissue cultures. Surg Neurol 20:196–205
Arendt A (1974) Ependymomas. In: Vinken PJ,Bruyn GW (eds) Tumors of the brain and skull. Handbook of Clinical Neurology, vol 16. North Holland. Amsterdam, pp 105–150
Baisch H, Göhden W, Linden WA (1975) Analysis of PCP data to determine the fraction of cells in the various phase of cell cycle. Radiat Environ Biophys 12:31–39
Böhm N, Sandritter W (1975) DNA in human tumors: A cytophotometric study. Curr Top Pathol 60:152–219
Dittrich W, Göhde W (1969) Impulscytophotometrie bei Einzelzellen in Suspensionen. Z Naturforsch 245:360
Feichter G, Schwecheimer K, Goertler K (1983) Assistierende impulszytometrische Beurteilung bei der Routine-Diagnostik von Meningeomen und Gliomen. Pathologe 4: 294–302
Fokes EC, Earle KM (1969) Ependymomas; clinical and pathological aspects. J Neurosurg 30:585–594
Friede RL, Pollak A (1978) The cytogenetic basis for classifying ependymomas. J Neuropathol Exp Neurol 37:103–118
Henschen F (1955) Das Ependymom. Tumoren des Zentralnervensystems und seiner Hüllen. In: Scholz W (Hrsg) Hdb Spez Pathol Anat Histol von Lubarsch O, Henke F, Rössle R. Nervensystem, Bd 13/Teil 3, Springer, Berlin Göttingen Heidelberg, S 590–596
Lehmann J, Krug H (1980) Flow-through fluorocytophotometry of different brain tumors. Acta Neuropathol (Berl) 49:123–132
Müller W, Brämisch R, Afra D, Schwenzfeger A (1977) Cytometrische Messungen des DNS-Gehaltes in Ependymomen und Plexuspapillomen. Acta Neuropathol (Berl) 39:255–259
Pia HW (1977) Die Hirngliome. Dtsch Ärztebl 74:1189–1192
Ringertz N, Reymond A (1949) Ependymomas and choroid plexus papillomas. J Neuropathol Exp Neurol 8:355–380
Rubinstein LJ (1972) Tumors of the central nervous system. Atlas of tumor pathology, fasc 6/2nd edn. Armed Forces Institute of Pathology, Washington DC, pp 55–104
Russell DS, Rubinstein LJ (1977) Pathology of tumours of the nervous system, 4th edn. Arnold, London
Zülch KJ (1956) Biologie und Pathologie der Hirngeschwülste. In: Olivecrona H, Tönnis W (Hrsg) Handbuch für Neurochirurgie, Bd 3. Springer, Berlin Göttingen Heidelberg, pp 1–702
Zülch KJ (1958) Die Hirngeschwülste. Barth, Leipzig, pp 129–135
Zülch KJ (1979) Histological typing of the central nervous system. International Histological Classification of Tumours, no. 21. WHO, Geneva
Zülch KJ, Kleinsasser C (1957) Ortsgebundene Abweichungen in der Histologie und im biologischen Verhalten der Ependymome. Zentral Allg Pathol 97:59–66
Zülch KJ, Mennel HD (1974) The biology of brain tumors. In: Vinken PJ, Bruyn GW (eds) Handbook of Clinical Neurology, vol 16. North Holland. Amsterdam, pp 1–55
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Spaar, F.W., Blech, M. & Ahyai, A. DNA-flow fluorescence — Cytometry of ependymomas. Acta Neuropathol 69, 153–160 (1986). https://doi.org/10.1007/BF00687052
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DOI: https://doi.org/10.1007/BF00687052