Summary
A human oligodendroglial cell line has been established from a mixed glioma. Over a period of 22 months, the cell consisted of cells with the morphologic features of such a strong perinuclear halo, dichotomous branching, and a tendency to aggregate and float in the medium. In small aggregates, most cells are bipolar. The cell line also continues to synthetize the S-100 protein specific to neural tissue. The response of this cell line to dibutyryl-cyclic AMP is striking, resulting in a remarkable narrowing and elongation of the cell processes.
This cell line could be used for studies on glial differentiation and neuron-glia interaction.
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Benda, P., Lightbody, J., Sato, G., Levine, L., Sweet, W.: Differentiated rat glial strain in tissue culture. Science161, 370–371 (1968)
Bignami, A., Eng, L. F., Dahl, D., Uyeda, C. T.: Localisation of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res.43, 429–435 (1972)
Edström, A., Kanje, M., Walum, E.: Effects of dibutyryl cyclic AMP and prostaglandin E1 on cultured human glioma cells. Exp. Cell Res.85, 217–223 (1974)
Eng, L. F., Vanderhaeghan, J. J., Bignami, A., Gerstle, B.: An acidic protein isolated from fibrous astrocyte. Brain Res.28, 351–354 (1971)
Haglid, K., Carlsson, C.-A., Stavrou, D.: An immunological study of human brain tumors concerning the brain specific proteins S-100 and 14.3.2. Acta Neuropathol. (Berl.)24, 187–196 (1973)
Hsie, A. W., Puck, T. T.: Morphological transformation of Chinese hamster cells by dibutyryl adenosine cyclic 3′∶5′-monophosphate and testosterone. Proc. Natl. Acad. Sci. USA68, 358–361 (1971)
Johnson, G. S., Friedman, R. M., Pastan, I.: Restoration of several morphological characteristics of normal fibroblasts in sarcoma cells treated with adenosine 3′,5′-cyclic monophosphate and its derivatives. Proc. Natl. Acad. Sci. USA68, 425–429 (1971)
Kersting, G.: Tissue culture of human gliomas. Progr. Neurol. Surg.2, 165–202 (1968)
Liss, L.: The use of tissue culture in the study of brain tumors. Prog. Exp. Tumor Res.17, 93–110 (1972)
Lumsden, C. E., Pomerat, C. M.: Normal oligodendrocytes in tissue culture. A preliminary report on the pulsatile glial cells in tissue cultures from the corpus callosum of the normal adult rat brain. Exp. Cell Res.2, 103–114 (1951)
Manuelidis, E. E.: Long-term lines of tissue cultures of intracranial tumors. J. Neurosurg.22, 368–373 (1965)
Manuelidis, E. E.: Experiments with tissue culture and heterologous transplantation of tumors. Ann. NY Acad. Sci.159, 409–431 (1969)
Moonen, G., Cam, Y., Sensenbrenner, M., Mandel, P.: Variability of the effects of serum-free medium, dibutyryl-cyclic AMP or theophyllin on the morphology of cultured newborn rat astroblasts. Cell Tissue Res.163, 365–372 (1975)
Moore, B. W.: A soluble protein characteristics of the nervous system. Biochem. Biophys. Res. Commun.19, 739–744 (1965)
Mori, T.: Studies on astrocyte-specific antigen (astroprotein). Neurochemistry (Tokyo)9, 75–79 (1970)
Nakai, J., Okamoto, M.: Identification of neuroglial cells in tissue culture. Morphology of neuroglia, pp. 65–102. Springfield, IL: Thomas 1963
Nakazawa, T.: Biological response of oligodendrocyte and astrocyte in tissue culture. Morphology of neuroglia, pp. 103–120. Springfield, IL: Thomas 1963
Ouchterlony, O.: Diffusion in gel methods for immunological analysis. Prog. Allergy5, 1–78 (1958)
Pfeiffer, S. E., Wechsler, W.: Biochemically differentiated neoplastic clone of Schwann cells. Proc. Natl. Acad. Sci. USA69, 2885–2889 (1972)
Pontén, J., Macintyre, E. H.: Long-term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand.74, 465–486 (1968)
Prasad, K. N., Hsie, A. W.: Morphologic differentiation of mouse neuroblastoma cells induced in vitro by dibutyryl adenosine 3′,5′ cyclic monophosphate. Nature233, 141–142 (1971)
del Rio Hortega, P.: Estudios sobre la glia de escasas radiaciones (oligodendroglia). Bol. Real Soc. Espan Hist. Nat.21, 63–92 (1921)
Russel, D. S., Rubinstein, L. J.: Pathology of tumors of the nervous system. 4th ed., pp. 195–203. London: Arnold 1977
Unterharnsheidt, F. J.: Routine tissue culture of CNS tumors and animal implantation. Prog. Exp. Tumor Res.17, 111–150 (1972)
Westermark, B., Pontén, J., Hugosson, R.: Determinants for the establishment of permanent tissue culture lines from human gliomas. Acta Pathol. Microbiol. Scand. [A]81, 791–805 (1973)
Wilson, C. B., Barker, M., Slagel, D. E.: Tumors of the central nervous system in monolayer tissue. Arch. Neurol.15, 275–282 (1966)
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This work was supported by a Grant-in Aid for Scientific Research from the Japan Ministry of Education, Science, and Culture
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Miyake, E. Establishment of a human oligodendroglial cell line. Acta Neuropathol 46, 51–55 (1979). https://doi.org/10.1007/BF00684804
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DOI: https://doi.org/10.1007/BF00684804