Summary
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1.
Larvae of the American toad (Bufo americanus) preferentially associate with their siblings in laboratory tests, suggesting that they can recognize kin. The sensory basis of their kin recognition abilities was investigated by measuring the responses of individuals in a Y-maze to waterborne cues emanating from their siblings and from nonsiblings.
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2.
When simultaneously presented with water flowing from two containers, each holding members of a different sibling group, test subjects spent significantly more time oriented toward their siblings than toward non-siblings. Similar results were obtained when the stimulus water was first passed through intermediary reservoirs. Hence, kinship cues are unlikely to be acoustic or vibratory stimuli perceived by the auditory or lateral line systems.
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3.
Tadpoles whose external nares were blocked with a gelatinous paste did not behaviorally discriminate between water flowing from siblings and that flowing from non-siblings. Retested after their nares were unplugged, these individuals oriented significantly toward their siblings, as did shamtreated test individuals.
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4.
Stimulus water conditioned by sibling groups and then stored for 24–30 h failed to elicit a discrimination response, indicating that kinship cues released by tadpoles lose their effectiveness during this period. Signals with rapid fade-out times would probably be more efficient under natural conditions than those that persisted after individuals had moved.
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5.
Test subjects simultaneously presented with water flowing from siblings and blank (dechlorinated tap) water showed no tendencies to discriminate between these stimuli. When individuals were exposed both to water from non-siblings and to blank water, however, they oriented significantly toward the blank water. Kin association may thus result in part from negative klinokinetic responses induced by contact with factors released by nonkin.
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6.
Chemical cues released by tadpoles appear sufficient to communicate their kinship identity. These cues are probably perceived and processed by the main olfactory system. Kin recognition mechanisms may be further elucidated by chemical and neurophysiological analyses.
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Waldman, B. Olfactory basis of kin recognition in toad tadpoles. J. Comp. Physiol. 156, 565–577 (1985). https://doi.org/10.1007/BF00619107
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DOI: https://doi.org/10.1007/BF00619107