Summary
Amipramizide is capable of inhibiting active sodium transport by isolated bladder, colon and skin of the toad,Bufo marinus, when added in small amounts (⩽10−5 M) to the solution coming in contact with the outer surface of the preparations. Inhibition occurs rapidly, is proportionate to dose and is reversible. No significant effect results from the presence of large amounts of the drug in the solution bathing the inner surface of the membranes.
From measurements of “active sodium transport pool” in toad bladder tissue, it appears that pool size was decreased in proportion to sodium transport; on the other hand, amphotericin B eliminated the action of amipramizide on sodium transport. Therefore amipramizide is assumed to act by impeding the combination of sodium ions with carriers (“permeases”) whose presence is postulated at the apical border of these biological membranes.
Amipramizide did not prevent the preparations from reacting to aldosterone, insulin or vasopressin by increased sodium transport provided the latter had not been suppressed by the drug. With respectto the permeability of toad bladder to water, it was not modified by this drug either.
Amipramizide could be recovered chemically unaltered from incubation medium and from tissue at the end of the experiments.
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Supported finacially by the Fonds de la Recherche Scientifique Médicale (grant no 840), Belgium.
On leave of absence from the University of Chicago, Department of Medicine (Recipient of U.S.P.H.S. Career Development Award K2-GM-21.842).
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Ehrlich, E.N., Crabbé, J. & Scarlata, J. The mechanism of action of amipramizide. Pflugers Arch. 302, 79–96 (1968). https://doi.org/10.1007/BF00586783
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DOI: https://doi.org/10.1007/BF00586783