Abstract
We present a genomic map of infectious laryngotracheitis virus (ILT) and an 18,912 bp sequence containing the entire unique short region and a portion of the flanking short repeats. In determining the genomic map, an 856 bp region repeated as many as 13 times was identified within the short repeats. The unique short sequence contains nine potential open reading frames (ORFs). Six of these ORFs show homology to other known herpesvirus unique short genes. Using the herpes simplex virus nomenclature, these genes are the US2, protein kinase, and glycoproteins G, D, I, and E (ORF 1, 2, 4, 6, 7, and 8, respectively). Interestingly, an open reading frame with homology to HSV-1 UL47 (ORF 3) is found in the unique short. One very large open reading frame (ORF 5) is present and contains a threonine-rich, degenerate repeat sequence. This gene appears to be unique to ILT among sequenced herpesviruses. Two ORFs were identified within the short repeat (SR) region. SRORF 1 is homologous to a gene (SORF3) found in the unique short region in both MDV and HVT, and appears to be specific to avian herpesviruses. SRORF 2 has homology to HSV US10.
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LeibD.A., BradburyJ.M., GaskellR.M., HughesC.S., and JonesR.C., Avian Dis30, 835–837, 1986.
HughesC.S., WilliamsR.A., GaskellR.M., JordanF.T.W., BradburyJ.M., BennettM., and JonesR.C., Arch Virol121, 213–218, 1991.
RoizmannB., DesrosiersR.C., FleckensteinB., LopezC., MinsonA.C., and StuddertM.J., Arch Virol123, 425–449, 1992.
JohnsonM.A., PrideauxC.T., KongsuwanK., SheppardM., and FaheyK.J., Arch Virol119, 181–198, 1991.
GuoP., ScholzE., MaloneyB., and WelniakE., Virology202, 771–781, 1994.
KongsuwanK., JohnsonM.A., PrideauxC.T., and SheppardM., Virus Genes7, 297–303, 1993.
KongsuwanK., JohnsonM.A., PrideauxC.T., and SheppardM., Virus Res29, 125–140, 1993.
WarkM.C., TannockG.A., and PyeD., J Biol Standard7, 73–80, 1979.
vanZijlM., QuintW., BriaireJ., deRoverT., GielkensA., and BernsA., J Virol62, 2191–2195, 1988.
AltschulS.F., GishW., MillerW., MyersE.W., and LipmanD.J., J Mol Biol215, 403–410, 1990.
GuyJ.S., BarnesH.J., MungerL.I., and RoseL. Avian Dis33, 316–323, 1989.
ReillyJ.D. and SilvaR.F., J Virol Methods41, 323–331, 1993.
Sheppard M.G., Prideaux C., Johnson M., Fahey K.J., York J.J., and Kongsuwan K., International Patent publication no. WO92/03554, 1992.
TelfordE.A.R., WatsonM.S., McBrideK., and DavisonA.J., Virology189, 304–316, 1992.
KozakM., Nucleic Acids Res15, 8125–8148, 1987.
ZelnikV., RossN.L.J., and PastorekJ., J Gen Virol75, 2747–2753, 1994.
PetrovskisE.A., TimminsJ.G., ArmentroutM.A., MarchioliC.C., YanceyR.J.Jr., and PostL., J Virol59, 216–223, 1986.
Velicer L.F., Brunovskis P., and Coussens P.M., International Patent publication no. WO92/03547, 1992.
SakaguchiM., UrakawaT., HirayamaY., MikiN., YamamotoM., and HiraiK., Virus Genes6, 365–378, 1992.
ZelnikV., DarteilR., AudonnetJ.D., SmithG.D., RiviereM., PastorekJ., and RossL.J.N., J Gen Virol74, 2151–2162, 1993.
ParcellsM.S., AndersonA.S., CantelloJ.L., and MorganR.W., J Virol68, 8239–8253, 1994.
YorkJ.J., SonzaS., BrandonM.R., and FaheyK.J., Arch Virol115, 147–162, 1990.
JohnsonM.A., TyackS.G., PrideauxC.T., KongsuwanK., and SheppardM., DNA Seq5, 191–194, 1995.
JohnsonM.A., TyackS.G., PrideauxC.T., KongsuwanK., and SheppardM., Virus Res35, 193–204, 1995.
BaumannR.P., YalamanchiliV.R.R., and O'CallaghanD.J., J Virol63, 1275–1283, 1989.
RixonF.J. and Ben-PoratT., Virology97, 151–163, 1979.
FukuchiK., TanakaA., SchiermanL.W., WitterR.L., and NonoyamaM., Proc Natl Acad Sci USA82, 751–754, 1985.
MaotaniK., KanamoriA., IkutaK., UedaS., KatoS., and HiraiS., J Virol58, 657–660, 1986.
DayhoffM.O., BarkerW.C., and HuntL.T., Methods Enzymol91, 524–545, 1983.
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The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence data-base and have been assigned the accession number U28832.
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Wild, M.A., Cook, S. & Cochran, M. A genomic map of infectious laryngotracheitis virus and the sequence and organization of genes present in the unique short and flanking regions. Virus Genes 12, 107–116 (1996). https://doi.org/10.1007/BF00572949
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DOI: https://doi.org/10.1007/BF00572949