Summary
The activity of acetylsalicylic acid-esterase (ASA-esterase) in blood and the degree of protein-salicylate binding were estimated in different age groups. The activity of ASA-esterase was assayed by the amount of salicylate released after incubation with ASA under standard conditions. Lower activity of the enzyme was found in neonates (48–49% of ASA hydrolysed after 60 min) than in older children (60–64% hydrolysed) and male adults (78–83% hydrolysed). The erythrocytes of adult females were less active than those from males. Protein-binding of salicylic acid (SA) was also lower in neonates than in adults (30.7% at SA 68.4 µg/ml, and 52% at 10.28 µg/ml SA, respectively). Even if the protein concentration in adult plasma were reduced to the level of the neonates by dilution, and if bilirubin were added to the mixture, the protein-binding was still greater in plasma from adults than neonates. These observations suggest that in the neonatal period the protein-affinity of certain substances may differ from that in adults.
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Breddin, K., Scharrer, I., Schepping, M.: Die Hemmung der Plättchenaggregation mit Acetylsalicylsäure. Münch. med. Wschr.113, 1284–1292 (1971)
Chignell, C.F., Vesell, E.S., Berlin, C.M.: The binding of sulphaphenazole to fetal, neonatal and adult plasma albumin. J. clin. Pharmacol. Therap.12, 897–901 (1971)
De Weck, A.L.: Acetylsalicylsäure, ein altes Arzneimittel in neuem Blickwinkel. Dtsch. med. Wschr.96, 1109–1115 (1971)
Ehrnebo, M., Agurell, S., Jalling, B., Boreus, L.O.: Age differences in drug binding by plasma proteins: studies on human fetuses, neonates and adults. Europ. J. clin. Pharmacol.3, 189–193 (1971)
Evans, G., Nishizawa, E.E., Packman, M.A.: The effect of acetylsalicylic acid (aspirin) on platelet function. Blood30, 550–556 (1967)
Ganshorn, A., Kurz, H.: Unterschiede zwischen der Proteinbindung Neugeborener und Erwachsener und ihre Bedeutung für die pharmakologische Wirkung. Naunyn-Schmiedeberg's Arch. Pharmak.260, 117–118 (1968)
Jacobsen, J., Thiessen, H., Brodersen, R.: Effect of fatty acids on the binding of bilirubin to albumin. Biochem. J.126, 7P (1972)
Jobin, F., Delange, J.M.: Aspirin and prednisone in microangiopathic haemolytic anaemia. Lancet1970 II, 208–210
Kucera, J.L., Bullock, F.J.: The binding of salicylates to plasma proteins from several animal species. J. Pharm. Pharmacol.21, 293–296 (1969)
Mac Millan, D.C.: Effect of salicylates on human platelets. Lancet1968 II, 1151–1152
Menguy, R., Desbaillets, L., Masters, Y.F., Okabe, S.: Evidence for sex-linked differences in aspirin metabolism. Nature239, 201–204 (1972)
Menon, J.S.: Aspirin and blood fibrinolysis. Lancet1970 II, 364
O'Brien, J.R.: Salicylates and platelet aggregation. Lancet1968 I, 779
Potter, G.D., Guy, J.L.: A micro method for analysis of plasma salicylates. Proc. Soc. exp. Biol. (N.Y.)116, 658–660 (1964)
Quick, A.J.: Bleeding time after aspirin ingestion. Lancet1968 I, 50
Sutor, A.H., Schindera, F., Jacobi, H., Künzer, W.: Haemolytic uremic syndrome: thrombocyturia after treatment with streptokinase and aspirin. Lancet1972 II, 762
Weiss, H.J., Aledort, L.M.: Impaired platelet connective-tissue reaction in man after aspirin ingestion. Lancet1967 I, 495–497
Windorfer, jun., A., Mihailova, K., Pringsheim, W.: Besteht erhöhte Gefahr einer Bilirubinencephalopathie im Neugeborenenalter durch eine medikamentöse Therapie? Dtsch. med. Wschr.98, 1260–1267 (1973)
Zucker, M.B., Peterson: Inhibition of adenosin-diphosphate induced secondary aggregation and other platelet functions by acetylsalicylic acid ingestion. Proc. Sec. exp. Biol. (N.Y.)127, 547–551 (1968)
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Windorfer, A., Kuenzer, W. & Urbanek, R. The influence of age on the activity of acetylsalicylic acid-esterase and protein-salicylate binding. Eur J Clin Pharmacol 7, 227–231 (1974). https://doi.org/10.1007/BF00560385
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DOI: https://doi.org/10.1007/BF00560385