Abstract
Starch gel electrophoresis of allozymes extracted from mycelium grown in submerged culture was used to identify and catalog lines ofLentinula edodes. Variability at 11 multiallelic loci was used to separate 91 lines collected from worldwide sources into 35 genotypic clases. Genotypic class 2 was the largest, with 18 lines; 18 other genotypic classes contained more than one line; and 16 genotypic classes contained only one line each. Three hybrids, with potential commercial value, were produced by crossing single-spore-derived monokaryons of different genotypes. Each of the three hybrids could be differentiated from the above 35 classes based on their multilocus allelic combinations. Multilocus enzyme analysis should aid the description, registration, and licensing of improved shiitake cultivars.
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References
Allendorf, F. W., Mitchell, N., Ryman, N., and Stahl, G. (1977). Isozyme loci in brown trout (Salmo trutta L.): Detection and interpretation from population data.Hereditas 86170.
Anonymous (1979). Implications of the plant patent act for the patentability of microorganisms.Md. Law Rev. 39(2):376.
Anonymous (1980a). Release: present situation of the edible mushroom in Japan, and the Plant Variety Protection System. Seeds and Seedlings Division, Agricultural Production Bureau, Ministry of Agriculture, Forestry and Fisheries, Tokyo.
Anonymous (1980b). Release: The standards of judgement of classification. Shiitake-mushroom,Lentinus edodes (Berk.) Sing. Seeds and Seedlings Division, Agricultural Production Bureau, Ministry of Agriculture, Forestry and Fisheries, Tokyo.
Bagwill, R. E. (1981). The legal aspects of plant tissue culture and patents.Environ. Expt. Bot. 21383.
Bels, P. J. (1962). An international centre for the investigation of mushroom strains.Mush. Sci. 5231.
Clayton, J. W., and Tretiak, D. N. (1972). Amine-citrate buffers for pH control in starch gel electrophoresis.J. Fish. Res. Bd. Can. 291169.
Cooper, I. P. (1982).Biotechnology and the Law Clark Boardman, New York.
Crespi, R. S. (1982).Patenting in the Biological Sciences Wiley, New York.
Darnall, D. W., and Klotz, I. M. (1972). Protein subunits: A table (revised edition).Arch. Biochem. Biophys. 1491.
Diehle, D. A., and Royse, D. J. (1986). Shiitake cultivation on sawdust: Evaluation of selected genotypes for biological efficiency and mushroom size.Mycologia 78929.
Eger, G. (1979). Mushroom strains can be protected.Mush. J. 7583.
Jodon, M. H., and Royse, D. J. (1979). Care and handling of cultures of the cultivated mushroom. Pa. Agr. Expt. Sta. Bull. 258.
Fritsche, G. (1983). BreedingAgaricus bisporus.Mush. J. 12249.
Lambert, L. F. (1932). Mushroom. U.S. plant patent 27 (Sept. 20).
Markert, C. L., and Faulhaber, I. (1965). Lactate dehydrogenase isozyme patterns of fish.J. Exp. Zool. 159319.
May, B., Roberts, D. W., and Soper, R. S. (1979a). Intraspecific genetic variability in laboratory strains ofEntomophthora as determined by enzyme electrophoresis.Exp. Mycol. 3289.
May, B., Wright, J. E., and Stoneking, M. (1979b). Joint segregation of biochemical loci in Salmonidae: Results from experiments withSalvelinus and review of the literature on other species.J. Fish. Res. Bd. Can. 361114.
Ridgway, G. J., Sherburne, S. U., and Lewis R. D. (1970). Polymorphism in the esterases of Atlantic herring.Trans. Am. Fish. Soc. 99147.
Robbins, W. A. (1961). Mushroom plant. U.S. patent 2,050 (Apr. 18).
Rogers, J. S. (1972). Measures of genetic similarity and genetic distance. In Wheeler, M. R. (ed.),Studies in Genetics, VII, Univ. Tex. Publ. 7213, pp. 145–153.
Royse, D. J. (1985). Effect of spawn run time and substrate nutrition on yield and size of the shiitake mushroom.Mycologia 75756.
Royse, D. J., and May, B. (1982a). Use of isozyme variation to identify genotypic classes ofAgaricus brunnescens.Mycologia 7493.
Royse, D. J., and May, B. (1982b). Genetic relatedness and its application in selective breeding ofAgaricus brunnescens.Mycologia 74569.
Royse, D. J., and May, B. P. (1986a). Mushroom plant. U.S. plant patent 5,772.
Royse, D. J., and May, B. P. (1986b). Mushroom plant. U.S. plant patent 5,773.
Royse, D. J., Spear, M. C., and May, B. (1983a). Cell line authentication and genetic relatedness of lines of the shiitake mushroom,Lentinus edodes.J. Gen. Appl. Microbiol. 29205.
Royse, D. J., Spear, M. C., and May, B. (1983b). Single and joint segregation of marker loci in the shiitake mushroom.Lentinus edodes.J. Gen. Appl. Microbiol. 29217.
Royse, D. J., Schisler, L. C., and Diehle, D. A. (1985). Shiitake mushrooms: Consumption, production and cultivation.Interdiscipl. Sci. Rev. 10329.
San Antonio, J. P. (1984). Origin and improvement of spawn of the cultivated mushroomAgaricus brunnescens Peck.Hort. Rev. 685.
Shattock, R. C., Tooley, P. W., and Fry, W. E. (1986). Genetics ofPhytophthora infestans: Characterization of single-oospore cultures from A1 isolates induced to self by intraspecific stimulation.Phytopathology 76407.
Shaw, C. R., and Prasad, R. (1970). Starch gel electrophoresis of isozymes—a compilation of recipes.Biochem. Genet. 4297.
Shechter, Y., Hall, R., Oelshlegel, F. J., Jr., Stahmann, M. A., Snider, R. D., Franke, R. G., Holton, R. W., Reddy, M. M., and Garber, E. D. (1973). Symposium on the use of electrophoresis in the taxonomy of algae and fungi.Bull. Torrey Bot. Club 100253.
Sneath, P. H. A., and Sokal, R. R. (1973).Numerical Taxonomy W. H. Freeman, New York.
Spieth, P. T. (1975). Population genetics of allozyme variation inNeurospora intermedia.Genetics 80785.
Tooley, P. W., Fry, W. E., and Villarreal Gonzalez, M. J. (1985). Isozyme characterization of sexual and asexualPhytophthora infestans population.J. Hered. 76431.
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Contribution No. 1602, Department of Plant Pathology, The Pennsylvania Agricultural Experiment Station. Authorized for publication October 1986 as Journal Series Paper No. 7524.
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Royse, D.J., May, B. Identification of shiitake genotypes by multilocus enzyme electrophoresis: Catalog of lines. Biochem Genet 25, 705–716 (1987). https://doi.org/10.1007/BF00556213
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DOI: https://doi.org/10.1007/BF00556213