Summary
Hood and Dare cultivars of soybean, Glycine max (L.) Merr., vary in their foliar response to ozone. The physiological basis of this variation was investigated as a function of leaf age through an analysis of ozone flux data, leaf developmental morphology, and analogue modelling techniques. At all concentrations (0.25–0.58 μl l-1) and exposure times (1–4 h), resistance to O3 flux in the gas phase of the diffusive pathway (i.e., boundary layer and stomate) did not account fully for variation in pollutant uptake rates into the leaf interior. Ozone molecules experienced a residual resistance to diffusion that is not shared by effluxing water vapor molecules. Residual resistance to O3 flux increased with pollutant concentration and exposure time and was associated with age-dependent differences in foliar O3 response. Leaf morphology data, including stomatal frequency and the ratio of internal to external surface area, did not help explain cultivar or age-dependent differences in O3 flux. The extent of foliar injury was not consistently related to the magnitude of O3 flux into the leaf interior. An analysis of the residual resistance to O3 flux suggests that the gas and liquid phase pathways for O3, water vapor, and carbon dioxide are not identical.
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References
Barton JR, McLaughlin SB, McConathy RK (1980) The effects of SO2 on components of leaf resistance to gas exchange. Environ Pollut Ser A 21:255–265
Bennett JH, Hill AC, Gates DM (1973) A model for gaseous pollutant sorption by leaves. J Air Pollut Control Assoc 23:957–962
Bierhuizen JF, Slayter RO (1964) An apparatus for the continuous and simultaneous measurement of photosynthesis and transpiration under controlled environmental conditions. SCIRO Div Land Res, Regional Surv Tech Paper 24:1–16
Black VJ, Unsworth MH (1980) Resistance analysis of sulphur dioxide fluxes to Vicia faba. Nature 282:68–69
Bobrov RA (1955) The leaf structure of Poa annua with observations on its smog sensitivity in Los Angeles County. Am J Bot 42:467–474
Butler LK, Tibbitts TW (1979) Stomatal mechanisms determining genetic resistance to ozone in Phaseolus vulgaris L. J Am Soc Hortic Sci 104:213–216
Cowan IR (1977) Stomatal behavior and Environment. In: RD Presten, HW Woolhouse (eds), Advances in Botanical Research. Academic Press, New York, p 117–228
Cowan IR, Troughton JH (1971) The relative role of stomata in transpiration and assimilation. Planta (Berl) 97:325–336
Craker LE, Starbuck JS (1973) Leaf age and air pollutant susceptibility: uptake of ozone and sulfur dioxide. Environ Res 6:91–94
Downs RJ, Hellmers H (1975) Enviroment and the Experimental Control of Plant Growth. Academic Press, New York
Engle RL, Gabelman WH (1966) Inheritance and mechanism for resistance to ozone damage in onion Allium cepa L. Proc Am Soc Hortic Sci 89:423–430
Evans LS, Ting IP (1974) Ozone sensitivity of leaves: relationship to leaf water content, gas transfer resistance, and anatomical characteristics. Am J Bot 61:592–597
Feder N, O'Brien TP (1968) Plant microtechnique: some principles and new methods. Am J Bot 55:123–142
Fisher D (1967) An unusual layer of cells in the mesophyll of the soybean leaf. Bot Gaz 128:215–218
Gaastra P (1959) Photosynthesis of crop plants as influenced by light, carbon dioxide, temperature, and stomatal diffusion resistance. Meded Landbowhoogesch Wageningen 13:1–68
Glater RB (1956) Smog damage to ferns in the Los Angeles Area. Phytopathology 46:696–698
Heath RL (1975) Ozone. In: JB Mudd, TT Kozlowski (eds) Responses of Plants to Air Pollution. Academic Press, New York, p 23–41
Heath RL (1980) Initial events in injury to plants by air pollutants. Annu Rev Plant Physiol 31:395–431
Hill AC (1971) Vegetation: a sink for atmospheric pollutants. J Air Pollut Control Assoc 21:341–346
Lamoreau RJ, Chaney WR, Brown KM (1978) The plastochron index: a review after two decades of use. Am J Bot 65:586–593
Leuning R, Neumann HH, Thurtell GW (1979) Ozone uptake by corn (Zea mays L.): A general approach. Agric Meterol 20:115–135
Levitt J (1980) Responses of Plants to Environmental Stresses. I. Chilling, Freezing, and High Temperature Stresses. Second ed, Academic Press, New York
Mansfield T (1973) The role of stomata in determining the responses of plants to air pollutants. In: H Smith (ed) Current Advances in Poant Science. Pergammon Press, New York, pp 11–20
McLaughlin SB, Taylor GE (1981) Relative humidity important modifier of pollutant uptake by plants. Science 211:167–169
Nobel PS (1974) Introduction to Biophysical Plant Physiology. WH Freeman and Co. San Francisco
Nobel PS, Zaragoza LJ, Smith WK (1975) Relation between mesophyll surface area, photosynthetic rate, and illumination level during development for leaves of Plectranthus parviflorus Henckel. Plant Physiol 55:1067–1070
O'Dell RA, Taheri M, Kabel RL (1977) A model for uptake of pollutants by vegetation. J Air Pollut Control Assoc 27:1104–1109
Rand RH (1977) Gaseous diffusion in the leaf interior. Trans ASAE 20:701–704
Rand RH (1978) A theoretical analysis of CO2 absorption in sun versus shade leaves. J Biomech Engin 100:20–24
Runeckles VC (1974) Dosage of air pollutants and damage to vegetation. Environ Conserv 4:305–308
Sestak Z, Catsky J, Jarvis PG (1971) Plant Photosynthetic Production. Manual of Methods. Dr/W Junk. NU Publ, The Hague
Snedecor GW, Cochran WG (1967) Statistical Methods. Sexth ed The Iowa University Press, Ames Iowa
Tanton TW, Crowdy SH (1972) Water pathways in higher plants. J Exp Bot 27:619–625
Taylor GE Jr (1978) Plant and leaf resistance to gaseous air pollution stress. New Phytol 80:523–534
Tingey DT, Fites RC, Wickliff C (1973) Foliar sensitivity of soybeans to ozone as related to several leaf parameters. Environ Pollut 4:183–192
Tingey DT, Fites RC, Wickliff C (1976) Differential foliar sensitivity of soybean cultivars to ozone associated with differential enzyme activities. Physiol Plant 37:69–72
Tingey DT, Reinert RA, Carter HB (1972) Soybean cultivars: acute foliar response to ozone. Crop Sci 12:268–270
Tingey DT, Taylor GE Jr (1981) Variation in plant response to ozone: a conceptual model of physiological events. In: MH Unsworth, DP Ormrod (eds), Effects of Gaseous Air Pollution in Agriculture and Horticulture, Butterworth Press, New York (in press)
Townsend AM (1974) Sorption of ozone by nine shade tree species. J Am Soc Hortic Sci 99:206–208
Turrell FM (1936) The area of the internal exposed surface of dicotyledon leaves. Am J Bot 23:255–264
Turrell FM (1942) A quantitative morphological analysis of large and small leaves of alfalfa, with special reference to internal surface. Am J Bot 22:400–415
Tyree MT, Yianoulis P (1980) The site of water evaporation from sub-stomatal cavities, liquid path resistances and hydroactive stomatal closure. Ann Bot 46:175–193
Unsworth MH, Biscoe PV, Black V (1976) Analysis of gas exchange between plants and polluted atmospheres. In: TA Mansfield (ed), Effects of Air Pollutants on Plants, Cambridge University Press, New York, p 5–16
Wesely ML, Eastman JA, Cook DR, Hicks BB (1978) Daytime variations of ozone eddy fluxes to maize. Boundary-Layer Meteorol 15:361–373
Weston GD, Cass DD (1973) Observations on the development of the paraveinal mesophyll of soybean leaves. Bot Gaz 134:232–235
Williams JA (1973) A considerably improved method for preparing plastic epidermal imprints. Bot Gaz 134:87–91
Yingjajaval S (1976) Ozone inhibition of transpiration and photosynthesis of two soybean (Glycine max (L.) Merr.) cultivars. Masters Thesis, Oregon State University, Corvallis Oregon
Yocum CS, Lommen PW (1975) Mesophyll resistances. In: DM Gates, RB Schmerl (eds), Perspectives of Biophysical Ecology Springer, Berlin Heidelberg New York, p 45–54
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Taylor, G.E., Tingey, D.T. & Ratsch, H.C. Ozone flux in Glycine max (L.) Merr.: Sites of regulation and relationship to leaf injury. Oecologia 53, 179–186 (1982). https://doi.org/10.1007/BF00545661
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DOI: https://doi.org/10.1007/BF00545661