Abstract
Glycerol-extracted insect fibrillar muscle fibres in rigor exhibited both an elastic and a plastic phase in the length-tension diagram. The transition between these phases took place at a critical tension, the “yield point” or elastic limit. In the plastic phase the apparent static elastic modulus became zero, whereas the immediate elastic modulus (measured by rapid length changes completed within 4 ms) exhibited no abrupt change at the yield point. The tension value of the yield point (but not immediate stiffness) was lowered by addition of AMP-PNP and was partially restored by washing out AMP-PNP. The dependence of the critical tension at which plastic flow begins on cooperative cross bridge behaviour is discussed in terms of breaking and reforming acto-myosin linkages. Evidence is presented that addition of AMP-PNP induces slippage of cross bridges on the actin filament by affecting the interaction between myosin and actin.
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Abbott, R. H., Leech, A. R.: Persistance of adenylate kinase and other enzymes in glycerol extracted muscle. Pflügers Arch. 344, 233–243 (1973)
Abbott, R. H., Steiger, G. J.: Temperature and amplitude dependence of tension transients in glycerinated skeletal and insect fibrillar muscle. J. Physiol. (Lond.) 266, 13–42 (1977)
Barrington Leigh, J., Holmes, K. C., Mannherz, H. G., Rosenbaum, G., Eckstein, F., Goody, R.: Effects of ATP analogs on the low-angle X-ray diffraction pattern of insect flight muscle. Cold Spr. Harb. Symp. quant. Biol. 37, 443–447 (1973)
Beinbrech, G., Kuhn, H. J., Herzig, J. W., Rüegg, J. C.: Evidence for two attached myosin cross bridges states of different potential energy. Cytobiology 12, 385–396 (1976)
Birshtein, T. M., Ptitsyn, O. B.: Conformations of macromolecules. New York: Interscience 1966
Feldhaus, P., Fröhlich, T., Goody, R. S., Isakov, M., Schirmer, R. H.: Synthetic inhibitors of adenylate kinases in the assays for ATPases and phosphokinases. Europ. J. Biochem. 57, 197–204 (1975)
Goody, R. S., Holmes, K. C., Mannherz, H. G., Barrington Leigh, J., Rosenbaum, G.: X-ray diffraction studies of insect flight muscle with ATP analogues. Biophys. J. 15, 687–704 (1975)
Goody, R. S., Barrington Leigh, J., Mannherz, H. G., Tregear, R. T., Rosenbaum, G.: X-ray titration of binding Β, γ-imido-ATP to myosin in insect flight muscle. Nature (Lond.) 262, 613–614 (1976)
Heinl, P., Kuhn, H. J., Rüegg, J. C.: Tension responses to quick length changes of glycerinated skeletal muscle fibres from the frog and tortoise. J. Physiol. (Lond.) 237, 243–258 (1974)
Huxley, H. E.: The mechanism of muscular contraction. Science 164, 1356–1366 (1969)
Huxley, A. F., Simmons, R. M.: Proposed mechanism of force generation in striated muscle. Nature (Lond.) 233, 535 (1971)
Jewell, B. R., Rüegg, J. C.: Oscillatory contraction of insect fibrillar muscle after glycerol extraction. Proc. roy. Soc. B 164, 428 (1966)
Kawai, M., Brandt, P. W.: Two rigor states in skinned crayfish single muscle fibers. J. gen. Physiol. 68, 267–280 (1976)
Kuhn, H. J.: Transformation of chemical energy into mechanical energy by glycerol-extracted fibres of insect flight muscle in the absence of nucleosidetriphosphate-hydrolysis. Experentia (Basel) 29, 1086–1088 (1973)
Kuhn, H. J.: Reversible transformation of mechanical work into chemical free energy by stretch dependent binding of AMP-PNP in glycerinated fibrillar muscle fibres. Symposium on insect flight muscle, Oxford 1977, p. 307. Amsterdam: Elsevier/North Holland 1977
Lowy, J., Mulvany, M. J.: Effect of calcium on mechanical properties of rigor frog sartorius muscles. J. Physiol. (Lond.) 233, 41–43 (1973)
Lymn, R. W., Huxley, H. E.: X-ray diagrams from skeletal muscle in the presence of ATP analogs. Cold Spr. Harb. Symp. quant. Biol. 37, 449–453 (1973)
Maréchal, G.: Rigidité et plasticité du muscle strié en contracture iodoacétique. Arch. int. Physiol. Biochim. 68, 740–760 (1960)
Marston, S. B., Rodger, C. D., Tregear, R. T.: Changes in muscle cross bridges when Β, γ-imido-ATP binds to myosin. J. molec. Biol. 104, 263–276 (1976)
Mulvany, M. J.: Mechanical properties of frog skeletal muscles in iodoacetic acid rigor. J. Physiol. (Lond.) 252, 319–334 (1975)
Reedy, M. K., Holmes, K. C., Tregear, R. T.: Induced changes in orientation of the cross bridges of glycerinated insect flight muscle. Nature (Lond.) 207, 1279 (1965)
Rome, E.: Relaxation of glycerinated muscle: low-angle X-ray diffraction studies. J. molec. Biol. 65, 331–345 (1972)
White, D. C. S.: Rigor contraction in glycerinated insect flight and vertebrate muscle. J. Physiol. (Lond.) 208, 583 (1970)
Yount, R. G., Babock, D., Ballantyne, W., Ojala, D.: Adenyl imidodiphosphate, an adenosine triphosphate analog containing a P-N-P linkage. Biochemistry 10, 2484–2489 (1971)
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Kuhn, H.J. Cross bridge slippage induced by the ATP analogue AMP-PNP and stretch in glycerol-extracted fibrillar muscle fibres. Biophys. Struct. Mechanism 4, 159–168 (1978). https://doi.org/10.1007/BF00539229
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DOI: https://doi.org/10.1007/BF00539229