Abstract
We have studied the influence of a temperature-sensitive cdc2-1 mutation in DNA polymerase δ on the selection-induced mutation occurring at the LYS-2 locus in the yeast Saccharomyces cerevisiae. It was found that in cells plated on synthetic complete medium lacking only lysine, the numbers of Lys+ revertant colonies accumulated in a time-dependent manner in the absence of any detectable increase in cell number. When cdc2-1 mutant cells, after selective plating, were incubated at the restrictive temperature of 37°C for 5 h daily for 7 days, the frequency of an adaptive reversion of lys - →Lys+ was significantly higher than the frequency in cells incubated only at the permissive temperature, or in wild-type cells incubated either at 23°C or 37°C. Therefore, when the proof-reading activity of DNA polymerase δ is impaired under restrictive conditions, the frequency of adaptive mutations is markedly enhanced.
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Communicated by F.K. Zimmermann
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Baranowska, H., Policińska, Z. & Jachymczyk, W.J. Effects of the CDC2 gene on adaptive mutation in the yeast Saccharomyces cerevisiae . Curr Genet 28, 521–525 (1995). https://doi.org/10.1007/BF00518164
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DOI: https://doi.org/10.1007/BF00518164