Summary
The localization of immunoreactive angiotensin I-converting enzyme (ACE) has been investigated at the optical and ultrastructural level with anti-human ACE antibodies in the human kidney and small intestine. In both tissues ACE was found in blood vessels and in extravascular situation in the absorptive epithelial cells of intestinal mucosa and renal proximal tubules. Ultrastructural immunohistochemistry showed that in intestinal and renal proximal tubular cells ACE was prominent in microvilli and brush borders. In the kidney ACE was also present on the basolateral part of the plasmalemmal membrane, where it may contribute to the regulation of angiotensin II-dependant absorption processes. Intracellular positivities were also observed inside the renal vascular endothelial and proximal tubular cell in endoplasmic reticulum and nuclear envelope reflecting the synthesis and the cellular processing of ACE. The intestinal microvascular endothelium was strongly labeled suggesting that the mesenteric circulation is an important site for the production of angiotensin II. Vascular endothelial ACE was also detected in the peritubular but not glomerular capillaries of the kidney.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Alhenc-Gelas F, Weare JA, Johnson RL Jr., Erdös EG (1983) Measurement of human converting enzyme by direct radioimmunoassay. J Lab Clin Med 101:83–96
Bariety J, Druet P, Laliberté F, Sapin C, Belair MF, Paing M (1978) Ultrastructural reabsorption of endogenous albumin in normal rat. Lab Invest 38:175–180
Bourel D, Beaumont C, Rissel M, Guguen-Guillouzo C, Guillouzo A (1981) Ultrastructural localization of alpha-fetoprotein in isolated and cultured rat hepatocytes. J Ultrastruct Res 74:217–222
Brown GP, Gouglas JG (1982) Angiotensin II-binding sites on isolated rat renal brush border membranes. Endocrinology 111:1830–1836
Brown GP, Douglas JG (1983) Angiotensin II-binding sites in rat and primate isolated renal tubular basolateral membranes. Endocrinology 112:2007–2014
Caldwell PRB, Seegal BC, Hsu KC (1976) Angiotensin-converting enzyme: vascular endothelial localization. Science 191:1050–1051
Camilleri JP, Hinglais N, Bruneval P, Bariety J Tricottet V, Rouchon M, Mancilla-Jimenez R, Corvol P, Menard J (1984) Renin storage and cell differentiation in juxtaglomerular cell tumors: an immunohistochemical and ultrastructural study of three cases. Hum Pathol 15:1069–1079
Carone FA, Peterson DR, Flouret G (1982) Renal tubular processing of small peptid hormones. J Lab Clin Med 100:1–14
Defendini R, Zimmerman EA, Weare JA, Alhene-Gelas F, Erdös EG (1983) Angiotensin-converting enzyme in epithelial and neuroepithelial cells. Neuroendocrinology 37:32–40
Di Salvo J, Montefusco CB (1971) Conversion of angiotensin I to angiotensin II in the canine mesenteric circulation. Am J Physiol 221:1576–1579
Erdös EG (1975) Angiotensin I-converting enzyme. Circ Res 36:247–255
Erdös EG (1979) Kininases. In Bradykinin, kallidin, and kallikrein. In: Erdös EG (ed) Handbook of experimental pharmacology, suppl to vol XXV. Springer, Berlin Heidelberg New York, pp 427–487
Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291
Guder WG, Ross BD (1984) Enzyme distribution along the nephron. Kidney Int 26:101–111
Guesdon JL, Ternynck T, Avrameas S (1979) The use of avidinbiotin interaction in immunoenzymatic techniques. Histochem Cytochem 27:1131–1139
Hall ER, Kato J, Erdös EG, Robinson CJG, Oshima G (1976) Angiotensin I converting enzyme in the nephron. Life Sci 18:1299–1303
Jensen PK, Steven K (1977) Angiotensin II induced reduction of peritubular capillary diameter in the rat kidney. Pflügers Arch 371:245–250
Levens NR, Peach MJ, Carey RM, Poat JA, Munday KA (1980) Stimulation of intestinal sodium and water transport in vivo by angiotensin II and analogs. Endocrinology 107:1946–1953
Ng KKF, Vane JR (1967) Conversion of angiotensin I to angiotensin II, Nature 216:762–766
Ryan US, Ryan JW, Whitaker C, Chiu A (1976) Localization of angiotensin-converting enzyme (Kininase II). II. Immunocytochemistry and immunofluorescence. Tissue Cell 8:125–145
Schuster VL, Kokko JP, Jacobson HR (1984) Angiotensin II directly stimulates sodium transport in rabbit proximal convoluted tubules. J Clin Invest 73:507–515
Skeggs LT, Kahn JR, Shumway NP (1956) Preparation and function of the hypertensin-converting enzyme. J Exp Med 103:295–299
Sternberger LA (1979) Immunocytochemistry, vol 1, 2nd edn. John Wiley and Sons, New York
Steven K, Thorpe DH (1977) Angiotensin II: intratubular and renal capillary pressure regulation in rat kidney. Acta Physiol Scand 101:394–403
Takada Y, Unno M, Hiwada K, Kokubu T (1981) Immunological and immunofluorescent studies of human angiotensin-converting enzyme. Clin Sci 61:2535–2565
Takada Y, Hiwada K, Unno M, Kokubu T (1982) Immunocytochemical localization of angiotensin converting enzyme at the ultrastructural level in the human lung and kidney. Biomed Res 3:169–174
Taugner R, Ganten D (1982) The localization of converting enzyme in kidney vessels of the rat. Histochemistry 75:191–201
Ward PE, Sheridan MA (1982) Angiotensin I converting enzyme of rat intestinal and vascular surface membrane. Biochim Biophys Acta 716:208–216
Ward PE, Sheridan MA (1983) Immunoelectrophoretic analysis of renal and intestinal brush border converting enzyme. Biochem Pharmacol 32:265–274
Ward PE, Sheridan MA, Hammon KJ, Erdös EG, (1980) Angiotensin I converting enzyme (Kininase II) of the brush border of human and swine intestine. Biochem Pharmacol 29:1525–1529
Weare JA, Gafford JT, Lu HS, Erdös EG (1982) Purification of human kidney angiotensin I converting enzyme using reverse immunoadsorption chromatography. Anal Biochem 123:310
Yasui T, Alhenc-Gelas F, Corvol P, Menard J (1984) Angiotensin I converting enzyme in amniotic fluid. J Lab Clin Med 104:741–751
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bruneval, P., Hinglais, N., Alhenc-Gelas, F. et al. Angiotensin I converting enzyme in human intestine and kidney. Histochemistry 85, 73–80 (1986). https://doi.org/10.1007/BF00508656
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00508656