Summary
Attempts were made to entrap enzymes or microbial cells with water-insoluble photo-crosslinkable resin prepolymers of different types in organic solvent systems in the presence or absence of water. Acetone-dried cells of Artbrobacter simplex immobilized in a maleic polybutadiene gel (PBM-2000) converted hydrocortisone to prednisolone in a phosphate buffer. 4-Androstene-3,17-dione was converted to androst-1,4-diene-3,17-dione in benzene-n-heptane solution by Nocardia rhodocrous which was immobilized by a hydrophobic prepolymer, ENTP-2000. The ENTP-2000 had been synthesized from poly(propylene glycol)-2000, hydroxyethylacrylate and isophorone diisocyanate. Even enzymes catalyzing aqueous phase reactions, such as catalase and invertase, were immobilized in a polybutadiene resin (PB-200k) to give active gel-entrapped preparations. The cells and enzymes immobilized in these hydrophobic resins exhibited moderate activities compared with those of the free cells and enzymes.
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Abbreviations
- 4-AD:
-
4-androstene-3,17-dione
- ADD:
-
androst-1,4-diene-3,17-dione
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Omata, T., Tanaka, A., Yamane, T. et al. Immobilization of microbial cells and enzymes with hydrophobic photo-crosslinkable resin prepolymers. European J. Appl. Microbiol. Biotechnol. 6, 207–215 (1979). https://doi.org/10.1007/BF00508092
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DOI: https://doi.org/10.1007/BF00508092