Summary
Plant cells, entrapped in agarose or alginate, were permeabilized in a new procedure that retain cell viability, permitting the possibility of reusing biomass after release of intracellular products. Dimethylsulfoxide (DMSO) was used to make the cells permeable. The activity of isocitrate dehydrogenase, an intracellular enzyme, was used as an indicator of plasma membrane permeability. Cells from three plant species require different concentrations of DMSO for complete permeabilization (i.e., for maximal isocitrate dehydrogenase activity). Cells of Catharanthus roseus permeabilized with up to 5% DMSO remained viable, and released 85–90% of the intracellularly stored products (ajmalicine isomers). In model production systems, C. roseus cells entrapped in agarose or alginate beads were intermittently permeabilized for release of products in a cyclic process. An increase in product yield was observed for each cycle because of increase in biomass (cell growth) within the beads.
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Brodelius, P., Nilsson, K. Permeabilization of immobilized plant cells, resulting in release of intracellularly stored products with preserved cell viability. European J. Appl. Microbiol. Biotechnol. 17, 275–280 (1983). https://doi.org/10.1007/BF00508020
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DOI: https://doi.org/10.1007/BF00508020