Summary
Phanerochaete chrysosporium was not able to degrade purified Kraft lignin under nitrogen limitation when the secondary growth phase was limited by slow feeding of glucose. The formation of new mycelia was found to be the primary phenomenon during the secondary phase. Lignin was degraded only when excess energy was available. Using a homogeneous, thin layer of cells, the physical contact between the new cells and lignin was improved and the local oxygen-limited conditions were avoided, resulting in a rapid and total bioalteration of lignin. Completely water- and acid-soluble products, which comprised about 30–35% of the original lignin absorbance, were formed.
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Leisola, M., Ulmer, D., Haltmeier, T. et al. Rapid solubilization and depolymerization of purified Kraft lignin by thin layers of Phanerochaete chrysosporium . European J. Appl. Microbiol. Biotechnol. 17, 117–120 (1983). https://doi.org/10.1007/BF00499862
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DOI: https://doi.org/10.1007/BF00499862