Summary
CO2 gas, evolved during alcohol fermentation using immobilized yeast, causes several undesirable problems in a packed-bed bioreactor installed vertically as it increases the dead space and causes hydrostatic pressure. In order to reduce this “CO2 gas phase effect” which lowers the efficiency of ethanol production, a shallow, horizontal packed-bed bioreactor has been developed with a free space above the gel bed. The horizontal packed-bed bioreactor was 1.5 times more productive than the vertical packed-bed bioreactor when operated continuously. Yeast cells immobilized in calcium alginate gel reached a steady state much quicker than those immobilized in polyacrylamide gels. In the horizontal packed-bed bioreactors, calcium alginate gel was also superior to polyacrylamide gel with respect to ethanol productivity. The profiles of both glucose and ethanol concentrations against axial sampling sites suggested that the horizontal packed-bed bioreactor was similar to a plug flow reactor. The mean gel size gradually increased upstream (1.9 mm to 3.3 mm). With the economic production of ethanol in view, the published data on different continuous alcohol production processes have been compared by plotting their productivities (y-coordinate) against the ethanol concentrations in the effluents (x-coordinate) for the dilution rate or space velocity at which the yield of ethanol from glucose was 95%. The horizontal packed-bed bioreactor has a very high performance which makes this bioreactor promising for the economic production of ethanol.
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References
Arcuri JE, Worden RM, Schumate II SE (1980) Ethanol production by immobilized cells of Zymomonas mobilis. Biotechnol Lett 2:499–504
Chibata I, Tosa T, Sato T (1974) Immobilized aspartase-containing microbial cells: preparation and enzymic properties. Appl Microbiol 27:878–885
Ghose TK, Tyagi RD (1979) Rapid ethanol fermentation of cellulose hydrolysate I. Batch versus continuous systems. Biotechnol Bioeng 21:1387–1400
Ghose TK, Bandyopadhyay KK (1980) Rapid ethanol fermentation in immobilized yeast cell reactor. Biotechnol Bioeng 22: 1489–1496
Greenshields RN, Smith EL (1971) Tower-fermentation systems and their applications. The Chem Engr May 1971:182–190
Grote W, Lee KJ, Rogers PL (1980) Continuous ethanol production by immobilized cells of Zymomonas mobilis. Biotechnol Lett 2:481–486
Hartline FF (1979) Lowering the cost of alcohol. Science 206, October:41–42
Kierstan M, Bucke C (1977) The immobilization of microbial cells, subcellar organelles and enzymes in calcium alginate gels. Biotechnol Bioeng. 19:487–497
Lee KJ, Tribe DE, Rogers, PL (1979) Ethanol production by Zymomonas mobilis in continuous culture at high glucose con-contrations. Biotechnol Lett 1:421–426
Moo-Young M, Lamptey J, Robinson CW (1980) Immobilization of yeast cells on various supports for ethanol production. Biotechnol Lett 2:541–548
Navarro JM, Durand G (1977) Modification of yeast metabolism by immobilization onto porous glass. Eur J Appl Microbiol 4:243–254
Siess MH, Divies C (1981) Behaviour of Saccharomyces cerevisiae cells entrapped in a polyacrylamide gel and performing alcoholic fermentation. Eur J Appl Microbiol Biotechnol 12:10–15
Sitton OC, Gaddy JL (1980) Ethanol production in an immobilized cell reactor. Biotechnol Bioeng 22:1735–1748
Wada M, Kato J, Chibata I (1980) Continuous production of ethanol using immobilized growing yeast cells. Eur J Appl Microbiol Biotechnol 10:275–287
Wada M, Kato J, Chibata I (1981) Continuous production of ethanol in high concentration using immobilized growing yeast cells. Eur J Appl Microbiol Biotechnol 11:67–71
Yamané T, Nakatani H, Sada E, Omata T, Tanaka A, Fukui S (1979) Steroid bioconversion in water-insoluble organic solvents: 101-1 by free microbial cells and by cells entrapped in hydrophilic or lipophilic gels. Biotechnol Bioeng 21:2133–2145
Yamané T, Shiotani T (1981) Interrelationship among specific rates of cell growth, substrate consumption and metabolite formation ins ome simple microbial reactions producing primary metabolites. Biotechnol Bioeng 23:1373–1387
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Shiotani, T., Yamané, T. A horizontal packed-bed bioreactor to reduce CO2 gas holdup in the continuous production of ethanol by immobilized yeast cells. European J. Appl. Microbiol. Biotechnol. 13, 96–101 (1981). https://doi.org/10.1007/BF00499695
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DOI: https://doi.org/10.1007/BF00499695